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Lrch4 (NM_146164) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209990
Lrch4 (Myc-DDK-tagged) - Mouse leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $624.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lrch4
Synonyms 2810008P14Rik; 2900069C24Rik; AI558103; LRN; LRRN4; mFLJ00248; SAP25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209990 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGCAGTAGCGGGCCCACTCGCCGCCGGGGGTGAGGAAGCTGCAGCTTCAGTGTCCTTGCCAG
GGTCTCCTGGTCTACCTGGGAGCCGTAGCGCAGAACGAGCCCTAGAGGAGGCTGTGGCCACCGGGACCCT
GAACTTGTCCAACCGGCGTTTGAAGCACTTCCCCCGGGGCGCGGCCCGCAGTTACGACTTGTCAGACATC
ACCCAGGCTGACTTGTCTCGGAACCGGTTTCCCGAGGTGCCTGAGGCAGCTTGCCAGCTGGTGTCCCTGG
AAGGCCTGAGCCTCTACCACAATTGCCTGAAATGCCTGAACCCAGCCTTGGGGAATCTTACAGCCCTCAC
CTACCTCAACCTCAGCCGGAACCAGCTGTCGTCGTTGCCACCCTACATCTGCCAGCTGCCCCTTCGAGTG
CTTATCATCAGCAACAACAAGTTAGGAGCCCTGCCTCCAGACATCAGCACCTTGGGAAGCCTGCGGCAGC
TTGATGTGAGCAGCAATGAGCTGCAGTCCCTGCCCGTGGAGCTGTGTAGCCTCCGTTCCCTGCGGGATCT
CAATGTTCGAAGGAACCAGCTCAGTACCCTGCCTGATGAGCTGGGAGACCTTCCTCTGGTCCGCCTGGAT
TTCTCCTGTAACCGCATCTCCCGAATCCCCGTCTCCTTCTGCCGCCTCAGGCACCTGCAGGTCGTTCTGC
TGGATAGCAACCCCCTACAGAGTCCACCTGCCCAGATATGCCTGAAGGGGAAACTTCACATCTTCAAGTA
CCTAACAATGGAAGCTGGCCGGAGGGGAGCCGCCCTCGGGGACCTGGTCCCTTCCCGCCCCCCAAGTTTT
AGTCCTTGCCCTGCCGAAGATTTATTTCCGGGACGTCGTTATGATGGTGGCCTGGACTCAGGCTTCCACA
GCGTTGACAGTGGCAGCAAGAGGTGGTCAGGAAATGAGTCCACAGATGATTTTTCAGAGCTGTCTTTCCG
GATCTCGGAGCTGGCTCGTGATCCCCGGGGGCCTAGACAACCTAGGGAAGATGGCGCTGGCGATGGAGAC
CTGGAGCAGATTGACTTTATTGACAGCCACGTTCCTGGGGAAGATGAAGATCGAAGTGCAGCTGAGGAGC
AGCTGCCTTCTGAATTAAGCCTTGTAGCAGGGGATGTGGAGAAGCCATCTAGCAGCAGGCGAGAGGAGCC
TGCAGGGGAGGAGAGGCGGCGCCCAGACACTTTGCAGTTGTGGCAGGAACGGGAGCGGAAGCAACAGCAA
CAGAGTGGGGGATGGGGGTCCCCCAGGAAGGACAGCGTCCTGAAGCGGGGGATCCGAGCTGCCGGGGCAG
GTGCTTCGGCCCCATCCACACAGGCCACCTGCAATGGCCCACCAAAGTCCAGCACTACCCAACTGGGAGT
TTCAGGGGGGCAGGGAGCTCCCACACCACCCCCCACCTCCCAGGACCCCCTTCCTGTATCTGGACCAGTG
ACAGCTCCTGTTCCCAGGCCCCTGGGCTCCATTCAGAGACCAAACAGCTTCCTCTTCCGGTCCTCTTCTC
AGAGTGGCTCTAGTCCTTCCTCTCCAGAGTCTGTTTTGAGACCTCGGCCGTTTCCTCAGGAGAAGGAGCT
AATATCCCAACTTCGCCAGGTCCTAGAGTCGCGGCTGCAGCAGCCCCTACCTGAGGACCTGGCAGAAGCT
CTCGCCAACGGAGTCCTCCTTTGCCAGCTGGCGAACCAGCTGCGGCCCCGCTCTGTACCCTTCATTCATG
TGCCCTCACCTGCTGTGCCAAAGCTCAGTGCTCTCAAGTCTCGGAAGAATGTCGAGAGTTTCCTAGAAGC
CTGTCGGAAAATGGGTGTGCCTGAGGCTGACCTGTGCTCGCCCTCGGATCTCCTCCGGGGCACCGCCCAG
GGGCTGCAGACCGTCCTGGAGGCTGTGATCCTGGTTGGGGGCAAGGCCCCTCTCCCAGTCCAGCCCTCCT
CTGGTCTGGGTGGCTTCCTCCTCTTCTACGTGGTCTTCATGCTGCTGCTCTATGTCGTCTACACTCGGCT
CCTGGGCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209990 protein sequence
Red=Cloning site Green=Tags(s)

