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AW551984 (BC061211) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210528
AW551984 (Myc-DDK-tagged) - Mouse expressed sequence AW551984 (cDNA clone MGC:74355 IMAGE:30250574)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $699.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol AW551984
Synonyms 3222402N16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210528 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCGTTCTGGCGGCTTACTCACTAGCATAAGAGATCCAGTGGCACTGAAGAGCATTGCTGTGACTC
TGTCCATCAATGATTTTGTGGCTGGTGTGTCTGCAACCCTAAACTATGAGAATGAAGAGAAATCACCCCT
GGAGGCCTTCTTTGTGTTCCCCATGGATGAAGATTCTGCTGTCTACAGCTTTGAGGCCTTTGTGGATGGG
AAGAAAATTGTGGCAGAACTTCAGGACAAGTACCAGGCCCACAAAAGGTATGAAGAGGCCCTCTCTGGAG
GATATCAGGCCTACTTATTGGAGGAAGACAAGTGTTCCCGGGATGTCTTCTGTTGCAATGTGGGGAACCT
GCAACCAGGGTCAAAAGTGTCACTGACCCTGAGATATGTGCAAGAACTTCCCTTGGAAGACGATGGGGCC
CTGCGTTATGTGCTCCCAGCCATCCTGAATCCTAGATACCATCTCTCCGATGGACGTGAGGACAGTAATC
TGGATATGAAGACCCCAATAGTCCCTTTGGAGGAATTGCCCTACACACTCAGCATGGTTGCCACTATCAG
CTCCCAGCTTGGTATCAGTACAATCCAATCCAACTGTCCCTTGAATCCCATTGAGTACACTGGAGAATTT
GTGTTCCTTATGGACCGTTCAAGAAGCATGAATAGCCCCATGAGTTCCAAGGACAAGTCTCAGCTGAGAA
TAGATGCTGCCAAGGAAACACTGATTCTGCTATTGAAGAGTTTGCCAATGGGCTGCTATTTCAACATCTA
TGGATTTGGAGCCACCCATGAGGAGTTCTTTCCAGACAGTGTGATGTACAATCAGGAAACCATGCAGATT
GCTGTGAAGAAAGTAAAGCGTTTGTTGGCGGATCTAGGAGGGACTGAACTTTTAACACCACTACGGAAAA
TTTTTAGGAAACCCCCAATCCCAGGGCATCCCTTACAGGTCTTTGTCTTCACTGATGGAGAAGTTGTTGA
AACTTTTAGTGTAATCAGAGAAGTTATGTTCCAGAGCAAGAAGCACAGATGTTTCTCATTTGGCATTGGA
GAAGGGGCCTCCACTAGCTTAATCAAGAACCTTGCCCGGGTGTCAGGGGGCACTGCAGAGTTTATCACAG
GCAATGATAGGATGCAGTCCAAGGCCCTTAGATCTCTGAGGCGTTCTTTACAGTCTGTAGTAGAGGATGT
CTCTCTGAGCTGGAATTTGCCTCCCAAGATGTTTGCACCTATGCTATCTCCAGAACAAACTTCTATCTTT
AGGGGTCAAAGGCTAATCATCTACAGCCTACTGATTGGAAAAATACCAAAAGAAGAGGCAACCGGTGAAG
TGTGTCTCTCATATAAGCTTCATGGTAAAAGTTATGAGGAAAAAGTGACATTTTCTCTACAGCCAAAGCC
TGATGACAACCTCACCATTCACCGTCTTGCTGCAAAGTCCATGATCCAGTCCAAAGACTTTGGCTTCAAG
GAGACGTCACTAATGTATAAGAGAGATGTGCTGGACATCAGCCTAGAGTCTGGAGTCATGAGTTCCTTTA
CAGCCTTTGTTGCCATCAACAAGGAGCTCAACGAGCCCATCCAGGGGCCCCTGGTGCATAGGGATATCCC
AAGGCCTATTCTCTTGGGTGCAGGTCCTAATAGGCCCTCTGATGGTGGTTATCCCAAAACTTTATTCTGC
GCCAGTGCTCCTGTAACTCTGCCTCCATGGCTGAAGGGCTCAGGAAAATGTGTTGCTACAATACTCATGC
CAAATGGCACACCCCAACAGCGTCAGAACAGCCAGAAGAAGAAATGTGTGGTTCATACAGATACTCGCCC
TGAGTTGCCCCACCTGGCTAAATATGACAGTTACCCACAAGGGAGTAAGGAGCATAGTATTGCACAGCTG
ATATATCTCCAGAATGCAGATGGTTCCTGGGATATGAATGAGGATTTAGCTAAGATTCTAAGTACTAGTT
TGGAAGACATCACAGCTGCATGCCCTACTGAGGTGTTAGAACCCTCAGCCTGGGCTACAATGCTGGCCGT
GATCTGGCTGCATGCCAATGGCAAGGAATTGAAGGACGAGTGGGAGCTTCTGGAAAGAAAGGCTGTGGTT
TGGCTTCACAATAATTCAGCCCTGACTCTTATTCCTCCTCTCCACAGTCCGTGGTTTCAACAAGCTTATA
CGTACTGCCAACAAGCTCTTGAAGACATCTTTGAGCCCCTCGATCTTCAAGTTATAAGAGTGATGTCCAG
GAGAATGGCATTCTTTCCTGCCTCCTGCCACACCCTTCACCTCATCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210528 protein sequence
Red=Cloning site Green=Tags(s)

