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Pla2g4a (NM_008869) Mouse Untagged Clone

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SKU
MC200328
Pla2g4a (untagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pla2g4a
Synonyms cP; cPL; cPLA2; cPLA2-alpha; cPLA2alpha; Pla; Pla2g4
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC003816 sequence for NM_008869
GAAGACTTTCTAGAAGTCGGAACTGTGAAGGGCTCCCGACTGAGAGCCAGAAGGCACAGAGAAGCCTGAG GATTCTCATTTAACTCTGGGAACTGCTTCAAGAAGCTACAGTACCATAGAAGACCTGGGAAGTGTGAGAA TTTCTGCAACTGGGACCAAAATGTCTTTCATAGATCCTTATCAGCACATTATAGTGGAACACCAGTACTC CCATAAGTTTACTGTTGTGGTTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGAC ACTCCAGATCCTTATGTGGAACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACGAGACACTTCA ATAATGATATAAACCCCGTGTGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTT GGAGATCACACTGATGGATGCCAACTACGTCATGGATGAAACCCTAGGCACAGCTACATTCCCTGTATCT TCAATGAAAGTGGGAGAGAAGAAAGAAGTCCCTTTTATTTTCAACCAAGTCACTGAAATGATTCTGGAAA TGTCTCTGGAAGTTTGTTCATGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACTTT CAGACAGCAGAGGAAAGAGAACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGTGAGGGG CTTTATTCCACACGTGATGTGCCGGTGGTGGCCATTTTGGGTTCAGGTGGGGGTTTCCGGGCCATGGTGG GATTCTCTGGTGTGATGAAGGCACTGTATGAGTCGGGGATTTTGGACTGTGCTACATACATTGCTGGTCT TTCTGGATCCACATGGTACATGTCAACCTTGTACTCTCACCCCGATTTTCCAGAGAAAGGTCCCGAGGAG ATTAATGAAGAGCTAATGAAAAATGTCAGCCACAACCCTCTCTTACTTCTTACACCACAGAAAGTTAAAA GATACGTTGAGTCTTTATGGAAGAAGAAAAGTTCTGGCCAGCCTGTCACCTTTACTGACATCTTTGGGAT GCTAATAGGAGAAACACTAATTCAAAATAGGATGAGCATGACCCTGAGTAGTTTGAAGGAAAAGGTCAAT GCCGCCCGGTGTCCTTTGCCTCTCTTCACGTGTCTCCACGTCAAACCTGATGTGTCAGAGCTGATGTTTG CCGATTGGGTGGAATTTAGTCCATATGAGATTGGCATGGCAAAATATGGTACCTTTATGGCTCCTGACCT ATTTGGAAGCAAGTTTTTTATGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTGATG GGTGTCTGGGGCAGTGCCTTTTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAG GCTCTACAATGGAAGAGGAATTAGAAAATATTACAGCAAAGCACATCGTGAGTAATGACAGCTCCGACAG TGATGATGAGGCTCAAGGACCCAAAGGCACCGAGAATGAAGAAGCTGAAAAAGAGTACCAAAGCGACAAC CAAGCAAGTTGGGTCCATCGGATGCTAATGGCCTTGGTGAGCGACTCGGCTTTATTCAATACCCGAGAAG GACGTGCCGGAAAGGTGCATAACTTCATGCTGGGCTTGAATCTCAACACATCATATCCACTGTCTCCCCT GAGAGACTTCAGCTCTCAGGATTCCTTCGATGACGAGCTCGACGCAGCGGTAGCAGATCCAGATGAATTT GAACGAATATATGAACCACTGGATGTCAAAAGTAAGAAGATTCATGTGGTAGATAGTGGGCTCACATTTA ACCTGCCGTATCCCTTGATTCTGCGACCTCAGAGAGGTGTGGATCTTATCATCTCCTTTGACTTTTCTGC AAGGCCGAGTGACACCAGTCCCCCTTTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCGAAAATGAACAAG CTTCCCTTTCCAAAGATCGATCCTTATGTGTTTGATCGGGAAGGATTAAAGGAATGCTATGTTTTTAAAC CTAAGAATCCTGATGTGGAGAAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAG AAAGTACAAGGCCCCAGGTGTTCTAAGGGAAACCAAAGAAGAGAAAGAAATCGCTGACTTTGACATTTTT GATGACCCCGAATCGCCATTTTCAACCTTCAACTTTCAGTATCCCAATCAAGCATTCAAAAGGCTGCATG ATCTGATGTACTTCAACACACTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATA CAGAAGACAGAACCCATCTCGTTGCTCTGTTTCCCTCAGTAATGTTGAAGCAAGAAAATTCTTCAATAAG GAGTTTCTAAGTAAACCCACTGTGTAATTTCTGTGCTGGGATGATCAAGCCATTTGAATTCCATGACAAT TTGAGTTCAGAACACATTAGAGGTCATCTTACTATGCAGAAGAGACTGGCTGCTACTCAGAGTTGTGGAG ATTTAGCCATGTGTTAGGTGAAAATGATGTTGATTATGTAATACTTAGCAACAGTTTCTGACAGTATGAA TTTTTTGACATTAGCATAGAGCTATATACTGTATTTTAAACATTCCTCACATTTTTTACCTGTACTTTTT ATATAAATATGACATGTCTTTTCTTTTGAAAATATTTAATAGTTTAACTCAGTAAAAGAGACTTCCCATT GTGTGTGAATGTTATTCCGAACTAGATTTGTTCATGCCATGTTACAACACTATTTTTATTTAAATGTTTA TATTTACACATACGAAATAAATACTTTGCTGTACAAATTACCACTAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_008869
Insert Size 2247 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC003816, AAH03816
RefSeq Size 2861 bp
RefSeq ORF 2247 bp
Locus ID 18783
UniProt ID P47713
Cytogenetics 1 63.51 cM
Summary The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Mar 2015
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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"NM_008869" in other vectors (4)
SKU Description Size Price
MG210449 Pla2g4a (tGFP-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a) 10 ug
$885.00
MR210449 Pla2g4a (Myc-DDK-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a) 10 ug
$589.00 $685.00
MR210449L3 Lenti ORF clone of Pla2g4a (Myc-DDK-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a) 10 ug
$985.00
MR210449L4 Lenti ORF clone of Pla2g4a (mGFP-tagged) - Mouse phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a) 10 ug
$985.00

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