OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Mcm7 (NM_008568) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR210265
Mcm7 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $659.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Mcm7 or NM_008568"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcm7
Synonyms AI747533; mCDC47; Mcmd7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210265 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTTAAGGACTACGCGATCGAAAAAGAAAAAGTTAAGAAGTTCCTCCAAGAGTTTTATTACGAGA
ATGAGCTTGGAAAGAAGCAGTTCAAGTATGGGACCCAGTTGGTTCATCTGGCTCATCGGGAACAAGTGGC
ACTGTATGTGGACCTGGATGACATAGCTGAAGATGACCCTGAGTTGGTCGACTCAATTTGCGAGAATGCC
AAGCGCTACTCAAGACTGTTTGGTGATGTTGTGCAAGAGCTTTTGCCTGAGTACAAGGAGAAGGAGGTGG
TGAATAAGGATGTCTTAGATGTTTACATTGAACACCGGCTGATGATGGAGCAGCGAAGCAGAGACCCTGG
GGCAGTCCGGAACCCCCAAAACCAGTATCCTTCTGAACTCATGCGAAGATTTGAGTTGTATTTCCGAGGC
CCAAGTAGCAGCAAGCCTCGAGTGATCCGGGAAGTACGAGCTGACTCTGTGGGGAAATTGTTAACTGTGC
GTGGCATTGTCACTCGTGTGTCTGAAGTCAAGCCCAGGATGGTGGTGGCCACATACACTTGTGATCAGTG
TGGGGCAGAGACCTACCAGCCAATCCAGTCTCCCACTTTCATGCCTCTGATCATGTGCCCCAGCCAGGAG
TGCCAGACCAATCGCTCAGGGGGGCGGCTGTACCTACAGACTCGTGGCTCCAAATTTGTTAAATTCCAGG
AAATGAAGATTCAAGAGCATAGTGACCAAGTTCCTGTGGGAAACATCCCTCGTAGCATCACAGTGGTTCT
AGAAGGTGAGAACACAAGAATTGCCCAGCCTGGTGACCATGTCAGCGTCACTGGCATCTTCTTGCCAGTC
CTGCGCACAGGGTTCCAACAGATGGCACAGGGCTTACTCTCAGAAACTTACCTGGAAGCCCACTGGATTG
TGAAGATGACAAAGAGTGACGATGATGTCTCTGGGGCCGGAGAGCTGAGCTCAGAGGAGCTGAAGCAGAT
TGCAGAGGAGGATTTCTATGAGAAGCTGGCCGCTTCTATTGCTCCGGAGATCTATGGACATGAAGATGTG
AAGAAGGCGCTGCTGCTGCTTCTGGTGGGTGGTGTGGACCAGTCTCCTCAAGGCATGAAGATCAGAGGAA
ACATCCACATCTGCCTTATGGGAGACCCTGGTGTGGCCAAATCTCAGCTCCTATCTTACATCGACCGTCT
GGCACCGCGAAGTCAGTACACAACAGGCCGTGGCTCCTCTGGTGTAGGGCTCACAGCAGCTGTGTTGAGA
GACTCTGTGAGTGGAGAACTGACTTTAGAGGGCGGTGCCCTGGTGCTGGCTGACCAGGGCGTGTGCTGCA
TTGATGAGTTTGACAAGATGGCCGAGGCTGACCGCACAGCCATCCATGAGGTTATGGAGCAGCAGACCAT
CTCCATCGCCAAGGCGGGGATTCTCACCACGCTCAATGCCCGATGCTCCATCCTGGCTGCGGCCAATCCT
GCCTACGGCCGATATAATCCCCGCCGCAGTCTGGAGCAGAACGTCCAGCTTCCGGCTGCTCTGCTTTCTC
GATTCGACCTCCTCTGGCTGATTCAAGACCGGCCCGACAGAGACAATGACCTCCGGTTGGCCCAGCACAT
CACCTATGTCCACCAGCACAGCCGGCAGCCCCCTGCCCAATTTGAACCTTTGGACATGAAACTTATGAGA
CGGTACATAGCCATGTGCCACGAGAGACAGCCCACAGTGCCTGAATCTCTGGCTGACTACATCACCGCAG
CATATGTGGAGATGAGGCGAGAGGCCCGGGCCAGTAAGGATGCCACCTATACTTCTGCCCGGACCCTGCT
GGCCATTCTCCGACTTTCCACTGCTCTGGCTCGACTTCGGATGGTAGACATTGTGGAGAAGGAAGATGTA
AATGAAGCCATAAGGTTGATGGAGATGTCGAAGGACTCCCTTCTAGGTGAAAAGGGACAGACAGCTAGGA
CCCAGAGGCCAGCAGATGTGATATTTGCCACCATCCGTGAATTGGTGTCACGGGGGCGGAGTGTCCACTT
TTCTGAGGCTGAACAGCGCTGCATATCTCGTGGCTTCACACCAGCCCAGTTCCAGGCAGCTCTGGATGAA
TATGAGGAGCTAAATGTCTGGCAGGTTAATACTTCTCGGACACGGATCACCTTTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210265 protein sequence
Red=Cloning site Green=Tags(s)

MALKDYAIEKEKVKKFLQEFYYENELGKKQFKYGTQLVHLAHREQVALYVDLDDIAEDDPELVDSICENA
KRYSRLFGDVVQELLPEYKEKEVVNKDVLDVYIEHRLMMEQRSRDPGAVRNPQNQYPSELMRRFELYFRG
PSSSKPRVIREVRADSVGKLLTVRGIVTRVSEVKPRMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQE
CQTNRSGGRLYLQTRGSKFVKFQEMKIQEHSDQVPVGNIPRSITVVLEGENTRIAQPGDHVSVTGIFLPV
LRTGFQQMAQGLLSETYLEAHWIVKMTKSDDDVSGAGELSSEELKQIAEEDFYEKLAASIAPEIYGHEDV
KKALLLLLVGGVDQSPQGMKIRGNIHICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANP
AYGRYNPRRSLEQNVQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPAQFEPLDMKLMR
RYIAMCHERQPTVPESLADYITAAYVEMRREARASKDATYTSARTLLAILRLSTALARLRMVDIVEKEDV
NEAIRLMEMSKDSLLGEKGQTARTQRPADVIFATIRELVSRGRSVHFSEAEQRCISRGFTPAQFQAALDE
YEELNVWQVNTSRTRITFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008568
ORF Size 2157 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008568.3
RefSeq Size 2396 bp
RefSeq ORF 2160 bp
Locus ID 17220
UniProt ID Q61881
Cytogenetics 5 G2
MW 81.2 kDa
Summary Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_008568" in other vectors (4)
SKU Description Size Price
MC205195 Mcm7 (untagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7), (10ug) 10 ug
$659.00
MG210265 Mcm7 (tGFP-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7) 10 ug
$859.00
MR210265L3 Lenti ORF clone of Mcm7 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7) 10 ug
$959.00
MR210265L4 Lenti ORF clone of Mcm7 (mGFP-tagged) - Mouse minichromosome maintenance deficient 7 (S. cerevisiae) (Mcm7) 10 ug
$959.00

Plasmid Map for MR210265

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210265
Circular map for MR210265

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products