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Heg1 (NM_175256) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210481
Heg1 (Myc-DDK-tagged) - Mouse HEG homolog 1 (zebrafish) (Heg1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $691.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Heg1
Synonyms 4632417D23Rik; 5530401I02Rik; 9530025L16Rik; BC038614; Gm629; OTTMUSG00000027315
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210481 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTCCGACGATGACGAGCCTGCTCAGTCTTCCACAGAGTCACCGGTTCTGCATACGTCCAACCTGC
CAACCTACACGTCCACTGTTAATATGCCAAACACCTTGGTTCTTGATACCGGTACCAAACCTGTTGAGGA
CCCATCTGATTCACGGGTGCCTTCCACTCAACCCTCACCGTCACAACCCCAGCCATTCTCATCAGCCTTA
CCATCAACCAGGTCTCCTGGGTCTACCTCTGAGACAACCACATCCTCACCATCACCTTCACCCATCTCCT
TACTGGTGTCAACTCTTGCCCCATATTCTGTCTCACAAACAACCTTCCCACATCCATCTTCTACCCTAGT
CCCTCACAGGCCAAGGGAGCCACGTGTGACTTCAGTCCAAATGTCGACGGCAATATCAGCCATTGCATTG
ATCCCCAGCAATCAAACAGCAAACCCCAAGAACCAGAGTACCCCACAACAAGAGAAACCCATCACAGAAG
CTAAGTCACCAAGCTTGGTGTCTCCGCCCACAGACTCTACCAAAGCAGTAACAGTGAGCCTTCCTCCAGG
GGCCCCTTGGTCCCCAGCCTTAACAGGGTTCTCCACGGGGCCAGCCCTTCCAGCCACAAGCACCAGTTTA
GCCCAGATGTCTCCAGCTTTGACATCTGCCATGCCCCAGACGACTCACTCTCCTGTGACCAGTCCCAGTA
CTCTGTCACATGTAGAAGCTTTGACTTCAGGTGCTGTAGTTGTGCACACTACCCCGAAGAAACCACATCT
CCCAACCAATCCTGAAATCCTAGTGCCACACATCTCAACTGAGGGGGCCATCACCACAGAAGGGAACCGG
GAGCATACGGATCCTACCACTCAGCCAATCCCTCTGACCACTTCAACCACGTCAGCAGGAGAAAGGACCA
CAGAGCTGGGCCGTGCGGAAGAGTCCAGTCCATCCCATTTTCTTACACCATCTTCACCTCAAACCACAGA
TGTTTCTACAGCTGAAATGTTGACTTCCAGATATATCACCTTTGCTGCTCAGAGCACCTCACAGTCACCA
ACAGCCTTGCCACCTCTAACCCCAGTCAACAGCTGTACCGTGAACCCTTGTCTTCATGATGGCAAATGCA
TTGTGGACCTCACTGGCCGTGGGTATCGATGTGTGTGCCCGCCTGCCTGGCAGGGGGAGAACTGCAGTGT
GGATGTGAACGAATGTCTCTCCAGCCCCTGTCCTCCCCTAGCCACGTGCAACAATACTCAGGGATCGTTC
ACCTGCAGATGCCCAGTCGGGTATCAGTTGGAGAAAGGAATATGCAATCTGGTTAGAACCTTTGTGACCG
AGTTTAAGTTAAAGAAGACTTTTCTCAATACTACTGCAGAAAACCATTCCAACACTCAAGAGCTTGAAAA
CGAGATCGCTCAAACACTAAACGTGTGTTTTTCAACATTGCCCGGTTATATCCGAACTACAGCCCATGTG
TCTAGGGAGCCCAGTACAGTTTTCATCTCACTGAAAACCACCTTTGCCCTGGCCTCCAATGTGACACTGT
TTGACCTGGCAGACAGGATCCAGAAATATGTCAACTCCTGCAGGTCCTCTGCTGAAGTCTGCCAGCTCTT
GGGGTCTCAGAGGCGGGTCTTTCGAGCGGGCAGCTTGTGTAAGCGGAAGAGTCCAGAATGTGACAAAGAG
ACCTCCATCTGCACCGACCTGGATGGTGTCGCGCTCTGTCAGTGCAAGTCTGGCTACTTCCAGTTCAACA
AGATGGATCACTCTTGCCGAGCATGTGAAGATGGATATAGGCTTGAAAATGAAACCTGTATGAGTTGCCC
ATTTGGCCTCGGTGGACTCAACTGTGGAAACCCCTATCAGCTCATCACTGTGGTGATCGCAGCCGCAGGA
GGTGGCCTCTTGCTCATCCTGGGCGTGGCACTCATTGTTACCTGTTGCAGAAAGAGTAAAAATGACATAA
GTAAACTCATCTTCAAAAGTGGGGACTTCCAAATGTCCCCATACACCGATGTCCCCAAGAATCCTCGCTC
GCAAGAGTGGGGCCGAGAAGCCATTGAAATGCACGAGAACGGAAGCACCAAAAACCTCCTGCAGATGACA
GACGTGTACTACTCGCCCACAAACGTAAGGAACCCTGAACTTGAACGCAACGGACTGTACCCAGCCTACA
CTGGACTGCCCGGATCGAGACACTCTTGCATCTTCCCGGGACAGTATAACCCATCTTTCATCAGCGACGA
GAGCAGGAGGAGAGACTACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210481 protein sequence
Red=Cloning site Green=Tags(s)

