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Thop1 (NM_022653) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210046
Thop1 (Myc-DDK-tagged) - Mouse thimet oligopeptidase 1 (Thop1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $630.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Thop1
Synonyms AI131655; AI327041; EP24.15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210046 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCCCCCGCAGCCTGTGCTGGGGACGTGGTGGATGCAGCATCCCCAGCCTCCACTGTGAATCATC
TTCGCTGGGATCTGAGCGCACAGCAGATCAGGGCTCTAACCACGCAGCTCATCGAGCAGACCAAGTGTGT
GTACGACCGAGTGGGTGCTCAGAACTTCGAGGATGTGTCGTATGAGAGCACACTGAAGGCACTGGCTGAC
GTGGAGGTCACCTACACAGTGCAGAGGAACATTCTCGACTTCCCCCAACACGTGTCCCCGTGCAAGGACA
TCCGTGCAGCCAGCACAGAGGCTGACAAGAAGCTCTCGGAGTTTGATGTAGAGATGAGCATGAGACAGGA
CGTGTACCAGAGGGTCGTGTGGCTGCAGGAGAAAACCCCGAAAGATTCCCTGAAGCCTGAAGCAGCTCGC
TACCTGGAGCGGCTCATCAAGCTGGGCCGGAGAAACGGCCTCCACTTACCTCAGGACACACAAGAGAAGA
TCAAGAACATCAAGAAGAGGCTGAGCCTGCTGTGCATCGACTTCAACAAGAACCTCAACGAGGACACCAC
CTTCCTGCCCTTCACAAGAGAGGAGCTGGGTGGGCTCCCGGAGGACTTTCTGAGCTCTCTGGAGAAGGCA
GAGGATGGCAAGCTGAAGGTCACCCTCAAGTACCCTCACTACTTCCCGCTGTTGAAGAAGTGCCATGTGC
CAGAGACACGACGCCTGTTGGAGGAGGCCTTCAACTGTCGCTGCAAGGAGGAGAACTGTGCAATTCTGAA
GGAGCTAGTGTCCCTGCGAGCACAGAAGTCCAGCCTGTTGGGGTTCCACACACACGCGGACTACGTCCTG
GAGATGAACATGGCCAAGACCAGTCAAACAGTAGCCACCTTCCTAGATGAGCTGGCACAGAAGCTGAAGC
CCCTGGGTGAGCAGGAGCGCGCGGTCATCCTGGAGCTGAAGGAGGCAGAGTGCGCCAAGCGGGGACTGCC
ATTCGACGGGCGCATCCACGCATGGGACATGCGCTACTACATGAACCAGGTGGAGGAGACTCGCTATCGT
GTGGACCAGAACCTGCTGAAGGAGTACTTCCCCATGCAGGTGGTCACACGTGGGCTGCTGGCCATCTACC
AAGAGCTGCTGGGGCTGACCTTCACGCTGGAGGAAGGTGCCGCTGCCTGGCACGAGGACGTGCGGCTCTA
CTCAGTGCGGGACGCCGCCTCTGGAGAGGAGATCGGCAAGTTCTACCTCGACCTATACCCCAGGGAAGGG
AAATATGGCCATGCAGCCTGCTTTGGCCTGCAGCCTGGCTGCCTACGGCAGGATGGCAGCCGGCAGCTGG
CCGTCGCAGCCATGGTGGCCAATTTCACGAAGCCCACACCTGACGCGCCGTCCCTGCTGCAGCATGATGA
GGTGGAGACCTACTTCCACGAGTTCGGGCACGTCATGCACCAGCTGTGCTCGCAGGCGGAGTTTGCCATG
TTCAGTGGGACCCACGTGGAGCGGGACTTTGTGGAGGCGCCGTCCCAGATGCTGGAGAACTGGGTGTGGG
AGAAGGAGCCCCTGATGCGCATGTCTCAGCACTACCGCACGGGCAGCGAGGCCCCTCAGGACCTCCTGGA
GAAGCTCATCAAGTCTCGCCAGGCCAATGCAGGTCTCTTCAACCTGCGGCAGATTGTGCTTGCCAAGGTG
GATCAGGTCCTGCACACACAGACAGACGCAGACCCGGCTGAGGAATATGCCCGGCTCTGCCAAGAGATCC
TCGGGGTGCCAGCCACACCAGGTACCAACATGCCAGCCACTTTCGGCCACCTAGCTGGCGGCTATGATGC
TCAGTACTATGGCTACTTATGGAGCGAAGTGTACTCGATGGACATGTTCCACACACGCTTCAAGCAAGAG
GGTGTGCTTAGCCCCAAGGTTGGCATGGACTACAGGACCAGCATCCTGAGGCCAGGGGGCTCTGAGGACG
CCAGCGCCATGCTGAAGCAGTTCCTGGGCCGCGACCCCAAGCAGGACGCCTTCCTCCTGAGCAAGGGTCT
GCAGGTGGAGGGCAGCGAGGCGCCTGCGTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210046 protein sequence
Red=Cloning site Green=Tags(s)

MKPPAACAGDVVDAASPASTVNHLRWDLSAQQIRALTTQLIEQTKCVYDRVGAQNFEDVSYESTLKALAD
VEVTYTVQRNILDFPQHVSPCKDIRAASTEADKKLSEFDVEMSMRQDVYQRVVWLQEKTPKDSLKPEAAR
YLERLIKLGRRNGLHLPQDTQEKIKNIKKRLSLLCIDFNKNLNEDTTFLPFTREELGGLPEDFLSSLEKA
EDGKLKVTLKYPHYFPLLKKCHVPETRRLLEEAFNCRCKEENCAILKELVSLRAQKSSLLGFHTHADYVL
EMNMAKTSQTVATFLDELAQKLKPLGEQERAVILELKEAECAKRGLPFDGRIHAWDMRYYMNQVEETRYR
VDQNLLKEYFPMQVVTRGLLAIYQELLGLTFTLEEGAAAWHEDVRLYSVRDAASGEEIGKFYLDLYPREG
KYGHAACFGLQPGCLRQDGSRQLAVAAMVANFTKPTPDAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAM
FSGTHVERDFVEAPSQMLENWVWEKEPLMRMSQHYRTGSEAPQDLLEKLIKSRQANAGLFNLRQIVLAKV
DQVLHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQE
GVLSPKVGMDYRTSILRPGGSEDASAMLKQFLGRDPKQDAFLLSKGLQVEGSEAPAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022653
ORF Size 2061 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022653.1, NM_022653.2, NM_022653.3, NM_022653.4, NP_073144.2
RefSeq Size 2585 bp
RefSeq ORF 2064 bp
Locus ID 50492
UniProt ID Q8C1A5
Cytogenetics 10 39.72 cM
MW 78 kDa
Summary Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments (By similarity).UniProtKB/Swiss-Prot Function
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"NM_022653" in other vectors (4)
SKU Description Size Price
MC202864 Thop1 (untagged) - Mouse thimet oligopeptidase 1 (Thop1), (10ug) 10 ug
$630.00
MG210046 Thop1 (tGFP-tagged) - Mouse thimet oligopeptidase 1 (Thop1) 10 ug
$830.00
MR210046L3 Lenti ORF clone of Thop1 (Myc-DDK-tagged) - Mouse thimet oligopeptidase 1 (Thop1) 10 ug
$930.00
MR210046L4 Lenti ORF clone of Thop1 (mGFP-tagged) - Mouse thimet oligopeptidase 1 (Thop1) 10 ug
$930.00

Plasmid Map for MR210046

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210046
Circular map for MR210046

file-alt Citations

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