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Reps1 (BC087547) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210234
Reps1 (Myc-DDK-tagged) - Mouse RalBP1 associated Eps domain containing protein (cDNA clone MGC:91085 IMAGE:30441027)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $656.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Reps1
Synonyms BB161292
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210234 representing BC087547
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTTTGTGGTGCAACAAGACTTGGTTATTTTGGAAGAAGTCAGTTCTACATTGCTTTGAAGCTTG
TAGCTGTTGCCCAGTCTGGTTTTCCATTAAGAGTGGAAAGTATAAACACTGTAAAGGATCTCCCTCTCCC
ACGATTTGTTGCTTCAAAGAATGAACAGGAATCTCGCCTTGCAGCCTCCTATTCTTCAGATTCTGAAAAC
CAGGGCTCTTACTCGGGTGTGATCCCCCCTCCTCCCGGCAGAGGCCAAGTCAAGAAGGGGCCTGGAAGCC
ATGACGCGGTTCAGCCTCGTCCGTCTGCAGAGCAGCAGGAACCTGTGTCTCCAGTAGTTTCACCCCAGCA
GTCCCCACCAACTTCTCCCCACACATGGAGGAAGCACAGCCGCCATCCCAGTGGGGGGAATAGTGAGAGA
CCTCTTACAGGACCGGGGCCATTCTGGTCTCCTTTTGGTGATGCACAAGCAGGCTCCTCTGCTGGTGATG
CGGTGTGGTCTGGACAGTCCCCGCCTCCACCTCAAGACAACTGGGTCAGTTTTGCAGATACTCCACCAAC
CAGTGCTCTTTTAACCATGCATCCTGCTTCTGTCCAGGACCAGACGACAGTACGGACTGTAGCCTCAGCA
GCAACTGCCAATGAGATCCGTAGGCAATCCAGTAGTTATGAGGATCCCTGGAAGATAACAGATGAGCAGA
GACAGTATTATGTAAATCAGTTTAAGACCATTCAGCCTGACCTAAACGGATTTATCCCAGGATCTGCAGC
TAAAGAGTTTTTTACAAAATCAAAACTTCCTATTCTTGAACTTTCTCATATCTGGGAACTCTCAGACTTT
GATAAAGATGGCGCATTGACACTGGATGAGTTTTGTGCTGCTTTTCATCTGGTAGTTGCTAGAAAAAATG
GCTATGACCTACCAGAGAAACTCCCTGAAAGCCTGATGCCCAAGCTGATAGACTTGGAAGATTCGGCAGA
TGTGGGGGAGCAGCCAGGTGAGGTAGGTTACTCAGGCTCTCCTGCAGAAGCACCTCCAAGCAAGTCACCG
TCGATGCCGTCCCTGAACCAGACATGGCCAGAGCTGAATCAGAGCAGTGAGGATACCGCTATTGTGCATC
CAGTCCCCATCCGCATGACTCCAAGCAAAATCCACATGCAGGAAATGGAGCTGAAGAGAACAAGCAGCGA
TCACACTAATCCCACCAGCCCGTTGCTTGTGAAACCATCTGACCTTTCAGAAGAAAATAAAATCAATTCA
TCAGTAAAATTTCCTTCTGGAAATACCGTAGATGGGTACAGTAGCTCAGACTCTTTCCCCTCTGACCCGG
AGCAGATTGGGAGCAGTGTAACTCGTCAAAGGTCTCATTCAGGAACGTCACCTGATAACACTGCGCCACC
ACCTCCCCCTCCCAGGCCACAGCCTTCTCATTCTAGATCATCATCTTTAGATATGAATCGGACCTTTGCA
GTTACCACAGGACAACAACAGGCTGGAGTTGTTGCCCATCCTCCTGCAGTGCCTCCAAGACCTCAGCCCT
CACAGGCACCCGGTCCTTCTGTCCATCGCCCAGTGGATGCAGATGGTCTAATAACTCACACTAGTACCTC
ACCTCAGCAGATACCAGAACAACCAAATTTTGCAGATTTCAGCCAATTTGAAGTATTTGCTGCATCAAAT
GTCTCCGAAGAACAAGACAGTGAAGCTGAGAAACATCCTGAGGTCTTGCCAGCTGAGAAAGCTTCTGACC
CCTCAAGCTCTCTTCGGGCTGCCCAAGCGGACAGTAAAGCTGAAGAAAAGACAGCTACTAATGTTCCTGC
CAATGTGAGCAAAGGCACAACACCTCTGGCTCCACCACCAAAACCTGTTAGAAGAAGATTAAAGTCAGAA
GATGAGTTACGGCCAGATGTGGATGAGCATACGCAGAAGACAGGCGTCCTAGCTGCTGTTCTAACATCAC
AGCCTTCTATTCCCAGATCTGTGGGGAAAGATAAAAAGGCTATCCAGGCATCCATTAGACGAAATAAGGA
AACCAACACGGTTCTGGCCAGGCTGAACAGTGAATTGCAACAGCAATTAAAGGATGTTCTGGAGGAGAGA
ATCTCCCTGGAAGTTCAACTAGAGCAGCTCCGACCGTTTTCTCACCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210234 representing BC087547
Red=Cloning site Green=Tags(s)

MELCGATRLGYFGRSQFYIALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQESRLAASYSSDSEN
QGSYSGVIPPPPGRGQVKKGPGSHDAVQPRPSAEQQEPVSPVVSPQQSPPTSPHTWRKHSRHPSGGNSER
PLTGPGPFWSPFGDAQAGSSAGDAVWSGQSPPPPQDNWVSFADTPPTSALLTMHPASVQDQTTVRTVASA
ATANEIRRQSSSYEDPWKITDEQRQYYVNQFKTIQPDLNGFIPGSAAKEFFTKSKLPILELSHIWELSDF
DKDGALTLDEFCAAFHLVVARKNGYDLPEKLPESLMPKLIDLEDSADVGEQPGEVGYSGSPAEAPPSKSP
SMPSLNQTWPELNQSSEDTAIVHPVPIRMTPSKIHMQEMELKRTSSDHTNPTSPLLVKPSDLSEENKINS
SVKFPSGNTVDGYSSSDSFPSDPEQIGSSVTRQRSHSGTSPDNTAPPPPPPRPQPSHSRSSSLDMNRTFA
VTTGQQQAGVVAHPPAVPPRPQPSQAPGPSVHRPVDADGLITHTSTSPQQIPEQPNFADFSQFEVFAASN
VSEEQDSEAEKHPEVLPAEKASDPSSSLRAAQADSKAEEKTATNVPANVSKGTTPLAPPPKPVRRRLKSE
DELRPDVDEHTQKTGVLAAVLTSQPSIPRSVGKDKKAIQASIRRNKETNTVLARLNSELQQQLKDVLEER
ISLEVQLEQLRPFSHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC087547
ORF Size 2148 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC087547.1
RefSeq Size 2505 bp
RefSeq ORF 2150 bp
Locus ID 19707
Cytogenetics 10 A3
MW 91.9 kDa
Summary May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.UniProtKB/Swiss-Prot Function
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"BC087547" in other vectors (4)
SKU Description Size Price
MC220752 Reps1 (untagged) - Mouse RalBP1 associated Eps domain containing protein (cDNA clone MGC:91085 IMAGE:30441027), (10ug) 10 ug
$722.00
MG210234 Reps1 (tGFP-tagged) - Mouse RalBP1 associated Eps domain containing protein (cDNA clone MGC:91085 IMAGE:30441027) 10 ug
$856.00
MR210234L3 Lenti ORF clone of Reps1 (Myc-DDK-tagged) - Mouse RalBP1 associated Eps domain containing protein (cDNA clone MGC:91085 IMAGE:30441027) 10 ug
$956.00
MR210234L4 Lenti ORF clone of Reps1 (mGFP-tagged) - Mouse RalBP1 associated Eps domain containing protein (cDNA clone MGC:91085 IMAGE:30441027) 10 ug
$956.00

Plasmid Map for MR210234

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210234
Circular map for MR210234

file-alt Citations

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