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Pla2g4a (NM_008869) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210449
Pla2g4a (Myc-DDK-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $685.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pla2g4a
Synonyms cP; cPL; cPLA2; cPLA2-alpha; cPLA2alpha; Pla; Pla2g4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210449 representing NM_008869
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTCATAGATCCTTATCAGCACATTATAGTGGAACACCAGTACTCCCATAAGTTTACTGTTGTGG
TTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGACACTCCAGATCCTTATGTGGA
ACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACGAGACACTTCAATAATGATATAAACCCCGTG
TGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATCACACTGATGGATG
CCAACTACGTCATGGATGAAACCCTAGGCACAGCTACATTCCCTGTATCTTCAATGAAAGTGGGAGAGAA
GAAAGAAGTCCCTTTTATTTTCAACCAAGTCACTGAAATGATTCTGGAAATGTCTCTGGAAGTTTGTTCA
TGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACTTTCAGACAGCAGAGGAAAGAGA
ACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGTGAGGGGCTTTATTCCACACGTGATGT
GCCGGTGGTGGCCATTTTGGGTTCAGGTGGGGGTTTCCGGGCCATGGTGGGATTCTCTGGTGTGATGAAG
GCACTGTATGAGTCGGGGATTTTGGACTGTGCTACATACATTGCTGGTCTTTCTGGATCCACATGGTACA
TGTCAACCTTGTACTCTCACCCCGATTTTCCAGAGAAAGGTCCCGAGGAGATTAATGAAGAGCTAATGAA
AAATGTCAGCCACAACCCTCTCTTACTTCTTACACCACAGAAAGTTAAAAGATACGTTGAGTCTTTATGG
AAGAAGAAAAGTTCTGGCCAGCCTGTCACCTTTACTGACATCTTTGGGATGCTAATAGGAGAAACACTAA
TTCAAAATAGGATGAGCATGACCCTGAGTAGTTTGAAGGAAAAGGTCAATGCCGCCCGGTGTCCTTTGCC
TCTCTTCACGTGTCTCCACGTCAAACCTGATGTGTCAGAGCTGATGTTTGCCGATTGGGTGGAATTTAGT
CCATATGAGATTGGCATGGCAAAATATGGTACCTTTATGGCTCCTGACCTATTTGGAAGCAAGTTTTTTA
TGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTGATGGGTGTCTGGGGCAGTGCCTT
TTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAGGCTCTACAATGGAAGAGGAA
TTAGAAAATATTACAGCAAAGCACATCGTGAGTAATGACAGCTCCGACAGTGATGATGAGGCTCAAGGAC
CCAAAGGCACCGAGAATGAAGAAGCTGAAAAAGAGTACCAAAGCGACAACCAAGCAAGTTGGGTCCATCG
GATGCTAATGGCCTTGGTGAGCGACTCGGCTTTATTCAATACCCGAGAAGGACGTGCCGGAAAGGTGCAT
AACTTCATGCTGGGCTTGAATCTCAACACATCATATCCACTGTCTCCCCTGAGAGACTTCAGCTCTCAGG
ATTCCTTCGATGACGAGCTCGACGCAGCGGTAGCAGATCCAGATGAATTTGAACGAATATATGAACCACT
GGATGTCAAAAGTAAGAAGATTCATGTGGTAGATAGTGGGCTCACATTTAACCTGCCATATCCCTTGATT
CTTCGACCTCAGAGAGGTGTGGATCTTATCATCTCCTTTGACTTTTCTGCAAGGCCGAGTGACACCAGTC
CCCCTTTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCGAAAATGAACAAGCTTCCCTTTCCAAAGATCGA
TCCTTATGTGTTTGATCGGGAAGGATTAAAGGAATGCTATGTTTTTAAACCTAAGAATCCTGATGTGGAG
AAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCCCCAGGTG
TTCTAAGGGAAACCAAAGAAGAGAAAGAAATTGCTGACTTTGACATTTTTGATGACCCCGAATCGCCATT
TTCAACCTTCAACTTTCAGTATCCCAATCAAGCATTCAAAAGGCTTCACGATTTGATGTACTTCAACACA
CTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATACAGAAGACAGAACCCATCTC
GTTGCTCTGTTTCCCTCAGTAATGTTGAAGCAAGAAAATTCTTCAATAAGGAGTTTCTAAGTAAACCCAC
TGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210449 representing NM_008869
Red=Cloning site Green=Tags(s)

MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGTFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPV
WNETFEFILDPNQENVLEITLMDANYVMDETLGTATFPVSSMKVGEKKEVPFIFNQVTEMILEMSLEVCS
CPDLRFSMALCDQEKTFRQQRKENIKENMKKLLGPKKSEGLYSTRDVPVVAILGSGGGFRAMVGFSGVMK
ALYESGILDCATYIAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLW
KKKSSGQPVTFTDIFGMLIGETLIQNRMSMTLSSLKEKVNAARCPLPLFTCLHVKPDVSELMFADWVEFS
PYEIGMAKYGTFMAPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQNKGSTMEEE
LENITAKHIVSNDSSDSDDEAQGPKGTENEEAEKEYQSDNQASWVHRMLMALVSDSALFNTREGRAGKVH
NFMLGLNLNTSYPLSPLRDFSSQDSFDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPLI
LRPQRGVDLIISFDFSARPSDTSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDVE
KDCPTIIHFVLANINFRKYKAPGVLRETKEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMYFNT
LNNIDVIKDAIVESIEYRRQNPSRCSVSLSNVEARKFFNKEFLSKPTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008869
ORF Size 2244 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008869.4
RefSeq Size 2846 bp
RefSeq ORF 2247 bp
Locus ID 18783
UniProt ID P47713
Cytogenetics 1 63.51 cM
MW 85.7 kDa
Summary The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Mar 2015
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Documents
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"NM_008869" in other vectors (4)
SKU Description Size Price
MC200328 Pla2g4a (untagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), (10ug) 10 ug
$686.00
MG210449 Pla2g4a (tGFP-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a) 10 ug
$885.00
MR210449L3 Lenti ORF clone of Pla2g4a (Myc-DDK-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a) 10 ug
$985.00
MR210449L4 Lenti ORF clone of Pla2g4a (mGFP-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a) 10 ug
$985.00

Plasmid Map for MR210449

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210449
Circular map for MR210449

file-alt Citations

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