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Agbl5 (BC057349) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210236
Agbl5 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 5 (cDNA clone MGC:67138 IMAGE:6813901)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $656.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Agbl5
Synonyms 4930455N08; 9430057O19Rik; CCP5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210236 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACATGAACAAGCAAAGCAAACTGTATTCCCAGGGCATGGCCCCCTTTGTGCGCACACTGCCTTCCC
GGCCACGCTGGGAACGCATTAGAGAGCGGCCCACCTTTGAGATGACAGAGACACAGTTTGTGCTATCCTT
TGTTCACCGTTTCGTAGAGGGCCGGGGAGCCACCACCTTCTTCGCCTTCTGCTACCCCTTCTCCTACAGT
GACTGCCAGGATCTGCTGAGCCAGCTAGACCAGCGCTTTTCAGAGAATTACTCTACCCATAGCAGTCCTC
TGGATAGTATTTATTACCACCGAGAGCTTCTCTGCTATTCTCTGGATGGACTTCGTGTAGATCTACTAAC
AATCACTTCCTGCCATGGGCTTCGAGATGATCGGGAGCCCCGACTAGAGCAGCTATTTCCTGATCTCGGC
ACTCCCCGACCATTCCGTTTCACAGGCAAAAGGATATTCTTCTTAAGCAGTAGGGTACACCCTGGAGAGA
CTCCATCTAGCTTTGTGTTCAATGGCTTTCTGGACTTCATCCTTCGACCTGATGATCCCCGGGCACAAAC
TCTCCGTCGACTCTTTGTTTTTAAGTTGATTCCCATGTTAAACCCTGATGGTGTGGTCCGGGGCCACTAC
CGCACGGACTCCCGTGGAGTGAATCTGAACCGTCAGTACTTGAAGCCTGATGCCGTCCTGCACCCGGCCA
TCTATGGCGCCAAAGCTGTGCTGCTTTATCATCACGTGCACTCGAGGCTCAACGCCAAGAGTCCCACGAA
CCAGCAACCCACTCTCCATCTCCCTCCGGAAGCCCCTCTCTCTGACCTCGAGAAAGCCAATAATCTCCAC
AATGAAGCTCACCTCGGGCAGTCACCTGATGGGGAAAACCCTGCGACTTGGCCTGAGACAGAGCCAGCAG
AAGAGAAGACTGACCCTGTGTGGCTTATGCCACAGCCAATTCCTGAACTCGAGGAGCCAGCCCCTGACAC
CATCCCCCCAAAAGAGAGTGGTGTTGCTTACTATGTAGACTTGCACGGACATGCTTCCAAAAGGGGCTGC
TTCATGTATGGCAACAGCTTTAGTGACGAGAGCACCCAGGTGGAAAACATGCTATATCCAAAGCTCATCT
CCTTGAATTCAGCCCACTTTGACTTCCAGGGCTGCAATTTCTCAGAGAAGAACATGTATGCCCGAGACCG
TAGAGATGGCCAGTCCAAAGAGGGAAGCGGCCGTGTCGCAATCTACAAAGCCTCAGGGATAATCCACAGC
TACACACTTGAATGCAACTACAACACTGGACGCTCAGTCAACAGCATCCCTGCTGCCTGCCATGACAACG
GGCGCGCCAGCCCCCCTCCCCCTCCGGCTTTCCCCTCCAGATACACTGTGGAACTATTCGAGCAGGTGGG
GCGAGCTATGGCCATCGCAGCTCTGGACATGGCAGAGTGTAATCCGTGGCCCCGAATTGTGCTGTCGGAA
CACAGCAGCCTCACCAACCTGCGAGCCTGGATGCTGAGGCACGTGCGCAACAGCAGAGGGCTGACCAGTG
CTGGGAACATGGGCGCGAGCAAGAAGCGAGGGGCTCGAACCCCTCCCAAAAGCAACAACAGCTTGCCTGT
TTCCTGCTCTGAAAATGCTCTGAGCCGGGTTCGCAGTTTTAGCACCGGCACGAGTACTGGTGGCAGCAGC
AGCAGCCAACAAAACTCTCCACAGATGAAGAACTCTCCCAGCTTCCCTTTTCATGGCAGTCGGACTGCGG
GGCTACCAGGCCTGGGCTCTAGCACCCAAAAGGTCAGCCATCGGGTGCTGGGCCCTGTCAGAGAGCCCCG
ATGCTCAGACAGAAGGCGGCGACAGCAGCCACTGAACCATCGCTCTACCACAAGCAGCCTGGCCCCATCC
CCAACTCTGGCTAGTTCTGGCCCAACCTCCTCACGAAACATGGGCTCCTGCCTCCTACCCAATTCACTCA
GCTTATCAGGGAGCAGCTGCTCATTCTCGTCTTCAGGGGACAAGCCAGAGGCTGTCATGGTGATTGGGAA
AAGCCTACTAGGGGCTGGTGCTCGGATCCCTTGCATTAGGACTCGATTGCAGACTCCATTGTCCCTGCCA
GATGCACCCCAACCACAGCCTACTAGCTTTGGCTTTCCTACCCGTCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210236 protein sequence
Red=Cloning site Green=Tags(s)

