Mlh1 (NM_026810) Mouse Tagged ORF Clone

SKU MR210511

Mlh1 (Myc-DDK-tagged) - Mouse mutL homolog 1 (E. coli) (Mlh1)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Mlh1
• Synonyms: 1110035C23Rik; AI317206; AI325952; AI561766
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210511 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGTTTGTAGCAGGAGTTATTCGGCGTCTGGACGAGACGGTGGTGAACCGCATAGCGGCGGGGGAAG; TCATTCAGCGGCCGGCCAATGCTATCAAAGAGATGATAGAAAACTGTTTAGATGCAAAATCTACAAATAT; TCAAGTGGTTGTTAAGGAAGGTGGCCTGAAGCTAATTCAGATCCAAGACAATGGCACTGGAATCAGGAAG; GAAGATCTGGATATTGTGTGTGAGAGGTTCACTACGAGTAAACTGCAGACTTTTGAGGATTTAGCCAGTA; TTTCTACCTATGGCTTTCGTGGTGAGGCATTGGCAAGCATAAGCCATGTGGCCCATGTCACTATTACAAC; CAAAACAGCTGATGGGAAATGTGCGTACAGAGCAAGTTACTCAGATGGAAAGCTGCAAGCCCCTCCTAAA; CCCTGTGCAGGCAACCAGGGCACCCTGATCACGGTGGAAGACCTTTTTTACAACATAATCACAAGGAGGA; AAGCTTTAAAAAATCCAAGTGAAGAGTACGGAAAAATTTTGGAAGTTGTTGGCAGGTATTCAATACACAA; TTCAGGCATTAGTATCTCAGTTAAAAAACAAGGTGAGACAGTATCTGATGTCAGAACACTGCCCAATGCC; ACAACCGTGGACAACATTCGCTCCATCTTTGGAAATGCGGTTAGTCGAGAACTGATAGAAGTTGGGTGTG; AGGATAAAACCCTAGCTTTCAAAATGAATGGCTATATATCGAATGCAAACTATTCAGTGAAGAAGTGCAT; TTTCCTACTCTTCATCAACCACCGTCTGGTAGAATCAGCTGCCTTGAGAAAAGCCATTGAAACTGTATAT; GCAGCATATTTGCCCAAAAACACACACCCATTCCTGTACCTCAGTTTGGAAATCAGCCCTCAGAACGTGG; ACGTCAATGTACACCCCACCAAGCACGAAGTTCACTTTCTGCATGAGGAGAGCATTCTGCAGCGTGTGCA; GCAGCACATTGAGAGCAAGCTGCTGGGCTCCAATTCCTCCAGGATGTATTTCACCCAGACCTTGCTTCCA; GGACTTGCTGGGCCCTCTGGGGAGGCAGCTAGACCCACGACAGGGGTGGCTTCCTCATCCACTAGTGGAA; GTGGCGACAAGGTCTACGCTTACCAGATGGTCCGTACGGACTCCCGGGATCAGAAGCTTGACGCCTTTCT; GCAGCCTGTAAGCAGCCTTGTGCCCAGCCAGCCCCAGGACCCTGCCCCTGTCCGAGGGGCCAGGACAGAG; GGCTCTCCTGAAAGGGCCACGCGGGAGGATGAGGAGATGCTTGCTCTCCCAGCCCCCGCTGAAGCAGCTG; CTGAGAGTGAGAACTTGGAGAGGGAATCACTAATGGAGACTTCAGACACAGCCCAGAAAGCGGCACCCAC; TTCCAGTCCAGGAAGCTCCAGAAAGAGACATCGGGAGGACTCTGATGTGGAAATGGTGGAAAATGCTTCC; GGGAAGGAAATGACAGCTGCTTGCTACCCCAGGAGGAGGATCATTAACCTCACCAGCGTCTTGAGTCTCC; AGGAAGAGATTAGTGAGCGGTGCCATGAGACTCTCCGGGAGATGCTCCGTAACCATTCCTTTGTGGGCTG; TGTGAATCCTCAGTGGGCCTTGGCACAGCACCAGACCAAGCTATACCTCCTCAACACTACCAAGCTCAGT; GAAGAGCTGTTCTACCAGATACTCATTTATGATTTTGCCAACTTTGGTGTTCTGAGGTTATCGGAACCAG; CGCCACTCTTCGACCTGGCCATGCTGGCCTTAGACAGTCCTGAAAGTGGCTGGACAGAGGACGACGGCCC; GAAGGAAGGGCTTGCAGAGTACATTGTCGAGTTTCTGAAGAAGAAAGCGGAGATGCTTGCAGACTATTTC; TCTGTGGAGATCGATGAGGAAGGGAACCTGATCGGATTACCTCTTCTGATTGACAGCTATGTGCCACCTT; TGGAGGGACTGCCTATCTTCATTCTTCGACTGGCCACTGAGGTGAATTGGGATGAAGAAAAGGAGTGTTT; TGAAAGTCTCAGTAAAGAATGTGCTATGTTTTACTCCATTCGGAAGCAGTATATACTGGAGGAGTCGACC; CTCTCAGGCCAGCAGAGTGACATGCCTGGCTCCACGTCAAAGCCCTGGAAGTGGACTGTGGAGCACATTA; TCTATAAAGCCTTCCGCTCACACCTCCTACCTCCGAAGCATTTCACAGAAGATGGCAATGTCCTGCAGCT; TGCCAACCTGCCAGATCTATACAAAGTCTTTGAGCGGTGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210511 protein sequence; Red=Cloning site Green=Tags(s); ; MAFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTNIQVVVKEGGLKLIQIQDNGTGIRK; EDLDIVCERFTTSKLQTFEDLASISTYGFRGEALASISHVAHVTITTKTADGKCAYRASYSDGKLQAPPK; PCAGNQGTLITVEDLFYNIITRRKALKNPSEEYGKILEVVGRYSIHNSGISISVKKQGETVSDVRTLPNA; TTVDNIRSIFGNAVSRELIEVGCEDKTLAFKMNGYISNANYSVKKCIFLLFINHRLVESAALRKAIETVY; AAYLPKNTHPFLYLSLEISPQNVDVNVHPTKHEVHFLHEESILQRVQQHIESKLLGSNSSRMYFTQTLLP; GLAGPSGEAARPTTGVASSSTSGSGDKVYAYQMVRTDSRDQKLDAFLQPVSSLVPSQPQDPAPVRGARTE; GSPERATREDEEMLALPAPAEAAAESENLERESLMETSDTAQKAAPTSSPGSSRKRHREDSDVEMVENAS; GKEMTAACYPRRRIINLTSVLSLQEEISERCHETLREMLRNHSFVGCVNPQWALAQHQTKLYLLNTTKLS; EELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEDDGPKEGLAEYIVEFLKKKAEMLADYF; SVEIDEEGNLIGLPLLIDSYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYILEEST; LSGQQSDMPGSTSKPWKWTVEHIIYKAFRSHLLPPKHFTEDGNVLQLANLPDLYKVFERC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026810
• ORF Size: 2280 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_026810.1, NP_081086.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 2283 bp
• Locus ID: 17350
• UniProt ID: Q9JK91
• Cytogenetics: 9 60.92 cM
• MW: 84.7 kDa
• Summary: Heterodimerizes with Pms2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (Msh2-Msh6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of Pms2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (Mlh1-Pms2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with Mlh3 to form MutL gamma which plays a role in meiosis (By similarity).[UniProtKB/Swiss-Prot Function]