MVRSGGLLTSIRDPVALKSIAVTLSINDFVAGVSATLNYENEEKSPLEAFFVFPMDEDSAVYSFEAFVDG
KKIVAELQDKYQAHKRYEEALSGGYQAYLLEEDKCSRDVFCCNVGNLQPGSKVSLTLRYVQELPLEDDGA
LRYVLPAILNPRYHLSDGREDSNLDMKTPIVPLEELPYTLSMVATISSQLGISTIQSNCPLNPIEYTGEF
VFLMDRSRSMNSPMSSKDKSQLRIDAAKETLILLLKSLPMGCYFNIYGFGATHEEFFPDSVMYNQETMQI
AVKKVKRLLADLGGTELLTPLRKIFRKPPIPGHPLQVFVFTDGEVVETFSVIREVMFQSKKHRCFSFGIG
EGASTSLIKNLARVSGGTAEFITGNDRMQSKALRSLRRSLQSVVEDVSLSWNLPPKMFAPMLSPEQTSIF
RGQRLIIYSLLIGKIPKEEATGEVCLSYKLHGKSYEEKVTFSLQPKPDDNLTIHRLAAKSMIQSKDFGFK
ETSLMYKRDVLDISLESGVMSSFTAFVAINKELNEPIQGPLVHRDIPRPILLGAGPNRPSDGGYPKTLFC
ASAPVTLPPWLKGSGKCVATILMPNGTPQQRQNSQKKKCVVHTDTRPELPHLAKYDSYPQGSKEHSIAQL
IYLQNADGSWDMNEDLAKILSTSLEDITAACPTEVLEPSAWATMLAVIWLHANGKELKDEWELLERKAVV
WLHNNSALTLIPPLHSPWFQQAYTYCQQALEDIFEPLDLQVIRVMSRRMAFFPASCHTLHLIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC061211
ORF Size 2289 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC061211, AAH61211
RefSeq Size 3304 bp
RefSeq ORF 2291 bp
Locus ID 244810
Cytogenetics 9 A5.1
MW 85.4 kDa
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"BC061211" in other vectors (4)
SKU Description Size Price
MC220701 AW551984 (untagged) - Mouse expressed sequence AW551984 (cDNA clone MGC:74355 IMAGE:30250574), (10ug) 10 ug
$769.00
MG210528 AW551984 (tGFP-tagged) - Mouse expressed sequence AW551984 (cDNA clone MGC:74355 IMAGE:30250574) 10 ug
$899.00
MR210528L3 Lenti ORF clone of AW551984 (Myc-DDK-tagged) - Mouse expressed sequence AW551984 (cDNA clone MGC:74355 IMAGE:30250574) 10 ug
$999.00
MR210528L4 Lenti ORF clone of AW551984 (mGFP-tagged) - Mouse expressed sequence AW551984 (cDNA clone MGC:74355 IMAGE:30250574) 10 ug
$999.00

Plasmid Map for MR210528

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210528
Circular map for MR210528

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