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Fert2 (BC058100) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210538
Fert2 (Myc-DDK-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $700.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fert2
Synonyms AV082135; C330004K01Rik; Fert; Fert2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210538 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGATTTGGGAGTGACCTGAAGAACTCACAGGAAGCTGTGTTAAAGTTGCAAGACTGGGAACTACGGT
TGCTGGAGACAGTGAAGAAATTTATGGCTCTGAGAATAAAGAGTGATAAAGAATATGCATATACTCTACA
GAATCTTTGTAATCAAGTTGATAAAGAAAGCACTGTGCAAGTGAATTATGTCAGCAACGTGTCCAAGGTC
ACAAAGACAGAATTGGAGAAATTAAAATCCAGCTATCGACAATTAATAAAAGAAATGAATTCTGCCAAAG
AGAAATATAAAGAAGCCTTAGCGAAAGGGAAGGAAACAGAAAAGGCCAAGGAGCGCTATGACAAAGCTAC
AATGAAGCTTCATATGCTGCATAATCAGTATGTGTTGGCACTGAAGGGGGCACAGCTCCATCAGAGCCAG
TACTATGATACCACACTTCCTCTGCTGCTGGACTCCGTGCAAAAGATGCAAGAAGAAATGATAAAAGCAC
TAAAAGGTATATTTGATGACTATAGCCAGATAACCAGCCTTGTTACAGAGGAAATAGTGAATGTCCATAA
AGAGATTCAAATGTCTGTTGAACAGATAGACCCTAGCACCGAATATAATAATTTCATAGATGTTCACAGA
ACAACAGCTGCTAAAGAACAAGAAATCGAATTTGATACTTCCCTATTAGAAGAAAATGAAAATCTTCAGG
CAAATGAGATCATGTGGAATAATTTAACAGCAGACAGTTTGCAAGTCATGTTGAAGACTTTAGCGGAAGA
GCTCACACAGACCCAGCAGATGCTTTTACACAAGGAGGCGGCCGTCCTGGAGCTAGAGAAGAGGATTGAA
GAATCTTTTGAGACCTGTGAAAAGAAGTCTGACATTGTGCTTCTTCTGGGCCAAAAACAGGCACTTGAAG
AGTTGAAACAGTCAGTCCAGCAGTTGCGATGCACTGAGGCAAAGTGTGCAGCACAGAAAGCTTTGCTGGA
GCAAAAAGTACAGGAAAATGATGGGAAAGAACCACCTCCTGTGGTGAATTACGAAGAAGACGCGCGGTCA
GTCACATCCATGGAAAGAAAGGAGAGGCTATCCAAATTTGAGTCTATTCGTCATTCAATTGCTGGGATAA
TTAAGTCTCCAAAGTCGGTACTCGGATCTTCAACACAGGTGTGTGATGTGATCTCTGTGGGTGAGAGGCC
CCTGGCGGAGCATGACTGGTACCATGGTGCCATCCCCAGGATAGAGGCACAGGAACTGCTGAAGCAGCAA
GGAGACTTCCTGGTGCGGGAGAGCCATGGGAAACCTGGTGAATATGTCCTTTCTGTATATTCTGACGGAC
AAAGGAGGCACTTTATCATACAATTTGTCGATAATCTGTATCGATTCGAGGGCACCGGGTTTTCAAACAT
TCCCCAGCTTATAGATCACCACTTCAATACAAAGCAAGTCATCACCAAGAAGTCTGGGGTGGTTCTGCTC
AACCCCATCCCAAAGGATAAGAAATGGGTTCTCAATCATGAAGATGTTTCATTGGGAGAATTACTGGGCA
AGGGGAATTTTGGTGAAGTGTATAAGGGCACACTAAAGGATAAAACTCCTGTTGCCATTAAAACGTGCAA
GGAAGACCTTCCTCAGGAATTAAAAATAAAGTTTCTACAGGAAGCCAAAATTCTGAAGCAATATGATCAT
CCCAATATTGTCAAACTGATAGGCGTGTGCACACAAAGACAGCCTGTCTACATCATTATGGAACTGGTCC
CAGGGGGTGATTTTCTGACCTTCCTGAGGAAGAGGAAGGACGAGCTGAAGCTGAAGCAGTTGGTGAGATT
TTCCTTGGACGTTGCTGCTGGCATGTTGTATCTCGAGAGCAAGAACTGCATTCACAGGGACCTGGCTGCA
CGGAACTGCCTGGTGGGTGAAAATAATACTCTGAAAATCAGTGACTTTGGAATGTCTCGGCAAGAAGACG
GTGGAGTGTATTCATCTTCTGGCTTAAAGCAGATTCCTATTAAATGGACAGCACCGGAAGCTCTTAATTA
TGGGAGATACAGTTCTGAAAGTGACGTGTGGAGCTTCGGCATCCTCCTCTGGGAGACCTTCAGCCTAGGA
GTCTGCCCGTACCCTGGAATGACAAACCAGCAAGCGCGAGAGCAAGTGGAGAGAGGATACCGGATGTCAG
CCCCACAGAACTGTCCAGAGGAGGTTTTTACAATCATGATGAAGTGTTGGGATTACAAGCCTGAAAACCG
CCCTAAGTTCAACGACCTTCACAAAGAGCTCACTGTCATCAAGAAGATGATCACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210538 protein sequence
Red=Cloning site Green=Tags(s)

