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Prox1 (NM_008937) Mouse Untagged Clone

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SKU
MC205549
Prox1 (untagged) - Mouse prospero-related homeobox 1 (Prox1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $676.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Prox1
Synonyms A230003G05Rik; PROX-1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC051411
GCTGAGAGAGCGCTGGGAAAGCGACGGCCACTGCAGCCGGGGAAGCCGCGCGGCGCACACAAGCCTCTCC GCCGTGCCCGCTGCTCCTCGGGCCACCGCTCCGCGCTCCAGCCCTCGCCGCCTTCTCCGCTCTCTCTCCC CTTCTTTCCCCGGGCTCCCGGCTCTGCCCTGCGCGCCTCTCCCCTCCCCTCCCTCCTTTCTCCCCTCGCC TCTCAGCACTCGCCCAGATCACTCGCTCCCGCACAGACTCACCGACCCCTGGGCCAATTATCACCAGTAA GACATCACCGCGTGCGCGCGTGGGTTTCTTCTCTGCCCGCAAGAGAAGAAGAGAGGGGGAGAGAGAGAGA GAGTGAAAAGAAAGAGAGAGAGAAAGAGAGAGAGTGGCTGCGCCCCGCTGCTGCCCGCACCGCGGTCCCG GGATTCTTGAGCTGTGCCCAGCTGACGAGCTTTTGAAGATGGCACAATAACTGTCCAGTGATGCCTGACC ATGACAGCACAGCCCTCTTAAGCCGGCAAACCAAGAGGAGAAGGGTTGACATTGGAGTGAAAAGGACGGT AGGGACAGCATCTGCATTTTTTGCTAAGGCAAGGGCAACATTTTTCAGTGCCATGAATCCCCAAGGTTCA GAGCAGGATGTTGAATATTCTGTGGTGCAACACGCAGATGGGGAAAAGTCGAACGTACTCCGCAAGCTGC TGAAGAGGGCGAACTCGTATGAAGATGCCATGATGCCTTTTCCAGGAGCAACTATAATTTCCCAGCTGTT GAAAAATAACATGAACAAAAACGGTGGCACCGAGCCCAGTTTCCAAGCCAGCGGACTCTCTAGCACAGGC TCCGAAGTACATCAGGAGGATATATGTAGCAACTCTTCAAGAGACAGCCCCCCAGAGTGTCTTTCCCCTT TTGGCAGGCCTACTATGAGCCAGTTTGATGTGGATCGCTTATGTGATGAGCACCTGAGAGCAAAGCGCGC CCGGGTTGAGAATATCATTCGGGGTATGAGCCATTCCCCCAGTGTGGCATTAAGGGGCAATGAAAACGAA AGAGAGATGGCCCCGCAGTCTGTGAGTCCCCGAGAAAGTTACAGAGAAAACAAACGCAAGCAGAAGCTGC CCCAGCAGCAGCAACAGAGTTTCCAGCAGCTGGTTTCAGCCCGAAAAGAACAGAAGCGAGAGGAGCGCCG ACAGCTGAAACAGCAGCTGGAAGACATGCAGAAGCAGCTGCGCCAGCTGCAGGAGAAGTTCTACCAGGTC TATGACAGCACAGACTCCGAAAATGATGAAGATGGCGACCTGTCTGAAGACAGCATGCGCTCGGAGATCC TGGATGCACGGGCCCAGGACTCGGTGGGGCGCTCAGACAATGAGATGTGTGAGCTGGACCCAGGGCAGTT CATCGACAGGGCCCGAGCCCTAATCAGGGAGCAGGAGATGGCTGAGAACAAGCCTAAGCGAGAAGGCAGC AACAAAGAAAGAGACCACGGGCCAAACTCCTTGCAGCCAGAAGGCAAGCATCTGGCAGAGACCTTAAAAC AGGAGCTGAACACGGCCATGTCGCAGGTTGTGGACACGGTGGTCAAAGTCTTCTCAGCCAAACCCTCTCG CCAGGTTCCTCAGGTCTTCCCACCTCTCCAGATCCCCCAGGCCAGATTCGCAGTCAACGGGGAAAACCAC