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Pcca (NM_144844) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210302
Pcca (Myc-DDK-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $663.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pcca
Synonyms C79630
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210302 representing NM_144844
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGCAGTGGGTCAGGACCGTGGCGCTGTTGGCGGCCAGGCGGCATTGGCGGCGGTCCTCGCAGC
AGCAATTGCTGGGGACGCTGAAGCATGCTCCAGTCTATTCATACCAATGCCTAGTGGTGTCCAGAAGTCT
CAGTTCTGTGGAATATGAGCCTAAAGAAAAGACTTTTGATAAAATTCTCATTGCTAACAGAGGAGAAATT
GCCTGTAGGGTTATTAAAACTTGCAAGAAGATGGGCATCAAGACAGTTGCCATTCACAGTGATGTTGATG
CCAGTTCTGTTCACGTGAAAATGGCGGATGAGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTA
CCTCAACATGGATGCCATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACACCCAGGGTATGGA
TTCCTGTCAGAAAACAAAGAGTTTGCAAAGCGTCTGGCAGCAGAAGATGTCACTTTCATTGGACCTGATA
CTCATGCTATTCAAGCCATGGGTGACAAGATAGAAAGCAAACTATTAGCCAAGAGAGCAAAGGTCAACAC
AATCCCTGGTTTTGATGGGGTAGTAAAGGATGCAGATGAAGCTGTCAGAATTGCAAGGGAAATTGGCTAC
CCTGTGATGATCAAGGCCTCAGCAGGCGGTGGTGGGAAAGGCATGCGCATCGCCTGGGATGACGAAGAGA
CCAGGGATGGCTTTAGATTTTCATCCCAGGAAGCTGCTTCTAGTTTTGGTGATGATAGACTACTAATAGA
AAAATTTATCGATAACCCTCGTCATATAGAAATCCAGGTTTTAGGGGATAAACATGGCAATGCTCTGTGG
CTCAATGAGAGGGAGTGCTCGATCCAGAGAAGGAACCAGAAGGTGGTAGAGGAGGCGCCAAGCATTTTTC
TGGATCCTGAAACTCGCCAAGCAATGGGAGAGCAGGCCGTGGCTTTGGCTAAAGCCGTGAAGTATTCTTC
TGCTGGAACTGTGGAATTTCTTGTGGACTCCCAGAAGAATTTTTACTTCTTGGAGATGAATACAAGACTA
CAGGTCGAACATCCTGTCACAGAGTGCATTACTGGCCTGGACTTAGTCCAAGAAATGATCCTTGTTGCTA
AGGGTTACCCACTCAGGCACAAGCAAGAGGATATTCCCATCAGTGGCTGGGCAGTTGAATGTCGGGTTTA
TGCTGAGGACCCCTACAAGTCTTTCGGTTTACCGTCTATTGGGAGGCTGTCCCAGTACCAAGAGCCGATA
CATCTACCTGGTGTCCGAGTTGACAGTGGCATCCAACCAGGAAGTGACATCAGCATCTATTATGATCCTA
TGATTTCAAAGCTAGTCACATATGGGTCTGACAGAGCAGAAGCCCTGAAGAGGATGGAAGACGCACTGGA
CAATTATGTGATCCGGGGTGTTACACACAACATCCCATTGCTCCGGGAGGTGATAATCAACACACGTTTT
GTGAAAGGAGACATCAGCACTAAGTTTCTCTCTGATGTGTATCCTGATGGCTTCAAAGGGCACACGTTAA
CACTGAGTGAGAGAAACCAGTTATTGGCCATTGCATCATCTGTATTTGTGGCATCCCAGCTACGAGCTCA
GCGCTTCCAAGAACATTCAAGAGTACCAGTTATTAGGCCTGATGTGGCTAAGTGGGAGCTCTCGGTAAAG
TTACATGATGAAGATCATACTGTCGTGGCATCTAACAATGGGCCGGCATTTACCGTGGAAGTTGATGGCT
CGAAACTAAATGTGACCAGTACGTGGAACCTGGCGTCACCCTTATTGTCTGTCAACGTTGATGGCACGCA
GAGGACTGTGCAGTGTCTTTCTCGGGAAGCAGGTGGAAACATGAGCATCCAGTTTCTTGGCACAGTGTAC
AAAGTGCACATTTTAACCAAGCTTGCTGCAGAGCTGAACAAATTCATGCTTGAAAAAGTGCCCAAGGACA
CCAGCAGCACTCTGTGCTCCCCGATGCCTGGAGTGGTGGTGGCCGTTTCTGTCAAGCCTGGAGACATGGT
AGCAGAAGGTCAGGAAATCTGTGTGATTGAAGCTATGAAAATGCAGAACAGTATGACAGCTGGGAAAATG
GGCAAGGTGAAATTGGTGCACTGCAAAGCTGGAGACACAGTTGGTGAAGGAGACCTGCTTGTGGAGCTGG
AA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210302 representing NM_144844
Red=Cloning site Green=Tags(s)

MAGQWVRTVALLAARRHWRRSSQQQLLGTLKHAPVYSYQCLVVSRSLSSVEYEPKEKTFDKILIANRGEI
ACRVIKTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYG
FLSENKEFAKRLAAEDVTFIGPDTHAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGY
PVMIKASAGGGGKGMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALW
LNERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNFYFLEMNTRL
QVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPI
HLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEALKRMEDALDNYVIRGVTHNIPLLREVIINTRF
VKGDISTKFLSDVYPDGFKGHTLTLSERNQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVK
LHDEDHTVVASNNGPAFTVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSREAGGNMSIQFLGTVY
KVHILTKLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSMTAGKM
GKVKLVHCKAGDTVGEGDLLVELE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144844
ORF Size 2172 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144844.2, NP_659093.2
RefSeq Size 2603 bp
RefSeq ORF 2175 bp
Locus ID 110821
UniProt ID Q91ZA3
Cytogenetics 14 65.99 cM
MW 80.4 kDa
Summary This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).UniProtKB/Swiss-Prot Function
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"NM_144844" in other vectors (4)
SKU Description Size Price
MC200415 Pcca (untagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), (10ug) 10 ug
$664.00
MG210302 Pcca (tGFP-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) 10 ug
$863.00
MR210302L3 Lenti ORF clone of Pcca (Myc-DDK-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) 10 ug
$963.00
MR210302L4 Lenti ORF clone of Pcca (mGFP-tagged) - Mouse propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca) 10 ug
$963.00

Plasmid Map for MR210302

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210302
Circular map for MR210302

file-alt Citations

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