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Ehhadh (NM_023737) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210264
Ehhadh (Myc-DDK-tagged) - Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $658.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ehhadh
Synonyms 1300002P22Rik; HD; L-PBE; LBFP; LBP; MFP; MFP1; PBFE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210264 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGTATCTGAGGCTGCCCCACTCCCTGGCTATGATCCGCCTCTGCAATCCACCGGTCAATGCCA
TCAGTCCAACTGTAATCACAGAAGTAAGGAATGGACTCCAGAAAGCTAGTTTGGACCATACGGTTAGAGC
CATAGTGATCTGTGGAGCAAATGACAACTTCTGTGCAGGTGCTGATATCCATGGCTTTAAATCTCCCACT
GGCCTTACATTAGGAAGCTTGGTAGATGAAATACAGCGATACCAGAAGCCAGTGGTGGCCGCCATCCAAG
GCGTGGCTCTTGGAGGAGGACTAGAGCTGGCCTTGGGCTGTCACTATCGGATTGCCAATGCAAAGGCTCG
TGTTGGCTTCCCGGAAGTGATGCTGGGAATTCTTCCTGGTGCAAGAGGAACGCAGCTTCTACCCAGGGTC
GTTGGAGTTCCTGTTGCTCTTGACTTAATTACCTCAGGAAGACATATTTCAACAGATGAAGCACTCAAGC
TTGGAATTCTGGATGTAGTTGTAAAGTCAGACCCAGTTGAAGAAGCCATCAAATTTGCTCAGACGGTTAT
AGGTAAACCCATAGAACCCCGCAGGATCCTAAACAAGCCAGTCCCAAGCTTGCCCAACATGGACAGTGTT
TTTGCAGAAGCCATTGCCAAGGTACGGAAGCAGTACCCTGGCCGCCTGGCTCCGGAGACTTGTGTCCGTT
CAGTCCAGGCCTCCGTGAAGCATCCATATGAAGTGGCCATCAAGGAAGAAGCAAAGCTGTTTATGTACCT
TCGGGGGTCGGGGCAGGCTAGAGCCCTGCAGTACGCCTTTTTTGCTGAAAAGTCTGCAAATAAGTGGTCA
ACTCCCTCAGGAGCATCTTGGAAAACAGCATCTGCTCAACCCGTCTCCTCGGTTGGTGTTCTTGGCTTGG
GAACGATGGGCCGAGGCATCGCCATCTCTTTTGCAAGGGTGGGGATCCCTGTGGTTGCTGTAGAGTCAGA
CCCAAAGCAGCTAGATACTGCAAAGAAGATAATAACTTCCACCTTGGAAAAGGAAGCATCGAAGAGTGGC
CAAGCTTCAGCAAAACCAAACCTCAGGTTCTCCTCATCCACAAAGGAGCTTTCGAGTGTGGATTTAGTGA
TTGAAGCAGTGTTCGAAGATATGAACCTGAAGAAGAAGGTCTTCGCTGAACTGTCAGCCCTGTGCAAGCC
GGGAGCCTTTCTGTGCACCAATACCTCAGCACTGGATGTGGATGACATTGCTTCTTCCACAGATCGCCCC
CAGCTGGTGATTGGCACCCACTTCTTCTCCCCAGCCCACATCATGAGGTTACTAGAGGTCATTCCTAGCC
GATACTCTTCCCCCACTACCATCGCCACAGTCATGAGCTTATCCAAAAGGATTGGAAAGATTGGAGTCGT
TGTTGGTAACTGCTATGGATTTGTTGGGAATCGAATGTTGGCTCCCTATTACAACCAGGGCTATTTCTTG
ATAGAGGAAGGTAGTAAGCCAGAGGATGTAGATGGGGTCTTAGAAGAGTTTGGTTTTAGAATGGGACCCT
TCAGGGTGTCTGACCTCGCAGGGCTAGATGTGGGTTGGAAAGTTCGCAAAGGGCAAGGCCTTACTGGACC
GTCCTTACCTCCAGGAACCCCCACCCGAAAGAGGGGCAATACCAGGTACTCCCCAATTGCTGATATGCTC
TGTGAAGCTGGGCGATTTGGTCAGAAGACAGGTAAGGGCTGGTATCAGTATGACAAGCCACTGGGTCGCA
TCCACAAACCTGATCCCTGGCTTTCTGAGTTTCTGTCACAGTATAGAGAAACCCATCACATCAAGCAGCG
CTCCATCAGCAAGGAGGAAATCCTGGAGCGTTGCTTATATTCCCTTATCAACGAGGCATTCCGCATCTTG
GAGGAGGGGATGGCCGCTAGCCCAGAGCACATTGATGTCATCTACTTGCATGGGTATGGGTGGCCAAGGC
ACGTGGGTGGGCCCATGTACTATGCTGCCTCAGTTGGGCTGCCCACAGTTCTAGAGAAATTGCAGAAATA
TTACAGACAGAATCCTGACATCCCCCAGCTGGAGCCCAGTGACTACCTGAGGAGGCTGGTTGCCCAGGGA
AGCCCTCCTCTGAAAGAATGGCAAAGCTTGGCAGGACCCCATAGCAGCAAACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210264 protein sequence
Red=Cloning site Green=Tags(s)