AATTTCCACACGGCCAACCAGCGCCTGCAATGCTTTGGTGATGTCATCATTCCGAACCCCTTGGACACCT TTGGCAGTGTGCAGATGCCTAGTTCCACAGACCAGACGGAAGCCCTTCCCCTGGTGGTCCGAAAAAACTC ATCCGAGCAATCTGCCTCTGGCCCGGCCACTGGCGGCCACCACCAGCCCCTGCACCAGTCACCCCTCTCC GCCACTGCAGGCTTCACCACCCCTAGCTTCCGCCATCCCTTTCCCCTGCCCTTGATGGCTTATCCATTTC AGAGTCCACTAGGTGCTCCCTCCGGCTCCTTCTCGGGGAAGGACAGAGCCTCTCCTGAGTCCTTAGACTT GACTCGGGACACAACAAGTCTGAGGACCAAGATGTCATCACACCATCTGAGCCACCACCCCTGTTCACCA GCACACCCACCCAGCACCGCAGAAGGACTCTCTTTGTCACTCATAAAGTCTGAGTGTGGAGATCTTCAAG ATATGTCCGACATCTCACCTTATTCAGGAAGCGCAATGCAGGAAGGGCTATCACCCAATCACTTGAAAAA GGCAAAACTCATGTTCTTTTACACCCGCTACCCCAGCTCCAACATGCTGAAGACCTACTTCTCGGACGTG AAGTTCAACAGATGCATTACCTCGCAGCTCATCAAGTGGTTCAGCAATTTCCGTGAGTTTTACTATATCC AGATGGAGAAGTATGCGCGTCAAGCCATCAATGATGGAGTCACCAGTACAGAAGAGCTCTCCATCACCAG GGATTGTGAGCTATACCGAGCCCTCAACATGCACTACAACAAAGCAAATGACTTTGAGGTTCCAGAGAGA TTCCTGGAAGTTGCGCAGATCACGTTACGGGAGTTTTTCAATGCCATCATCGCGGGCAAAGATGTTGATC CTTCCTGGAAGAAGGCCATTTACAAGGTCATCTGCAAGCTGGATAGTGAAGTTCCTGAGATTTTCAAATC CCCTAACTGCCTACAAGAACTCCTTCACGAGTAGAGATTGCAACGCTCTTTTGAATGTATGGATAGAAGA ATTCCCCCTTGGATGTCCAACTTCTCTGTGTCTAGATTTTGGTTTTATATATTTGTGTGGGGGAGGCATG GATATGTTGTGATGTCAGCTGTTGGTTCCTGCTCATCTCTGGGTTCCTTTTCTTTTCCTTCATCACAAGG GGATGGGATGGGCGTTTTCCTTTAGTTTACTTTTGCCCAAGGCCCTTAATATTTGGAGACTTAAGGTAGG GTTGGTTTTCAGGAGAAGGGAATGTCAGCCTGTGTAAAGTGCACGTGAGCTATGGAGTGAGCCAGTGACT GTGACACAGCTCACATGCATACCCCATGCTGTGTCTCCTGTTTCTCTAGGGTTAAGAAGTTCTGGAAGAA CCCGAGGAAAACACTTCACACACTGGAATACCTTGTTTGATTTGAGCCTGATAAGCAAGCTTTTCTTAAA CATCCAAACCAATGCCCTCGGCAGCTGGGTAGCTCACATCTCAAAAGTATCCTCAAAAGACAGAGTTTTA GAAAGATTGTCACCAAGTCCTTTGTAAGAGAAATGGCATAAACCCCCAGACCCAAGTTTGTACAAAACCG ACTCTCCTGAGACAAGGCTGGGAACGCACGCAGGCCAACCTGCTGTCTCTGTGAACTTTAGAACAGGCTC GTGGTGAGATGTGAGCTTGGCCGTGTGGACTTAGAGGAAACCCTGTGCTTTGTCACTAGTTGCTATTTAC TTTGCTTCAGATATTAAAAGGCATGAACTTTCCAAATGTATCCTTTGCTTGACTCTCTTTATACGCTTAG CATTGCTGTTGCTGTTTAACCTTCTTTCTTCTCCTAAAACACTAATTTCTAGATACCTTTCTACAGTAAG GAATTAAGAAGTCCCACTGACTACGAAACCCTTGCCCACTCTTGGAGCACGCTGGCAGCTGCTAGTGGAC CTGATCTTTACTTCCTGTGAGGACATCACCCAGGAATGGCTCATTTGCATATGGGTATGGAGGGTGGCTG TCTGTCTTTTCCCTCTGGGTTGTAATATCTTAAAGCAGCTGACCAGGTCTCTTCTACTACAGAAGCTAAT