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Capn2 (NM_009794) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210127
Capn2 (Myc-DDK-tagged) - Mouse calpain 2 (Capn2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $641.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Capn2
Synonyms AI326419; Capa-2; Capa2; m-calpain; m-calpin
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210127 representing NM_009794
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCATCGCGATAAAGCTGGCCAAGGACCGCGAAGCGGCCGAGGGGCTGGGGTCGCATGAGAGAG
CCATCAAGTACCTCAACCAGGACTACGAGACGCTGCGGAACGAGTGCCTGGAGGCCGGGGCGCTCTTCCA
GGATCCTTCCTTCCCCGCCCTGCCGTCATCCTTGGGCTATAAGGAGTTGGGGCCCTACTCCAGCAAAACT
CGGGGCATCGAATGGAAGCGGCCCACGGAGATCTGCGCTGACCCCCAGTTCATTATTGGAGGAGCCACCC
GCACAGACATCTGCCAAGGAGCCCTGGGGGACTGCTGGCTGCTGGCTGCCATCGCCTCCCTCACCTTGAA
TGAGGAAATCCTGGCTCGGGTTGTGCCTCCAGATCAGAGCTTCCAGGAAAACTATGCAGGCATCTTCCAC
TTCCAGTTCTGGCAATATGGCGAGTGGGTGGAGGTGGTGGTGGACGACAGGCTGCCCACTAAGGACGGGG
AGCTGCTCTTTGTGCATTCAGCTGAAGGGAGTGAGTTCTGGAGTGCCCTTCTGGAGAAAGCCTATGCCAA
GATCAATGGGTGCTATGAAGCACTCTCAGGGGGTGCCACCACGGAAGGCTTTGAAGACTTCACAGGAGGC
ATTGCTGAGTGGTATGAGCTGAGGAAGCCTCCTCCCAACTTGTTCAAGATCATCCAGAAGGCTTTGGAGA
AAGGTTCTCTGCTTGGCTGCTCTATCGATATCACCAGCGCTGCAGATTCTGAGGCCGTTACGTACCAGAA
GTTGGTGAAAGGACATGCGTACTCTGTCACCGGAGCCGAGGAGGTTGAAAGTTCAGGAAGCCTGCAGAAA
TTGATCCGCATCAGGAATCCCTGGGGACAAGTAGAGTGGACCGGGAAGTGGAATGACAATTGCCCCAGCT
GGAACACGGTTGACCCAGAAGTAAGGGCAAATCTAACCGAACGGCAGGAGGACGGAGAATTCTGGATGTC
CTTCAGTGACTTCCTGAGACACTACTCTCGCCTGGAGATCTGCAACCTGACCCCGGACACCCTCACCTGT
GACTCCTATAAGAAGTGGAAACTCACCAAGATGGATGGGAACTGGAGGCGAGGCTCCACCGCAGGGGGCT
GCAGGAATTACCCAAATACCTTCTGGATGAACCCTCAGTACCTAATTAAGCTGGAGGAAGAAGACGAAGA
TGAGGAGGATGGGGAGAGAGGCTGTACCTTCCTGGTGGGTCTCATCCAGAAGCACCGGCGACGGCAGAGG
AAGATGGGCGAGGACATGCACACCATTGGCTTCGGCATCTATGAGGTTCCAGAGGAGCTAACAGGGCAGA
CCAACATCCACCTCGGCAAAAACTTTTTCCTCACAACCCGAGCCAGGGAGCGGTCAGATACCTTCATTAA
CCTCCGCGAGGTCCTCAACCGCTTCAAGCTGCCCCCGGGAGAGTATGTCCTCGTTCCCTCCACCTTCGAA
CCCCACAAGGATGGCGATTTCTGCATCCGAGTCTTCTCGGAGAAGAAGGCTGACTACCAAGCTGTTGATG
ACGAGATCGAGGCCAACATTGAAGAGATTGACGCCAATGAGGAGGACATTGACGATGGATTCCGAAGGCT
CTTTGTTCAGCTGGCTGGAGAGGATGCAGAGATCTCTGCCTTTGAGCTGCAGACCATCTTAAGAAGAGTT
CTAGCCAAACGCCAAGACATCAAGTCAGACGGCTTCAGCATCGAGACCTGTAAGATCATGGTGGACATGC
TGGATGAAGATGGGAGTGGCAAGCTTGGCCTGAAGGAGTTCTACATCCTCTGGACGAAGATTCAGAAATA
CCAAAAAATCTACCGGGAAATCGATGTGGACAGGTCTGGAACCATGAATTCCTACGAGATGCGGAAAGCA
CTGGAAGAAGCAGGTTTCAAGCTGCCCTGTCAACTTCATCAAGTCATCGTTGCCCGGTTTGCAGACGACG
AGCTAATCATCGACTTTGACAATTTTGTGCGGTGTTTGGTCCGTCTGGAAACGCTATTCAAGATATTCAA
GCAGCTAGACCCTGAGAACACTGGAACGATACAGCTCAACCTTGCTTCGTGGCTGAGTTTTTCAGTACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210127 representing NM_009794
Red=Cloning site Green=Tags(s)