MAEYLRLPHSLAMIRLCNPPVNAISPTVITEVRNGLQKASLDHTVRAIVICGANDNFCAGADIHGFKSPT
GLTLGSLVDEIQRYQKPVVAAIQGVALGGGLELALGCHYRIANAKARVGFPEVMLGILPGARGTQLLPRV
VGVPVALDLITSGRHISTDEALKLGILDVVVKSDPVEEAIKFAQTVIGKPIEPRRILNKPVPSLPNMDSV
FAEAIAKVRKQYPGRLAPETCVRSVQASVKHPYEVAIKEEAKLFMYLRGSGQARALQYAFFAEKSANKWS
TPSGASWKTASAQPVSSVGVLGLGTMGRGIAISFARVGIPVVAVESDPKQLDTAKKIITSTLEKEASKSG
QASAKPNLRFSSSTKELSSVDLVIEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALDVDDIASSTDRP
QLVIGTHFFSPAHIMRLLEVIPSRYSSPTTIATVMSLSKRIGKIGVVVGNCYGFVGNRMLAPYYNQGYFL
IEEGSKPEDVDGVLEEFGFRMGPFRVSDLAGLDVGWKVRKGQGLTGPSLPPGTPTRKRGNTRYSPIADML
CEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSEFLSQYRETHHIKQRSISKEEILERCLYSLINEAFRIL
EEGMAASPEHIDVIYLHGYGWPRHVGGPMYYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRLVAQG
SPPLKEWQSLAGPHSSKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023737
ORF Size 2154 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023737.3
RefSeq Size 3010 bp
RefSeq ORF 2157 bp
Locus ID 74147
UniProt ID Q9DBM2
Cytogenetics 16 B1
MW 78.3 kDa
Summary Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long straight chain fatty acids peroxisomal beta-oxidation pathway. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species. Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (Probable). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (PubMed:24075987). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_023737" in other vectors (4)
SKU Description Size Price
MC201157 Ehhadh (untagged) - Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh), (10ug) 10 ug
$658.00
MG210264 Ehhadh (tGFP-tagged) - Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh) 10 ug
$858.00
MR210264L3 Lenti ORF clone of Ehhadh (Myc-DDK-tagged) - Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh) 10 ug
$958.00
MR210264L4 Lenti ORF clone of Ehhadh (mGFP-tagged) - Mouse enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase (Ehhadh) 10 ug
$958.00

Plasmid Map for MR210264

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210264
Circular map for MR210264

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