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1200016B10Rik (BC055064) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209983
1200016B10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1200016B10 gene (cDNA clone MGC:62864 IMAGE:6528686)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $622.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1200016B10Rik
Synonyms 1200016B10Rik; C1orf26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209983 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTTTGTACTACCAGTGCTCACAAGCTTGAGACTGTTTATGACCATAAGTTTCTAAAGATGTCTA
TCAAAGAATCTTGTGCAAAGGAAGAAAAGTCTCAGAAAAAACAGACTATCTCTTCACCAACTTTTAATGA
AGACAAGAAAAAGGGAGAAATCTCAGCAGGTTCTACTAGTTCAGAACATGGTGTTCAGCCTGTTTCAACC
AAGAAGAGAAAACTGAAAGCAGATGATACAGACAATGTAGACTATAATGCAAACAGAAAAAACAGTAAAA
GATTGAATGTAGAAGTTTTCATTCCTAAAAAGAGACTAAAATTCAGTTCTTCGACCCAAGCGGTATCATA
TTTAAATAATAATCAAATGACTTCACACAGTTGTTCATCAAATGGAACTAAAGATACTAAAGTTAAAGAT
TGTAAATTGACAAATATTGGAAGTAAGCTTAATTATGAAATTAAAAACCATAGCCGCATTAAAATTACCA
AAGATATGAAATCTAAACCTGTAGACCAGACAAAGGAAAAGAATTGGCCTTCTTTGCTTATTCAGAAGAA
GATGAAAGAACTCAAGAAAGAAAAAAATAATAAAGACAGTTCTGAAGAATTGGAGAAATGCAAGAAAAAT
CACCTTCCTCAGAATTATAATTTTAGCAATATGATCAAGGAATCATTTGAATCAGGAAGAAAGAAGATTA
GCTTCAAAATTCCAAAAAAATCCTCTACTACCCTCCAGAAGCTTGTCGAAGAAAAGATCTTTACTATAGA
TTCTAGCAAGTCAAAGAGTAAGCAGGAGGAGAAGCAACACTTGCAAAGTCACCAGATGTCATTAAATCTG
GCCAGGCACAAAACTGAAAACTCATTTTCAGATTCTACACATAAGCAGTCTGTTTGTGAGAGGATAAGAA
AGGACCATTGTGAGCACCAAGGAACGAGTAGTTCCGATTCTAAGGAAAACCATGCCCAGAGTTTTGAAAC
ACCATGTTCTTCAGTGTCATCTGAGGATACCCAAGATGCAGATGAAGAGATGCAGATAGTAGAAGAGCTC
CACGCTGCACGTGTCGGGAGAAGCGTGGATTTGCCTGTGGTTCCACCGTCTGTAGAGCTCATGAGCATGG
AGATTGACCTGGTGGATGAGGACGCACATCCCTCTGCTGCACACACTGCCTCAGACACGAAGCTTCTCAT
TGTTATGGACACAAATGTTCTCATGAATCATCTCAAATTCGTTAAAATTTTGAAGACGACAGAAATACCA
GGATTTGACACACTTGTGTTAATAATTCCTTGGGTGGTTATACAAGAGCTAGATCGTATGAAGGCAGGAA
AATTACTAAAACATGTTCAGCATAAAGCTGTACCTGCAATTCATTTTATCAACAACAGCCTCAAAAGTCA
AGACAGAAAGCTGTGGGGCCAGTCATTACAGCTGGCATCCCAAAAACTTTATGGACTGAGTGACGAGAAC
AATGATGACCGAGTATTAAAGTGCTGTCTTCAGTACCAGCAATTATTCCCGTGTTCTCTTGTTATTCTGT
GCACGGATGATAGAAATTTAAGAACTAAAGGCCTGATAAGTGGTGTGAAGTCCCTCAGTAAAGAAGATCT
GGATACAGAGATATTAAATTTAACTCTGAACACAGATTTGTGTCATCAGCCTTGTATTTCTAAGCAGCAG
TTGAACGCAGAAGTACCACCTGCGAAAGAGACTCCTAAGGAAGGAGCTGCAAATTCCGGACTGTCCATTC
TACTGGAGCGGATCATTTGTGATCTTGAAAAATGCCTTGGGACAGCTTTATCTTCAATATTAGAAACAGA
AATGAAAATTGCTTTTGGAAATCTTTGGATGGAGGTGCTGTATTTGAAACCACCATGGACTCTAATAAAT
CTATTACAGTGCTTTAAAAAACATTGGCTGGCTGTATTTGGATTAGTTATGGAAAATAACTTGCTTGTAA
CTGTTGAGAGCCTATATGAAAATCTCTGTAAAGGTCAAGACAAAACTGAAGCCAAGTTCTTCAGAAAGCA
CAAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209983 protein sequence
Red=Cloning site Green=Tags(s)

MLLCTTSAHKLETVYDHKFLKMSIKESCAKEEKSQKKQTISSPTFNEDKKKGEISAGSTSSEHGVQPVST
KKRKLKADDTDNVDYNANRKNSKRLNVEVFIPKKRLKFSSSTQAVSYLNNNQMTSHSCSSNGTKDTKVKD
CKLTNIGSKLNYEIKNHSRIKITKDMKSKPVDQTKEKNWPSLLIQKKMKELKKEKNNKDSSEELEKCKKN
HLPQNYNFSNMIKESFESGRKKISFKIPKKSSTTLQKLVEEKIFTIDSSKSKSKQEEKQHLQSHQMSLNL
ARHKTENSFSDSTHKQSVCERIRKDHCEHQGTSSSDSKENHAQSFETPCSSVSSEDTQDADEEMQIVEEL
HAARVGRSVDLPVVPPSVELMSMEIDLVDEDAHPSAAHTASDTKLLIVMDTNVLMNHLKFVKILKTTEIP
GFDTLVLIIPWVVIQELDRMKAGKLLKHVQHKAVPAIHFINNSLKSQDRKLWGQSLQLASQKLYGLSDEN
NDDRVLKCCLQYQQLFPCSLVILCTDDRNLRTKGLISGVKSLSKEDLDTEILNLTLNTDLCHQPCISKQQ
LNAEVPPAKETPKEGAANSGLSILLERIICDLEKCLGTALSSILETEMKIAFGNLWMEVLYLKPPWTLIN
LLQCFKKHWLAVFGLVMENNLLVTVESLYENLCKGQDKTEAKFFRKHKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC055064
ORF Size 2037 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC055064, AAH55064
RefSeq Size 2865 bp
RefSeq ORF 2039 bp
Locus ID 66875
Cytogenetics 1 G1
MW 77.1 kDa
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"BC055064" in other vectors (4)
SKU Description Size Price
MC220653 1200016B10Rik (untagged) - Mouse RIKEN cDNA 1200016B10 gene (cDNA clone MGC:62864 IMAGE:6528686), (10ug) 10 ug
$685.00
MG209983 1200016B10Rik (tGFP-tagged) - Mouse RIKEN cDNA 1200016B10 gene (cDNA clone MGC:62864 IMAGE:6528686) 10 ug
$822.00
MR209983L3 Lenti ORF clone of 1200016B10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1200016B10 gene (cDNA clone MGC:62864 IMAGE:6528686) 10 ug
$922.00
MR209983L4 Lenti ORF clone of 1200016B10Rik (mGFP-tagged) - Mouse RIKEN cDNA 1200016B10 gene (cDNA clone MGC:62864 IMAGE:6528686) 10 ug
$922.00

Plasmid Map for MR209983

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209983
Circular map for MR209983

file-alt Citations

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