MAGIAIKLAKDREAAEGLGSHERAIKYLNQDYETLRNECLEAGALFQDPSFPALPSSLGYKELGPYSSKT
RGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPPDQSFQENYAGIFH
FQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGG
IAEWYELRKPPPNLFKIIQKALEKGSLLGCSIDITSAADSEAVTYQKLVKGHAYSVTGAEEVESSGSLQK
LIRIRNPWGQVEWTGKWNDNCPSWNTVDPEVRANLTERQEDGEFWMSFSDFLRHYSRLEICNLTPDTLTC
DSYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGERGCTFLVGLIQKHRRRQR
KMGEDMHTIGFGIYEVPEELTGQTNIHLGKNFFLTTRARERSDTFINLREVLNRFKLPPGEYVLVPSTFE
PHKDGDFCIRVFSEKKADYQAVDDEIEANIEEIDANEEDIDDGFRRLFVQLAGEDAEISAFELQTILRRV
LAKRQDIKSDGFSIETCKIMVDMLDEDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKA
LEEAGFKLPCQLHQVIVARFADDELIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIQLNLASWLSFSVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009794
ORF Size 2100 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009794.4
RefSeq Size 3205 bp
RefSeq ORF 2103 bp
Locus ID 12334
UniProt ID O08529
Cytogenetics 1 H5
MW 80.3 kDa
Summary Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (By similarity). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (PubMed:22711986).UniProtKB/Swiss-Prot Function
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Documents
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"NM_009794" in other vectors (4)
SKU Description Size Price
MC202485 Capn2 (untagged) - Mouse calpain 2 (Capn2), (10ug) 10 ug
$642.00
MG210127 Capn2 (tGFP-tagged) - Mouse calpain 2 (Capn2) 10 ug
$789.00 $841.00
MR210127L3 Lenti ORF clone of Capn2 (Myc-DDK-tagged) - Mouse calpain 2 (Capn2) 10 ug
$941.00
MR210127L4 Lenti ORF clone of Capn2 (mGFP-tagged) - Mouse calpain 2 (Capn2) 10 ug
$941.00

Plasmid Map for MR210127

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210127
Circular map for MR210127

file-alt Citations

Distinct Roles for μ-Calpain and m-Calpain in Synaptic NMDAR-Mediated Neuroprotection and Extrasynaptic NMDAR-Mediated Neurodegeneration
Wang, Briz, Chishti et al
J. Neurosci. (2013) 33 (48), 18880-92 DOI: 10.1523/JNEUROSCI.3293-13.2013
AI Summary: In this study, the OriGene m-calpain enzyme was used to assess its role in protein degradation during experiments investigating the distinct contributions of μ-calpain and m-calpain to synaptic and extrasynaptic NMDAR-mediated neuroprotection and neurodegeneration.
Snippet: Equal amounts of proteins (4 μg/μl) were incubated with μ-calpain (Calbiochem) or m-calpain (Origene;both at 2.4 U/ml) in the absence or presence of 2 mm calcium for 30 min at 37°C. Calpain reaction was stopped by adding Laemmli's loading buffer (final concentrations ... Cite SaveSaving
Showing 1-1 of 1 papers.
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