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Cnot10 (NM_153585) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210418
Cnot10 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $681.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cnot10
Synonyms 2600001P13Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210418 representing NM_153585
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCAGACAAGCCTGCAGATCAGGGAGCAGAGAAGCATGAAGGGGCAGGCCAATCATCTGGGGTCA
CGGACCAGGAGAAGGAGCTGTCCGCCAGTGCCCTCCAGGCCTTCACGTCTGGAAATTATGATGCCTGTCT
GCAACACCTTGCCTGTCTCCAAGATATAAACAAAGATGATTACAAAATTATTCTGAATACAGCAGTAGCT
GAATTTTTTAAAAATAATCAAACAACAACAGATAATTTGAGACAAACACTTAACCAGCTGAAGAATCAGG
TCCACTCGGCTGTTGAAGAGATGGATGGGTTAGACGATGTTGAGAACAGCATGCTGTATTATAACCAGGC
TGTCATTCTCTATCATCTGCGCCAGTACACGGAGGCCATATCTGTCGGCGAGAAGCTCTATCAGTTCATA
GAACCTTTCGAAGAAAAATTTGCCCAAGCGGTGTGCTTTTTGCTCGTAGACTTGTATATATTAACCCACC
AGGCTGAGAAAGCTCTGCATCTTCTTGCCGTCCTAGAAAAAATGATTTCACAGGGTAGCGGTGGCAAAAA
TGGGAAGAACGAGACTGGCAATAACAGCAGCAAAGATGGATCCAACCCTAAGGCTGAGAGTGCAGCTCTA
ATAGAGGCTGCCAAGTCCAAGATACACCAGTACAAAGTCAGAGGGTACATCCAGATGAAGTCCCTGAAGG
CTTGCAAAAGGGAAATCAAGTCTGTCATGAACACAGCTGGAAATTCTGCACCTTCCCTGTTTCTTAAAAG
CAACTTTGAGTACTTAAGGGGCAACTATCGAAAAGCGGTGAAGCTGTTGAATAGTTCAAACATTGCCGAG
CATCCAGGCTTCATGAAGACAGGTGAATGCTTGAGATGCATGTTCTGGAATAACCTTGGTTGTATCCATT
TTGCCATGAGCAAGCACAATTTGGGGATTTTCTACTTCAAGAAGGCTCTGCAGGAAAATGACAACGTCTG
CGCCCAGCTCAGTGCGGGTGGCACTGATCCAGGCAAAAAGTTTTCTGGAAGACCCATGTGTACATTGCTG
ACCAACAAAAGATATGAGCTGCTCTACAACTGTGGGATCCAGCTGCTGCACGTCGGGAGGCCTCTCGCTG
CCTTCGAGTGTCTGATTGAAGCTGTTCAGGTGTACCACGCGAATCCTCGTCTCTGGCTACGGCTGGCTGA
GTGCTGCATTGCTGCCAATAAAGGGACTTCTGAGCAAGAAACTAAAGGTCTTCCCACCAAAAAAGGCATT
GTACAGTCTATTGTTGGTCAGGGATATCACCGTAAAATAGTTCTGGCATCACAGTCAATTCAGAACACTG
TCTACAATGATGGTCAGTCTTCAGCCATTCCTGTAGCCAGTGTGGAGTTTGCAGCCATCTGTCTCAGAAA
TGCCTTGTTGCTGTTACCTGAAGAACAGCAAGATCCCAAGCAGGAAAATGGCTCCAAGAGCAGCAGCCAG
CTTGGAGGGAACACGGAAAGCAGCGAGAGCAGCGAGACGTGCAGCAGTAAGAGCCATGATGGAGATAAGT
TCATTCCCGCTCCACCGTCTTCTCCGCTGAGGAAACAGGAGTTAGAAAACCTCAAGTGCTCCATCCTTGC
TTGCAGTGCTTATGTGGCTCTGGCCTTAGGTGATAACCTCATGGCTTTGAACCATGCAGATCAGCTCCTG
CAGCAGCCCAAGCTGTCTGGCTCTCTTAAATTTTTGGGCCATTTATATGCCGCAGAAGCCCTCATCTCCC
TCGACAGGATATCTGATGCCATTACACACTTGAACCCAGAGAATGTCACTGATGTCTCCTTAGGGATCTC
TTCAAATGAGCAGGACCAAGGATCAGACAAGGGTGAAAATGAAGCAATGGAATCCTCTGGCAAGCGCGCC
CCTCAGTGCTACCCCAGTTCTGTGAACTCTGCCCGGACTGTGATGCTCTTCAACCTGGGCAGTGCCTACT
GCCTGAGGAGCGAGTACGACAAGGCCCGAAAGTGTCTGCATCAGGCGGCATCGATGATCCATCCTAAGGA
GGTGCCCCCTGAAGCCATCCTGCTGGCTGTCTACCTTGAGCTGCAGAATGGTAATACACAGCTGGCCTTG
CAGATGATCAAGCGGAACCAGCTGCTCCCCGCAGTAAAGGCGCACTCTGATGTGAGGAAGAAGACAGTGT
TCCAGCCCGTGCACCCCATCCAGCCCATCCAAATGCCAGCGTTCACCACCGTGCAGAGGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210418 representing NM_153585
Red=Cloning site Green=Tags(s)

