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Scyl3 (NM_028776) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210356
Scyl3 (Myc-DDK-tagged) - Mouse SCY1-like 3 (S. cerevisiae) (Scyl3)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $674.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Scyl3
Synonyms 1200016D23Rik; 6030457O16; AW214499; Pace1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210356 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGATCAGAGAACAGCGCTTTAAAGAGCTACACACTGAGGGAGTCTCCTTTTACCTTACCATCTGGAC
TTGCTGTTTATCCCGCCATATTGCAAGATGGGAAATGTGCTTCCGTGTTTGTATATAAGCGAGAAAATGA
AGACAAGGTTAACAAGGCTGCCAAGCACTTAAAGACACTCCGTCACCCTTGCTTGCTAAGATTTTTATCT
TGTACTGTGGAAGCAGATGGCATTCATCTCGTCACTGAGAGAGTGCAGCCTCTGGAAGTGGCCTTGGAAA
CCCTGTCTCCTGCAGAAGTCTGTGCTGGAATCTATGACATACTGCTGGCTCTTATCTTCCTTCATGACAG
AGGACATTTGACACACAACAATGTCTGCCTGTCGTCTGTTTTTGTGAGTGAGGATGGGCACTGGAAGCTT
GGAGGCATGGAGACTGTTTGCCAAGTTCCTCAGGCCACGCCAGAGTTTCTCAGGAATATCCAGTCAGTAA
GAGACCCAGCATCCATTCCTCCTGAGGAGATGTCTCCAGAATTTTCAGGCCTTCCCGAGTCCCACGGCCA
CGCCCGGGATGCCTATGCCTTTGGAGCTTTGGTGGACAGCTTGCTCCCCATCTTCAATGAGCAGGTTTCA
GCGGACGTTCTCTCCAGCTTCCTACAGATCCTGCACTCAGCTCTGCTGAACCCCATGCCCGAGTGTCGGC
CAGCACTCAGCACCTTACTGTCCCATGATTTCTTCAGAAATGATTTTCTAGAAGTTGTGAACTTCTTGAA
AAGCTTGACATTAAAGAGTGAAGATGAAAAGACTGAATTTTTCAAATTTCTGCTGGACCGAGTGAGCTGC
CTGTCAGAAGAATTAATAGCTTCAAGATTGGTGCCTCTTCTGCTTAACCAGCTGGTGTTTGCTGAGCCAG
TAGCTGTTAAGAGCTTTCTTCCCTATCTACTTGGTCCTAAAAAAGAGAACGCACCAGGAGAAACCCCTTG
CTTGCTCTCGCCAGCGCTGTTCCAGTCCCGAGTGATCCCTGTGCTTCTCCGGCTGTTCGAGGTGCACGAG
GAGCACGTGCGGATGGTGTTACTGTCCCACATCGAGGCCTACGTGGAGCACTTCACTCAGGAACAGCTAA
AGAAAGTCATCCTGCCACAGGTACTACTGGGCCTTCGTGATACCAGCAATTCCATTGTGGCAATTACTCT
CCGTAGCCTGGCAGTGCTGGTGTCTTTACTTGGACCAGAAGTGGTTGTGGGAGGAGAACGAACCAAGATC
TTTAAGCGCACCGCTCCAAGCTTCACCAAAACTAGTGACCTTTCCCCTGAAGGTTCTCCCATGCATGTTG
TCTGCAGCCAGCAGAGTCGGGTCTCAAAAGTCTTGGAGGACCCCTCGTCTAACGTATTCCCTAAATGGTT
GTCTGGCAACGTGCCCAGCAGCAGCAGGAAGCGCATACAGGAAGAATGTTACAGCTCTCTCTCACAGACA
GGTGATCAGTTTTCTCACACCATTAAATTCCCTATGAATGGACTCTCAGATGTGAAAAACACTTCAGAGG
ACAATGGAAGCTTCCCTGCAGGTTCTAATAAGCCCGAGGAGTGGCCTGACTGGAGCGAGCCTGAGGAGCC
TGAGCAGCAGCCTGCCAGTATTCACAGATGGCCCCGAGAACCCTGTGATGTAGCAGAGTCCCAGCACACA
AACCTGACCGCAGAGGAGGTGACCTGGGATGATGGTGAGGCTAGCTTTGGCACTGAAATAAACTCAACAG
CTACAGCCTCTGCTCCTGTGCCTGTTACCTCAGGGGGACAGTCAACCTCTGCAGCTTTGGTTCCTCTCAC
TGAAGAGTCAAAGCCTTTGCAATCCAGTCCTTCCTCAAAGACCAGTCATAGACAGCATGAGGAAGTCAAA
CCACCCCAAGTGTCACAAGAAAGACCCCTGAAGGCTCCGTCAGGACTTGGTTTGGGAGAAGAGTTTACCA
TTCAAGTAAAAAAGAAGCCAGTACAAGACCCGGAGTTGGACTGGTTTGCAGATATGATCCCAGAAATTAA
GCCTTCAGGTACTTTTCTTATTCTGCCTGAACTAAGGACAGAAGTGATGGTCCCTGACAAGGATAACGTC
TCATCACTGATGCAGTTTTCCTCAAAGTTTGCTGCAACAGAAATGACCGAGGGAGAGGCAGAAGGCTGGG
AAGGAGAAGAGCTAGCCTGGGAAGATAACAACTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210356 protein sequence
Red=Cloning site Green=Tags(s)

