Prox1 (NM_008937) Mouse Tagged ORF Clone
$589.00
$676.00
5 Days*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Prox1 |
| Synonyms | A230003G05Rik; PROX-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008937 |
| ORF Size | 2211 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008937.3 |
| RefSeq Size | 4148 bp |
| RefSeq ORF | 2214 bp |
| Locus ID | 19130 |
| UniProt ID | P48437 |
| Cytogenetics | 1 95.85 cM |
| MW | 83.1 kDa |
| Summary | Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008937" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC205549 | Prox1 (untagged) - Mouse prospero-related homeobox 1 (Prox1), (10ug) | 10 ug |
$676.00
|
|
| MG210370 | Prox1 (tGFP-tagged) - Mouse prospero-related homeobox 1 (Prox1) | 10 ug |
$789.00
$876.00
|
|
| MR210370L1 | Lenti ORF clone of Prox1 (Myc-DDK-tagged) - Mouse prospero-related homeobox 1 (Prox1) | 10 ug |
$976.00
|
|
| MR210370L2 | Lenti ORF clone of Prox1 (mGFP-tagged) - Mouse prospero-related homeobox 1 (Prox1) | 10 ug |
$976.00
|
|
| MR210370L3 | Lenti ORF clone of Prox1 (Myc-DDK-tagged) - Mouse prospero-related homeobox 1 (Prox1) | 10 ug |
$976.00
|
|
| MR210370L4 | Lenti ORF clone of Prox1 (mGFP-tagged) - Mouse prospero-related homeobox 1 (Prox1) | 10 ug |
$976.00
|
|
Citations
RNA splicing is a key mediator of tumour cell plasticity and a therapeutic vulnerability in colorectal cancer
Hall, Pohl, Cammareri et al
Nat Commun (2022) 13 (1), 2791 DOI: 10.1038/s41467-022-30489-z
Hall, Pohl, Cammareri et al
Nat Commun (2022) 13 (1), 2791 DOI: 10.1038/s41467-022-30489-z
Cited by 37
AI Summary: OriGene's Prox1 cDNA (MR210370) was used in this study to investigate the role of specific genes in RNA splicing and tumour cell plasticity in colorectal cancer.
Snippet: lli/mouse and 82 corresponding crypts/mouse, using 3–5 mice per genotype. cDNA constructs and SRSF1 mutagenesis Srsf1 cDNA was purchased from GeneCopoeia (EX-Mm13449-Lv122) and Prox1 cDNA was from Origene (MR210370). Kras 4 A and Kras 4B were cloned from cDNA from Apcfl/fl mice. The Srsf1 RNA recognition motif 2 (RRM2) mutant ‘DK’ was created by forcing amino acids 136D and 138 K both
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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