MAADKPADQGAEKHEGAGQSSGVTDQEKELSASALQAFTSGNYDACLQHLACLQDINKDDYKIILNTAVA
EFFKNNQTTTDNLRQTLNQLKNQVHSAVEEMDGLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFI
EPFEEKFAQAVCFLLVDLYILTHQAEKALHLLAVLEKMISQGSGGKNGKNETGNNSSKDGSNPKAESAAL
IEAAKSKIHQYKVRGYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAE
HPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGGTDPGKKFSGRPMCTLL
TNKRYELLYNCGIQLLHVGRPLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPTKKGI
VQSIVGQGYHRKIVLASQSIQNTVYNDGQSSAIPVASVEFAAICLRNALLLLPEEQQDPKQENGSKSSSQ
LGGNTESSESSETCSSKSHDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADQLL
QQPKLSGSLKFLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRA
PQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLAL
QMIKRNQLLPAVKAHSDVRKKTVFQPVHPIQPIQMPAFTTVQRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153585
ORF Size 2232 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153585.5, NP_705813.2
RefSeq Size 2990 bp
RefSeq ORF 2235 bp
Locus ID 78893
UniProt ID Q8BH15
Cytogenetics 9 F3
MW 82.3 kDa
Summary Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153585" in other vectors (4)
SKU Description Size Price
MC206010 Cnot10 (untagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10), (10ug) 10 ug
$682.00
MG210418 Cnot10 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10) 10 ug
$881.00
MR210418L3 Lenti ORF clone of Cnot10 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10) 10 ug
$981.00
MR210418L4 Lenti ORF clone of Cnot10 (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 10 (Cnot10) 10 ug
$981.00

Plasmid Map for MR210418

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210418
Circular map for MR210418

file-alt Citations

Characterizing the interactions between murine cytomegalovirus M72 and the carbon catabolite repression 4-negative on TATA-less (CCR4-NOT) complex
Brahms, Gopal, Xia et al
Virology (2026) 616, 110791 DOI: 10.1016/j.virol.2026.110791
AI Summary: OriGene products, specifically the pCMV6-Entry Myc-DDK-tagged CNOT4 (MR210197), CNOT9 (MR204171), and CNOT10 (MR210418) ORF plasmids, were used to express CCR4-NOT complex subunits, enabling the study of their interactions with M72 in the characterization of murine cytomegalovirus pathogenesis.
Snippet: Despite being an important human pathogen, many Human cytomegalovirus (HCMV) gene products remain uncharacterized. The betaherpesvirus conserved core genes,;pCMV6-Entry Myc-DDK-tagged CNOT4 (OriGene ORF plasmid MR210197), CNOT9 (Rqcd1, OriGene ORF plasmid, MR204171), and CNOT10 (OriGene ORF plasmid, MR210418) amplicons were cloned into a pT7-EGFP-C1 expression
Showing 1-1 of 1 papers.
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