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Cep83 (NM_029852) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210077
Ccdc41 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 41 (Ccdc41)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $635.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cep83
Synonyms 2600001G24Rik; 4921537D05Rik; 5730513H21Rik; Ccdc41
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210077 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACATTTCCTAGCCTATTTCCACCTGGAGGAGATAGTAGGCTGAATCCTGAGCCTGAGTTCCAAA
ATATGTTAATTGATGAAAGGGTACGCTGTGAACATCATAAACATAATTATCAGGCTCTGAAAATTGAACA
CAAAAGGTTGCAGGAAGAATATGTAAAATCACAAAATGAACTTAAACGTGTATTAATTGAAAAGCAAGCA
AGCCAGGAAAAATTCCAACTGCTCCTTGAAGACTTAAGGGGAGAATTAGTAGAGAAAGCTAGAGACATAG
AAAAAATGAAACTGCAGGTACTAACACCACAAAAATTGGAATTGGTAAAAGCCCAACTACAACAAGAATT
AGAAGCTCCAATGCGAGAACGTTTTCGGACTCTTGATGAAGAAGTGGAAAGGTACAGAGCTGAGTATAAC
AAGCTGCGCTACGAGTATACATTTCTCAAGTCAGAGTTTGAACACCAGAAAGAAGAGTTTACTCGGGTTT
CAGAAGAAGAGAAAATGAAATACAAGTCAGAGGTTGCACGACTGGAGAAGGACAAAGAGGAGCTACATAA
CCAGCTGCTTAGTGTTGATCCCACGAGAGACAGCAAACGAATGGAGCAACTTGTTCGAGAAAAAACCCAT
TTGCTTCAGAAATTGAAAAGTTTAGAGGCTGAAGTAGCAGAATTAAGGGCTGAGAAAGAAAATTCTGGTG
CTCAGGTAGAAAATGTCCAAAGAATACAGGTGAGGCAGTTGGCTGAGATGCAGGCTACACTCAGATCCTT
GGAGGCTGAAAAGCAGTCAGCTAAACTACAAGCTGAGCGTTTAGAAAAAGAACTACAATCAAGCAATGAA
CAGAATACCTGCTTAATCAGCAAACTGCATAGAGCTGACCGAGAAATCAGCACACTGGCCAGTGAAGTGA
AAGAGCTTAAACATGCAAACAAACTAGAAATAACTGACATCAAACTGGAGGCAGCAAGAGCTAAGAGTGA
GCTCGAAAGAGAAAGGAATAAGATCCAAAGTGAACTTGATGGATTGCAGTCAGACAATGAAATTCTCAAG
TCAACTGTTGAACACCACAAAGCGCTCTTAGTGGAAAAAGATCGTGAATTAATACGTAAAGTACAAGCTG
CCAAGGAAGAAGGTTACCAGAAACTCATGGTCTTACAAGATGAAAAGCTAGAACTTGAGAACAGATTATC
AGACTTAGAGAAGATGAAGGTGGAGCGTGATGTCTGGAGGCAGTCGGAAAAGGAGCAGTGTGAGGAGAAG
CTGCGGGCCTCCCAGATGGCAGAGGAGGCAGCCCGCAGGGAGCTGCAGAGCACTAGGTTAAAACTTCAAC
AACAAATTGTGAATACCGAAAAAGCAGAAAAAGAAAAACTTGAAAATTCTGAACTGAAACAGCAAATCAG
TCATTTGCAGATCCAAGTGACCTCACTGACACAGTCGGAGAACGACTTACTGAATTCAAACCACATGCTG
AAGGACATGGTGGAGAGACTAAAACAAGAATGCCGAAATTTAAGGAGCCAAGCTGAGAAAGCACAACTAG
ACGTTGAAAAGACACTGGAGGAAAAACAGATCCAGTGGTTGGAAGAAAAGCACAAGCTCCATGAGCGAAT
CACAGACAGAGAAGAAAAGTATAACCAAGCCAAGGAAAAGCTACAGCGCGCAGCGACTGCTCAGAAAAAG
AGAAAATCTCTTCATGAGAACAAATTGAAGAGACTACAGGAGAAAGTAGAAGTCTTGGAGGCAAAGAAAG
AAGAACTAGAAACAGAAAACCAGGTGCTAAATAGACAAAATGTTCCATTTGAAGAATATACAAGGCTTCA
AAAACGACTAAAGGACATACAGAGAAGACATAATGAATTTCGAAGTCTAATTTTGGTTCCTAACATGCCT
CCAACAGCATCGATTAGTCCTGCTAACTTCCAGTCCGCAGTGACAGTTCCTGGAGCGGAACTCTCTTTTC
CCCCTCATCTGCAGGAGGAACAACATCAAAGAGAGCTCTCTCTCCTTCGCAAAAGACTGGAAGAACTGGA
GACTACACAAAGAAAACAACTGGAGGAGCTTGGGTCTCCTGGGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210077 protein sequence
Red=Cloning site Green=Tags(s)

MDTFPSLFPPGGDSRLNPEPEFQNMLIDERVRCEHHKHNYQALKIEHKRLQEEYVKSQNELKRVLIEKQA
SQEKFQLLLEDLRGELVEKARDIEKMKLQVLTPQKLELVKAQLQQELEAPMRERFRTLDEEVERYRAEYN
KLRYEYTFLKSEFEHQKEEFTRVSEEEKMKYKSEVARLEKDKEELHNQLLSVDPTRDSKRMEQLVREKTH
LLQKLKSLEAEVAELRAEKENSGAQVENVQRIQVRQLAEMQATLRSLEAEKQSAKLQAERLEKELQSSNE
QNTCLISKLHRADREISTLASEVKELKHANKLEITDIKLEAARAKSELERERNKIQSELDGLQSDNEILK
STVEHHKALLVEKDRELIRKVQAAKEEGYQKLMVLQDEKLELENRLSDLEKMKVERDVWRQSEKEQCEEK
LRASQMAEEAARRELQSTRLKLQQQIVNTEKAEKEKLENSELKQQISHLQIQVTSLTQSENDLLNSNHML
KDMVERLKQECRNLRSQAEKAQLDVEKTLEEKQIQWLEEKHKLHERITDREEKYNQAKEKLQRAATAQKK
RKSLHENKLKRLQEKVEVLEAKKEELETENQVLNRQNVPFEEYTRLQKRLKDIQRRHNEFRSLILVPNMP
PTASISPANFQSAVTVPGAELSFPPHLQEEQHQRELSLLRKRLEELETTQRKQLEELGSPGE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029852
ORF Size 2076 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029852.2, NP_084128.2
RefSeq Size 3006 bp
RefSeq ORF 2079 bp
Locus ID 77048
UniProt ID Q9D5R3
Cytogenetics 10 C2
MW 82 kDa
Summary Component of the distal appendage region of the centriole involved in the initiation of primary cilium assembly. May collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly.UniProtKB/Swiss-Prot Function
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"NM_029852" in other vectors (2)
SKU Description Size Price
MR210077L3 Lenti ORF clone of Ccdc41 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 41 (Ccdc41) 10 ug
$935.00
MR210077L4 Lenti ORF clone of Ccdc41 (mGFP-tagged) - Mouse coiled-coil domain containing 41 (Ccdc41) 10 ug
$935.00

Plasmid Map for MR210077

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file-download TXT (FASTA format)

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Cloning scheme for MR210077
Circular map for MR210077

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