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Acss2 (NM_019811) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210131
Acss2 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase short-chain family member 2 (Acss2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $642.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Acss2
Synonyms 1110017C11Rik; ACAS; Acas1; Acas2; AceCS1; Acs1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210131 representing NM_019811
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCTTCCCGAGGAGCGGCGCAAGAGCGGCAGTGGAAGCCGGGCTCGTGAGGAGACCGGCGCCGAGG
GCCGGGTGCGGGGTTGGTCCCCGCCGCCGGAGGTCAGACGCTCGGCGCACGTCCCCTCTCTGCAGCGCTA
CCGCGAGCTGCACCGGCGTTCTGTGGAGGAGCCACGGGAGTTTTGGGGAAACATTGCCAAGGAATTTTAC
TGGAAAACCGCATGCCCTGGCCCATTCCTCCAGTACAACTTTGATGTGACTAAAGGGAAAATATTCACTG
AGTGGATGAAAGGAGCAACTACAAACATCTGCTACAACGTGCTGGATCGAAATGTCCATGAGAAAAAACT
TGGCGACAAAGTTGCTTTTTACTGGGAGGGCAATGAGCCAGGGGAGACCACCAAGATCACATACCGTGAA
CTCCTGGTCCAGGTGTGTCAGTTCAGCAATGTTCTCCGTAAACAGGGCATTCAGAAGGGTGACCGAGTGG
CCATCTACATGCCTATGATCTTGGAACTTGTGGTGGCTATGCTGGCATGCGCTCGCCTTGGAGCTTTGCA
CTCCATTGTGTTTGCAGGCTTCTCTGCAGAGTCTCTCTGTGAAAGGATCTTGGATTCCAGTTGCTGCCTG
CTCATCACTACAGATGCCTTCTACAGGGGGGAAAAACTTGTGAACCTGAAGGAGCTGGCTGATGAGTCCT
TGGAGAAGTGCCGAGAGAAGGGCTTCCCAGTGAGATGCTGCATTGTGGTCAAACATCTGGGGCGGGCAGA
GCTGGGCATGAATGACTCCCCCAGCCAGTCCCCACCAGTTAAGAGGCCATGTCCAGATGTCCAGATCTGC
TGGAACGAAGGGGTTGACTTATGGTGGCATGAACTCATGCAGCAGGCAGGAGACGAGTGTGAGCCTGAGT
GGTGTGATGCTGAGGACCCACTCTTCATCTTGTACACCAGTGGCTCCACAGGCAAACCTAAGGGTGTGGT
GCACACAATTGGAGGCTATATGCTCTATGTGGCTACAACTTTCAAGTATGTGTTTGATTTCCACCCGGAA
GATGTGTTCTGGTGCACAGCAGACATCGGCTGGATCACTGGTCATTCCTATGTCACCTATGGGCCACTGG
CTAATGGTGCCACTAGTGTTTTGTTTGAGGGGATCCCCACATACCCAGATGAAGGGCGCTTGTGGAGCAT
TGTGGACAAATACAAGGTGACCAAGTTCTACACGGCACCAACAGCCATCCGGATGCTCATGAAGTTTGGA
GATGATCCTGTCACCAAGCATAGCCGGGCATCCCTGCAGGTGCTGGGCACAGTAGGTGAACCCATCAACC
CTGAAGCCTGGCTATGGTACCACCGGGTAGTAGGTTCCCAGCGCTGCCCCATTGTAGACACCTTCTGGCA
AACAGAAACAGGTGGCCATATGCTGACCCCTCTCCCTGGCGCCACACCCATGAAACCTGGTTCTGCTTCT
TTCCCATTCTTCGGTGTAGCGCCTGCAATCCTGAATGAGTCCGGGGAGGAGCTGGAAGGGGAAGCTGAAG
GTTATCTGGTGTTCAAGCAGCCCTGGCCAGGGATCATGCGCACAGTCTATGGGAACCACACACGGTTTGA
GACCACCTACTTTAAGAAGTTCCCTGGCTACTATGTGACCGGAGATGGCTGCCGACGGGATCAGGATGGC
TATTACTGGATCACGGGCAGGATTGATGACATGCTCAATGTGTCTGGACATCTCCTGAGTACAGCAGAGG
TGGAATCGGCACTTGTGGAACACGAGGCTGTCGCAGAGGCAGCTGTGGTGGGCCACCCTCATCCTGTGAA
GGGCGAATGCCTCTACTGCTTTGTTACCTTGTGTGATGGCCATACCTTCAGCCCCACACTCACAGAGGAA
CTCAAGAAGCAGATTAGAGAAAAGATTGGCCCCATTGCCACACCAGACTACATCCAGAATGCACCTGGCT
TGCCTAAAACACGCTCAGGGAAAATCATGAGGCGAGTTCTCCGGAAGATTGCTCAGAATGACCATGACCT
GGGGGATACATCTACGGTGGCTGACCCATCTGTCATCAACCATCTCTTCAGTCACCGCTGCCTGACCACC
CAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210131 representing NM_019811
Red=Cloning site Green=Tags(s)

MGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFY
WKTACPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRE
LLVQVCQFSNVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCL
LITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQIC
WNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTIGGYMLYVATTFKYVFDFHPE
DVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFG
DDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSAS
FPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDG
YYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEE
LKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTT
Q

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019811
ORF Size 2103 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019811.3, NP_062785.2
RefSeq Size 2833 bp
RefSeq ORF 2106 bp
Locus ID 60525
UniProt ID Q9QXG4
Cytogenetics 2 H1
MW 79.3 kDa
Summary Activates acetate so that it can be used for lipid synthesis or for energy generation.UniProtKB/Swiss-Prot Function
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"NM_019811" in other vectors (4)
SKU Description Size Price
MC202272 Acss2 (untagged) - Mouse acyl-CoA synthetase short-chain family member 2 (Acss2), (10ug) 10 ug
$643.00
MG210131 Acss2 (tGFP-tagged) - Mouse acyl-CoA synthetase short-chain family member 2 (Acss2) 10 ug
$842.00
MR210131L3 Lenti ORF clone of Acss2 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase short-chain family member 2 (Acss2) 10 ug
$942.00
MR210131L4 Lenti ORF clone of Acss2 (mGFP-tagged) - Mouse acyl-CoA synthetase short-chain family member 2 (Acss2) 10 ug
$942.00

Plasmid Map for MR210131

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210131
Circular map for MR210131

file-alt Citations

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