ATTGACCCAAGTGTGTACAATAGGTTAGTTTGGGGTTACCCAAAAAGACACAACTCCAGCATACTGAGAA AGCCGACTTCTCTCTCTCCCACTCTCAGATGGCTCTTTCCAAACACCCAGGTTTTTGATAATGTACCACC TCCTACACGTGCATGCTAACTATCGCTCACGTTTGTTTTCTTAGCTGGATTTTTGTGTACGGGTGCAGTA AACAGCACTGTTCACTCTAAGAGTGTGATCCAGGCCCCTACTTCCCCTTAGACAATCTGATGTGCACACT GTAAAAATGACACAGCAACAGATCCAGTATTTAAAACATAAAGGACAGAAATGCCATTTCCCCTTAATTT CTTAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_008937
Insert Size 2214 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC051411, AAH51411
RefSeq Size 4148 bp
RefSeq ORF 2214 bp
Locus ID 19130
UniProt ID P48437
Cytogenetics 1 95.85 cM
Summary Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_008937" in other vectors (6)
SKU Description Size Price
MG210370 Prox1 (tGFP-tagged) - Mouse prospero-related homeobox 1 (Prox1) 10 ug
$789.00 $876.00
MR210370 Prox1 (Myc-DDK-tagged) - Mouse prospero-related homeobox 1 (Prox1) 10 ug
$589.00 $676.00
MR210370L1 Lenti ORF clone of Prox1 (Myc-DDK-tagged) - Mouse prospero-related homeobox 1 (Prox1) 10 ug
$976.00
MR210370L2 Lenti ORF clone of Prox1 (mGFP-tagged) - Mouse prospero-related homeobox 1 (Prox1) 10 ug
$976.00
MR210370L3 Lenti ORF clone of Prox1 (Myc-DDK-tagged) - Mouse prospero-related homeobox 1 (Prox1) 10 ug
$976.00
MR210370L4 Lenti ORF clone of Prox1 (mGFP-tagged) - Mouse prospero-related homeobox 1 (Prox1) 10 ug
$976.00

file-alt Citations

Prospero-related homeobox 1 (Prox1) functions as a novel modulator of retinoic acid-related orphan receptors α- and γ-mediated transactivation
Takeda, Jetten
Nucleic Acids Res. (2013) 41 (14), 6992-7008 DOI: 10.1093/nar/gkt447
Cited by 31
AI Summary: OriGene contributed to this study by providing the mouse Prox1 cDNA, which was used to investigate Prox1's role as a co-repressor of RORα and RORγ transcriptional activity.
Snippet: by the Institutional Animal Care and Use Committee at the NIEHS. Plasmids. Mouse Prox1 cDNA was purchased from OriGene (Rockville, MD, USA). The pCMV10-3× Flag-RORγ, pCMV10-3xFlag-RORα, the corresponding ΔAF2
FigureFigureFigure
Showing 1-1 of 1 papers.
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