MAAAVAGPLAAGGEEAAASVSLPGSPGLPGSRSAERALEEAVATGTLNLSNRRLKHFPRGAARSYDLSDI
TQADLSRNRFPEVPEAACQLVSLEGLSLYHNCLKCLNPALGNLTALTYLNLSRNQLSSLPPYICQLPLRV
LIISNNKLGALPPDISTLGSLRQLDVSSNELQSLPVELCSLRSLRDLNVRRNQLSTLPDELGDLPLVRLD
FSCNRISRIPVSFCRLRHLQVVLLDSNPLQSPPAQICLKGKLHIFKYLTMEAGRRGAALGDLVPSRPPSF
SPCPAEDLFPGRRYDGGLDSGFHSVDSGSKRWSGNESTDDFSELSFRISELARDPRGPRQPREDGAGDGD
LEQIDFIDSHVPGEDEDRSAAEEQLPSELSLVAGDVEKPSSSRREEPAGEERRRPDTLQLWQERERKQQQ
QSGGWGSPRKDSVLKRGIRAAGAGASAPSTQATCNGPPKSSTTQLGVSGGQGAPTPPPTSQDPLPVSGPV
TAPVPRPLGSIQRPNSFLFRSSSQSGSSPSSPESVLRPRPFPQEKELISQLRQVLESRLQQPLPEDLAEA
LANGVLLCQLANQLRPRSVPFIHVPSPAVPKLSALKSRKNVESFLEACRKMGVPEADLCSPSDLLRGTAQ
GLQTVLEAVILVGGKAPLPVQPSSGLGGFLLFYVVFMLLLYVVYTRLLGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146164
ORF Size 2040 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146164.2
RefSeq Size 3081 bp
RefSeq ORF 2043 bp
Locus ID 231798
UniProt ID Q921G6
Cytogenetics 5 G2
MW 73.2 kDa
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Documents
Resources
"NM_146164" in other vectors (4)
SKU Description Size Price
MC200859 Lrch4 (untagged) - Mouse leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 1, (10ug) 10 ug
$624.00
MG209990 Lrch4 (tGFP-tagged) - Mouse leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4) 10 ug
$824.00
MR209990L3 Lenti ORF clone of Lrch4 (Myc-DDK-tagged) - Mouse leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 1 10 ug
$924.00
MR209990L4 Lenti ORF clone of Lrch4 (mGFP-tagged) - Mouse leucine-rich repeats and calponin homology (CH) domain containing 4 (Lrch4), transcript variant 1 10 ug
$924.00

Plasmid Map for MR209990

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209990
Circular map for MR209990

file-alt Citations

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