MGFGSDLKNSQEAVLKLQDWELRLLETVKKFMALRIKSDKEYAYTLQNLCNQVDKESTVQVNYVSNVSKV
TKTELEKLKSSYRQLIKEMNSAKEKYKEALAKGKETEKAKERYDKATMKLHMLHNQYVLALKGAQLHQSQ
YYDTTLPLLLDSVQKMQEEMIKALKGIFDDYSQITSLVTEEIVNVHKEIQMSVEQIDPSTEYNNFIDVHR
TTAAKEQEIEFDTSLLEENENLQANEIMWNNLTADSLQVMLKTLAEELTQTQQMLLHKEAAVLELEKRIE
ESFETCEKKSDIVLLLGQKQALEELKQSVQQLRCTEAKCAAQKALLEQKVQENDGKEPPPVVNYEEDARS
VTSMERKERLSKFESIRHSIAGIIKSPKSVLGSSTQVCDVISVGERPLAEHDWYHGAIPRIEAQELLKQQ
GDFLVRESHGKPGEYVLSVYSDGQRRHFIIQFVDNLYRFEGTGFSNIPQLIDHHFNTKQVITKKSGVVLL
NPIPKDKKWVLNHEDVSLGELLGKGNFGEVYKGTLKDKTPVAIKTCKEDLPQELKIKFLQEAKILKQYDH
PNIVKLIGVCTQRQPVYIIMELVPGGDFLTFLRKRKDELKLKQLVRFSLDVAAGMLYLESKNCIHRDLAA
RNCLVGENNTLKISDFGMSRQEDGGVYSSSGLKQIPIKWTAPEALNYGRYSSESDVWSFGILLWETFSLG
VCPYPGMTNQQAREQVERGYRMSAPQNCPEEVFTIMMKCWDYKPENRPKFNDLHKELTVIKKMIT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC058100
ORF Size 2295 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC058100, AAH58100
RefSeq Size 2948 bp
RefSeq ORF 2297 bp
Locus ID 14158
Cytogenetics 17 E1.1
MW 87.8 kDa
Summary Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3 according to PubMed:10878010 and PubMed:19159681, but clearly plays a redundant role in STAT3 phosphorylation. According to PubMed:11134346, cells where wild type FER has been replaced by a kinase-dead mutant show no reduction in STAT3 phosphorylation. Phosphorylates TMF1. Isoform 3 lacks kinase activity.UniProtKB/Swiss-Prot Function
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"BC058100" in other vectors (4)
SKU Description Size Price
MC220721 Fert2 (untagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507), (10ug) 10 ug
$771.00
MG210538 Fert2 (tGFP-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507) 10 ug
$900.00
MR210538L3 Lenti ORF clone of Fert2 (Myc-DDK-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507) 10 ug
$1,000.00
MR210538L4 Lenti ORF clone of Fert2 (mGFP-tagged) - Mouse fer (fms/fps related) protein kinase, testis specific 2 (cDNA clone MGC:67175 IMAGE:6813507) 10 ug
$1,000.00

Plasmid Map for MR210538

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210538
Circular map for MR210538

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