MSSDDDEPAQSSTESPVLHTSNLPTYTSTVNMPNTLVLDTGTKPVEDPSDSRVPSTQPSPSQPQPFSSAL
PSTRSPGSTSETTTSSPSPSPISLLVSTLAPYSVSQTTFPHPSSTLVPHRPREPRVTSVQMSTAISAIAL
IPSNQTANPKNQSTPQQEKPITEAKSPSLVSPPTDSTKAVTVSLPPGAPWSPALTGFSTGPALPATSTSL
AQMSPALTSAMPQTTHSPVTSPSTLSHVEALTSGAVVVHTTPKKPHLPTNPEILVPHISTEGAITTEGNR
EHTDPTTQPIPLTTSTTSAGERTTELGRAEESSPSHFLTPSSPQTTDVSTAEMLTSRYITFAAQSTSQSP
TALPPLTPVNSCTVNPCLHDGKCIVDLTGRGYRCVCPPAWQGENCSVDVNECLSSPCPPLATCNNTQGSF
TCRCPVGYQLEKGICNLVRTFVTEFKLKKTFLNTTAENHSNTQELENEIAQTLNVCFSTLPGYIRTTAHV
SREPSTVFISLKTTFALASNVTLFDLADRIQKYVNSCRSSAEVCQLLGSQRRVFRAGSLCKRKSPECDKE
TSICTDLDGVALCQCKSGYFQFNKMDHSCRACEDGYRLENETCMSCPFGLGGLNCGNPYQLITVVIAAAG
GGLLLILGVALIVTCCRKSKNDISKLIFKSGDFQMSPYTDVPKNPRSQEWGREAIEMHENGSTKNLLQMT
DVYYSPTNVRNPELERNGLYPAYTGLPGSRHSCIFPGQYNPSFISDESRRRDYF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175256
ORF Size 2262 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175256.4, NP_780465.3
RefSeq Size 4804 bp
RefSeq ORF 3249 bp
Locus ID 77446
Cytogenetics 16 B3
MW 81.1 kDa
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"NM_175256" in other vectors (4)
SKU Description Size Price
MC203436 Heg1 (untagged) - Mouse HEG homolog 1 (zebrafish) (Heg1), (10ug) 10 ug
$691.00
MG210481 Heg1 (tGFP-tagged) - Mouse HEG homolog 1 (zebrafish) (Heg1) 10 ug
$891.00
MR210481L3 Lenti ORF clone of Heg1 (Myc-DDK-tagged) - Mouse HEG homolog 1 (zebrafish) (Heg1) 10 ug
$991.00
MR210481L4 Lenti ORF clone of Heg1 (mGFP-tagged) - Mouse HEG homolog 1 (zebrafish) (Heg1) 10 ug
$991.00

Plasmid Map for MR210481

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210481
Circular map for MR210481

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