Arhgap44 (NM_175003) Mouse Tagged ORF Clone

SKU MR210534

Arhgap44 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 44 (Arhgap44), transcript variant 2

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Arhgap44
• Synonyms: 6330543G20; AI840762; AU040829; AW493732; Rich2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210534 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGAAGCAGTTTAACCGCATGCGCCAGTTGGCCAACCAGACGGTGGGCAGGGCAGAAAAGACTGAAG; TTCTGAGCGAAGACCTTCTCCAGGTGGAGAAGCGCCTAGAGCTGGTGAAGCAGGTGTCCCACAGCACACA; CAAGAAGCTCACCGCATGTCTGCAGGGTCAGCAGGGAGCAGAGGCCGACAAGCGATCAAAAAAGTTGCCT; CTGACGACTCTGGCGCAGTGCTTGGTGGAGGGGTCCGCGATCCTGGGCGATGACACGCTTCTTGGGAAGA; TGCTGAAGCTCTGCGGAGAGACGGAGGACAAGCTAGCTCAGGAGCTGATCCACTTTGAGCTGCAAGTGGA; GAGGGATGTGATCGAGCCCCTGTTCCTGCTCGCTGAGGTGGAAATTCCAAATATTCAAAAGCAGAGGAAA; CATTTAGCCAAGCTGGTGCTTGACATGGACTCTTCCCGGACCAGGTGGCAGCAGACTTCCAAGTCTTCAG; GGCTTTCCAGCAGCCTTCAGCCTGCGGGTGCCAAAGCCGATGCCCTCAGGGAGGAGATGGAGGAGGCTGC; CAACAGAGTGGAGATTTGCCGGGACCAGCTTTCTGCTGACATGTACAGTTTTGTGGCCAAAGAAATTGAC; TATGCAAACTACTTTCAAACGCTAATAGAAGTGCAAGCTGAGTACCACAGGAAGTCGCTGACACTGCTGC; AGGCTGTGTTGCCTCAGATCAAAGCCCAGCAGGAGGCCTGGGTGGAGAAGCCTTCCTTTGGGAAGCCCCT; GGAGGAGCACCTGATGATCAGTGGCCGAGAGATCGCCTTCCCTATCGAGGCGTGTGTGACCATGCTGCTG; GAGTGTGGGATGCAGGAGGAGGGCCTCTTCCGGGTAGCCCCCTCAGCCTCCAAGCTGAAGAAGCTGAAAG; CTGCTCTGGACTGCTGTGTGGTGGATGTCCAGGAGTACTCGGCTGACCCCCACGCCATAGCAGGGGCTTT; GAAGTCTTATCTCCGAGAGTTGCCAGAGCCTCTTATGACCTTTGAACTCTATGACGAATGGATCCAGGCT; TCTAATATCCAGGAGCAAGACAAGAGACTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCA; ACCATAACAACATCAAATACTTGATCAAATTTTTATCGAAACTGTCTGAATATCAGGATGTAAACAAGAT; GACTCCCAGTAACATGGCAATTGTCCTGGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACC; GAGATGATGACCACAGTTTCCCTGCAGATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGT; TCTTCCCTGGAGAGATAGAGTTCAACCTCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGC; CAACTACAGCTCAATGCCCTCCCCAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGG; CCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGATGGCATGGGTGTGAGGGTCA; TGGACACATCTTGGGTGGCACGAAGAGGCTCCTCTGCTGGTCGGAAAGCATCCTGCGCCCCGCCTTCCAT; GCAGCCTCCTGCCCCACCCTCCGAGCTGGCTGCACCCTTGCCTTCACCCCTGCCGGAGCAGGTCCCAGAC; AGCCCTGCCACTCCAGCTCCTGCGCTCTCTCCATCAGGTGCCAGCCTGCAGCCCACCCCCGAGCGCCCCA; GTGTGTCTAAAAGCAAGGAACTGTCGCCAGGGTCTGGGCAGAAAGGAAGTCCAGGTTCCATCCAGGGGAC; ACCCTGCCCAGGGACGCAACTGGGGCCACAGCCGGCTGCCAGCCCCAGCCAGCTTCCTGCAGACCAGAGT; CCTCACACCCTCCGGAAAGTTTCAAAGAAGGTGGCACCAATTCCTCCCAAGGTCCCCTTCGTGCAGCCAG; GGACTGTTTCAGATCAGCCCGTTGGCCAGCCATCCCCAGTCAGCCTGTCTCCTACTCCACCAAGCACCCC; ATCCCCCTATGGACTGAGCTACCCTCCAGGCTACTCCATGGCCTCTGGCCAGCTTTCTCCTGCCTCAGCT; CCTCCCCTGGCCTCTCCCTCTGTCTTTACAAGCACTTTAGCCAAATCTCGGCCCACCCCTAAGCCTCGAC; AGAGGCCCACCCTGCCGCCTCCTCAGCCTCCCAGTGTCAGCCTGTCGGCTTCAAGTCCACAGTCCACAGA; GCACCCCATGTTAGACGGCATGTCGCCGGGGGAAAGCATGTCTACAGCTGTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210534 protein sequence; Red=Cloning site Green=Tags(s); ; MKKQFNRMRQLANQTVGRAEKTEVLSEDLLQVEKRLELVKQVSHSTHKKLTACLQGQQGAEADKRSKKLP; LTTLAQCLVEGSAILGDDTLLGKMLKLCGETEDKLAQELIHFELQVERDVIEPLFLLAEVEIPNIQKQRK; HLAKLVLDMDSSRTRWQQTSKSSGLSSSLQPAGAKADALREEMEEAANRVEICRDQLSADMYSFVAKEID; YANYFQTLIEVQAEYHRKSLTLLQAVLPQIKAQQEAWVEKPSFGKPLEEHLMISGREIAFPIEACVTMLL; ECGMQEEGLFRVAPSASKLKKLKAALDCCVVDVQEYSADPHAIAGALKSYLRELPEPLMTFELYDEWIQA; SNIQEQDKRLQALWNACEKLPKANHNNIKYLIKFLSKLSEYQDVNKMTPSNMAIVLGPNLLWPQSEGNIT; EMMTTVSLQIVGIIEPIIQHADWFFPGEIEFNLTGSYGSPVHVNHNANYSSMPSPDMDPADRRQPEQARR; PLSVATDNMMLEFYKKDGMGVRVMDTSWVARRGSSAGRKASCAPPSMQPPAPPSELAAPLPSPLPEQVPD; SPATPAPALSPSGASLQPTPERPSVSKSKELSPGSGQKGSPGSIQGTPCPGTQLGPQPAASPSQLPADQS; PHTLRKVSKKVAPIPPKVPFVQPGTVSDQPVGQPSPVSLSPTPPSTPSPYGLSYPPGYSMASGQLSPASA; PPLASPSVFTSTLAKSRPTPKPRQRPTLPPPQPPSVSLSASSPQSTEHPMLDGMSPGESMSTAV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175003
• ORF Size: 2292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_175003.3, NP_778168.2
• RefSeq Size: 4099 bp
• RefSeq ORF: 2295 bp
• Locus ID: 216831
• UniProt ID: Q5SSM3
• Cytogenetics: 11 B3
• MW: 83.3 kDa
• Summary: GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1 (PubMed:24352656, PubMed:26969129). In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity (PubMed:23739967, PubMed:24352656, PubMed:26969129). Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation (By similarity). In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation (PubMed:23739967).[UniProtKB/Swiss-Prot Function]