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Tmem2 (BC019745) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210404
Tmem2 (Myc-DDK-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $679.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210404 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTGATGGAGTGTTTGGAAACTACGTCCCTGTGCCTACCACAGATTGCATGGCTGTTTCAACTTTCT
GGATTGCCCACCCCAACAACCACCTGATCAATAACGCAGCTGCAGGCTCACAGGATGCTGGAATATGGTA
TTTATTCCACAAGGAACCAACTGGGGAGTCCAGTGGGCTGCAGCTCCTGGAGAAGCCAGAACTCACTCCG
CTGGGGATATTTTATAACAACAGGGTTCATTCAAATTTTAAGGCTGGTTTATTTGTGGACAAAGGTGTCA
AAACGACCAACGCCAGTGCATCTGACCCAAGGGAGTACCTCTGCCTGGATAACAGTGCAAGGTTTCGGCC
CCATCAGGATGCAGACCCTGAGAAGCCACGCGTTGCCGCCATCATTGACAGGCTCATTGCTTTTAAGAAC
AATGACAATGGAGCCTGGGTCCGAGGAGGAGATATTATTGTTCAGAATTCAGCATTTGCAGATAACGGAA
AGGGGCTGACCTTTGCCAGTGATGGGAGCTTCCCAAGCGATGAAGGGTCCAGTCAGGAGGTGACGGAGTC
CCTCTTTGTCGGGGAGAGTAGGAATTATGGCTTTCAAGGTGGCCAAAACAAGTACATGGGCACAGGGGGA
ATCGATCAGAAGCCTCGGACGCTACCCAGGAACAGGACATTCCCAATCCGAGGCTTTCAGATTTACGATG
GGCCTATTCATCTCACAAAGAGTACTTTCAAAAAGTATGTGCCAACTCCAGACAGGTACAGCAGTGCAAT
TGGCTTCCTCATGAAGAATTCCTGGCAGACAACACCCAGGAATAACGTTTCCCTTGTGAAGTTTGGTCCA
CAGGTCTCTCTGAACGTCTTCTTTGGAAAGCCTGGCCCCTGGTTTGAGGATTGTGAGCTGGACGGCGATA
AGAACTCCATCTTCCATGACATTGATGGCTCTGTGACAGGATACAAGGACACCTATGTGGGAAGGATGGA
CAACTACCTGATCCGCCATCCAAACTGTGTCAATGTTACTAAGTGGAATGCAGTGATCTGTAGCGGGACC
TACGCCCAGGTGTATGTTCAGACCTGGAACACTCCGAATCTTAGCATGATCATCACACGGGATGAATACC
CATCCCACCCAATGGTGCTTCGGGGTATCAACCAGAGGGCCATCTCTCCACAGTACCAGCCTGTCGTCAT
GCTGGAGAAGGGCTACACCATCCACTGGAATGGTCCAGCGCCACGAACCACTTTCTTGTACCTCGTGAAC
TTCAACAAGGATGACTGGATCCGGGTTGGCCTCTGTTACCCAGCCAACACGAGCTTTCAGGTTACCGTTG
GCTTTCTGCAGCGGCAGAACGGCTCATTATCTAGAATTGAGGACTATGAGCCTGCGCGGTCGATGGAGGA
ACTGCAGAAGAAGCCGTCTGAGAGAAAATTCTATTTTGACTCTGGCACAGGGTTATTGTTTCTGTATCTC
AGAGCCCACAGCCACAGAGATGGCCACAGTTACTGTTCATCTCAGGGATGTGAAAGAGTCAAGATTCAGG
CAGCAACAGACTCCAAAGATATCAGTAACTGCATGGCCAAAGCTTACCCGCAGTATTACAAGAAGCCCTC
GGCGGTCAAGCGCATGCCGGCCATGCTCACGGGGCTCTGTCAAGGCTGTGGAACCCACCAGATGGTGTTT
ACCAGTGACCCTCACAAAAGCTACCTCCCTGTACGATTCCAGTCACCTGGGAAAGCAGAAATTCAGCGAG
GAGATCCATCCATTATTTCCGTCAATGGTACTGACTTTACCTTCCGGAGTGCGGGTGTCCTCCTCCTCGT
TGTGGATGCCTGCAGTGTGCCTTTCCGGGTGAAGGAAAAGCGGATGTTCCTTTCTGCAGACGTCAGTCAC
ATGGAAGAATATTTCAAGGCTAGCATCCCCCCAAGGTCAATCGTTCTGTTGAGCACAAGAGGAGAAATTA
AGCAGCTGAATATCTCGGATTCATTAGCACTTCTGGGATTGGCCAAGCCAGCACATCTGTACAGCAAAGG
TAGTGTCGTATTTTTGGGATTCAGCGGAAACTTCGCACCGTCATGGACGAAGCTATTCACCAGTCCCAAT
GAGCAGGGCCTCGGAGTGCTTGAGCAGTTTCTCCCGTTGCAGATGGAGGAATATGGTTGCTCCCGGACCG
GCAGTGTCCACAGAAGAGACCTTGACCTGTTACAGCAGGCTTTGAAAGTGCTT

ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210404 protein sequence
Red=Cloning site Green=Tags(s)

MRDGVFGNYVPVPTTDCMAVSTFWIAHPNNHLINNAAAGSQDAGIWYLFHKEPTGESSGLQLLEKPELTP
LGIFYNNRVHSNFKAGLFVDKGVKTTNASASDPREYLCLDNSARFRPHQDADPEKPRVAAIIDRLIAFKN
NDNGAWVRGGDIIVQNSAFADNGKGLTFASDGSFPSDEGSSQEVTESLFVGESRNYGFQGGQNKYMGTGG
IDQKPRTLPRNRTFPIRGFQIYDGPIHLTKSTFKKYVPTPDRYSSAIGFLMKNSWQTTPRNNVSLVKFGP
QVSLNVFFGKPGPWFEDCELDGDKNSIFHDIDGSVTGYKDTYVGRMDNYLIRHPNCVNVTKWNAVICSGT
YAQVYVQTWNTPNLSMIITRDEYPSHPMVLRGINQRAISPQYQPVVMLEKGYTIHWNGPAPRTTFLYLVN
FNKDDWIRVGLCYPANTSFQVTVGFLQRQNGSLSRIEDYEPARSMEELQKKPSERKFYFDSGTGLLFLYL
RAHSHRDGHSYCSSQGCERVKIQAATDSKDISNCMAKAYPQYYKKPSAVKRMPAMLTGLCQGCGTHQMVF
TSDPHKSYLPVRFQSPGKAEIQRGDPSIISVNGTDFTFRSAGVLLLVVDACSVPFRVKEKRMFLSADVSH
MEEYFKASIPPRSIVLLSTRGEIKQLNISDSLALLGLAKPAHLYSKGSVVFLGFSGNFAPSWTKLFTSPN
EQGLGVLEQFLPLQMEEYGCSRTGSVHRRDLDLLQQALKVL

TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-NotI Cloning Scheme for this gene | Plasmid Map
ACCN BC019745
ORF Size 2223 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019745, AAH19745
RefSeq Size 2374 bp
RefSeq ORF 2225 bp
Locus ID 83921
Cytogenetics 19 B
MW 82.8 kDa
Summary Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 5 kDa fragments (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity). Is very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate (By similarity).UniProtKB/Swiss-Prot Function
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"BC019745" in other vectors (3)
SKU Description Size Price
MG210404 Tmem2 (tGFP-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225) 10 ug
$879.00
MR210404L3 Lenti ORF clone of Tmem2 (Myc-DDK-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225) 10 ug
$979.00
MR210404L4 Lenti ORF clone of Tmem2 (mGFP-tagged) - Mouse transmembrane protein 2 (cDNA clone MGC:30634 IMAGE:3675225) 10 ug
$979.00

Plasmid Map for MR210404

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210404
Circular map for MR210404

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