MNMNKQSKLYSQGMAPFVRTLPSRPRWERIRERPTFEMTETQFVLSFVHRFVEGRGATTFFAFCYPFSYS
DCQDLLSQLDQRFSENYSTHSSPLDSIYYHRELLCYSLDGLRVDLLTITSCHGLRDDREPRLEQLFPDLG
TPRPFRFTGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHY
RTDSRGVNLNRQYLKPDAVLHPAIYGAKAVLLYHHVHSRLNAKSPTNQQPTLHLPPEAPLSDLEKANNLH
NEAHLGQSPDGENPATWPETEPAEEKTDPVWLMPQPIPELEEPAPDTIPPKESGVAYYVDLHGHASKRGC
FMYGNSFSDESTQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHS
YTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMAECNPWPRIVLSE
HSSLTNLRAWMLRHVRNSRGLTSAGNMGASKKRGARTPPKSNNSLPVSCSENALSRVRSFSTGTSTGGSS
SSQQNSPQMKNSPSFPFHGSRTAGLPGLGSSTQKVSHRVLGPVREPRCSDRRRRQQPLNHRSTTSSLAPS
PTLASSGPTSSRNMGSCLLPNSLSLSGSSCSFSSSGDKPEAVMVIGKSLLGAGARIPCIRTRLQTPLSLP
DAPQPQPTSFGFPTRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC057349
ORF Size 2148 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC057349, AAH57349
RefSeq Size 3332 bp
RefSeq ORF 2150 bp
Locus ID 231093
Cytogenetics 5 B1
MW 79.5 kDa
Summary Metallocarboxypeptidase that mediates protein deglutamylation. Specifically catalyzes the deglutamylation of the branching point glutamate side chains generated by post-translational glutamylation in proteins such as tubulins (PubMed:20519502, PubMed:21074048). In contrast, it is not able to act as a long-chain deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/CCP1, AGBL2/CCP2, AGBL3/CCP3, AGBL1/CCP4 and AGBL4/CCP6 (PubMed:25103237). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768).UniProtKB/Swiss-Prot Function
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"BC057349" in other vectors (4)
SKU Description Size Price
MC220676 Agbl5 (untagged) - Mouse ATP/GTP binding protein-like 5 (cDNA clone MGC:67138 IMAGE:6813901), (10ug) 10 ug
$722.00
MG210236 Agbl5 (tGFP-tagged) - Mouse ATP/GTP binding protein-like 5 (cDNA clone MGC:67138 IMAGE:6813901) 10 ug
$856.00
MR210236L3 Lenti ORF clone of Agbl5 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 5 (cDNA clone MGC:67138 IMAGE:6813901) 10 ug
$956.00
MR210236L4 Lenti ORF clone of Agbl5 (mGFP-tagged) - Mouse ATP/GTP binding protein-like 5 (cDNA clone MGC:67138 IMAGE:6813901) 10 ug
$956.00

Plasmid Map for MR210236

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210236
Circular map for MR210236

file-alt Citations

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