MGSENSALKSYTLRESPFTLPSGLAVYPAILQDGKCASVFVYKRENEDKVNKAAKHLKTLRHPCLLRFLS
CTVEADGIHLVTERVQPLEVALETLSPAEVCAGIYDILLALIFLHDRGHLTHNNVCLSSVFVSEDGHWKL
GGMETVCQVPQATPEFLRNIQSVRDPASIPPEEMSPEFSGLPESHGHARDAYAFGALVDSLLPIFNEQVS
ADVLSSFLQILHSALLNPMPECRPALSTLLSHDFFRNDFLEVVNFLKSLTLKSEDEKTEFFKFLLDRVSC
LSEELIASRLVPLLLNQLVFAEPVAVKSFLPYLLGPKKENAPGETPCLLSPALFQSRVIPVLLRLFEVHE
EHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGLRDTSNSIVAITLRSLAVLVSLLGPEVVVGGERTKI
FKRTAPSFTKTSDLSPEGSPMHVVCSQQSRVSKVLEDPSSNVFPKWLSGNVPSSSRKRIQEECYSSLSQT
GDQFSHTIKFPMNGLSDVKNTSEDNGSFPAGSNKPEEWPDWSEPEEPEQQPASIHRWPREPCDVAESQHT
NLTAEEVTWDDGEASFGTEINSTATASAPVPVTSGGQSTSAALVPLTEESKPLQSSPSSKTSHRQHEEVK
PPQVSQERPLKAPSGLGLGEEFTIQVKKKPVQDPELDWFADMIPEIKPSGTFLILPELRTEVMVPDKDNV
SSLMQFSSKFAATEMTEGEAEGWEGEELAWEDNNW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028776
ORF Size 2205 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028776.3
RefSeq Size 4386 bp
RefSeq ORF 2208 bp
Locus ID 240880
UniProt ID Q9DBQ7
Cytogenetics 1 H2.1- H2.2
MW 81.3 kDa
Summary May play a role in regulating cell adhesion/migration complexes in migrating cells.UniProtKB/Swiss-Prot Function
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"NM_028776" in other vectors (3)
SKU Description Size Price
MC221146 Scyl3 (untagged) - Mouse SCY1-like 3 (S. cerevisiae) (Scyl3), (10ug) 10 ug
$741.00
MR210356L3 Lenti ORF clone of Scyl3 (Myc-DDK-tagged) - Mouse SCY1-like 3 (S. cerevisiae) (Scyl3) 10 ug
$974.00
MR210356L4 Lenti ORF clone of Scyl3 (mGFP-tagged) - Mouse SCY1-like 3 (S. cerevisiae) (Scyl3) 10 ug
$974.00

Plasmid Map for MR210356

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210356
Circular map for MR210356

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