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Taf6 (NM_009315) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209976
Taf6 (Myc-DDK-tagged) - Mouse TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $621.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Taf6
Synonyms 80kDa; AW549759; p80; TAF(II)80; Taf2e; TAFII70
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209976 representing NM_009315
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGAGAAGAAACTGAAACTCAGCAATACTGTGTTGCCCTCTGAGTCCATGAAGGTGGTGGCAG
AATCTATGGGCATTGCTCAGATTCAGGAGGAGACTTGCCAGCTACTGACAGATGAGGTCAGCTACCGCAT
CAAGGAGATTGCCCAGGATGCCCTGAAGTTCATGCACATGGGGAAACGACAGAAGCTCACTACCAGTGAC
ATTGACTATGCATTGAAGTTAAAGAATGTGGAGCCACTGTATGGCTTCCATGCTCAGGAGTTCATCCCTT
TCCGGTTTGCCTCTGGCGGGGGCCGGGAGCTGTACTTCTATGAAGAGAAGGAAGTGGACCTGAGTGACAT
CATCAACACCCCTCTGCCTCGGGTGCCTCTGGATGTCTGCCTCAAAGCTCATTGGCTAAGCATCGAAGGC
TGCCAGCCAGCTATACCAGAGAATCCACCCCCAGCTCCCAAAGAGCAGCAGAAGGCTGAGGCCACAGAAC
CCCTGAAATCAGCAAAGCCAGGCCAAGAAGAAGATGGACCCCTGAAAGGCAAAGGTCAAGGGGCAGCTGC
AGCTGATGGCAAAGGAAAAGAGAAGAAGGCACCTCCCTTGCTGGAGGGGGCCCCTTTCCGCCTGAAGCCC
CGCAGCATCCATGAGCTGTCTGTGGAGCAGCAGCTGTATTACAAGGAGATCACGGAAGCCTGTGTGGGAT
CCTGTGAGGCCAAGAGAGCGGAAGCTCTGCAGAGCATTGCCACGGACCCAGGGCTCTATCAGATGCTACC
ACGGTTCAGTACCTTCATCTCAGAGGGGGTCCGTGTGAATGTAGTTCAGAATAACCTGGCCTTGCTTATC
TACCTGATGCGTATGGTGAAGGCCCTGATGGACAACCCCACACTGTATCTAGAGAAATATGTGCATGAGC
TCATTCCAGCAGTAATGACCTGCATTGTGAGTAGACAGCTGTGCTTGCGGCCAGATGTGGACAATCACTG
GGCACTCCGGGATTTTGCTGCCCGTCTAGTGGCCCAAATCTGTAAGCATTTCAGCACAACCACCAACAAC
ATCCAATCCCGAATCACTAAGACCTTTACCAAGAGCTGGGTCGACGAGAAGACTCCATGGACAACTCGAT
ATGGCTCCATTGCAGGCCTAGCAGAGCTGGGACATGATGTGATTAAGACGCTAATTCTGCCAAGGCTGCA
GCAGGAAGGGGAACGGATCCGCAGTGTCTTGGATGGGCCTGTGCTGAGCAACATCGACCGTATTGGAGCA
GACCATGTGCAGAGCCTTCTCCTGAAACACTGTGCCCCAGTACTGGCCAAGCTGCGCCCTCCACCTGACA
ATCAGGATGCCTACCGGGGAGAATTTGGGTCCCTTGGGCCCCTTCTCTGTTCCCATGTGGTGAAGGCCAG
GGCCCAGGCTGCTCTACAAGCTCAGCAGGTCAACAGGACCACACTCACCATCACACAGCCTCGGCCCACA
CTGACCCTTTCACAGGCCCCTCAGCCAGGCCCTCGCACGCCTGGCTTATTGAAGGTCCCTGGCTCCATTG
CCTTGCCTGTTCAGACACTGGTGTCTGCGAGAGCTGCTGCTCCTCCTCAGCCTTCTCCTCCTCCAACCAA
GTTTATTGTAATGTCCTCATCTTCCAGTGCCTCATCAACCCAGCAGGTTCTGTCCCTCAGCACCTCAGCT
CCTGGCTCAGGCTCTACTACTACCTCTCCTGTCACCACCACGGTTCCCAGCGTGCAGCCCATCGTCAAGC
TGGTCTCCACTGCTACCACTGCACCCCCAAGCACAGCCCCTGCTGGTTCTGGCAGTGTTCAGAAATACAT
TGTGGTCTCTCTTCCCCCAACAGGGGAAGGCAAAGGAGGCCCTCCTTCTCATCCTTCTCCAGTCCCCCCT
TCATCTTCTTCACCATCCCCACTTGGGGGAAGTACCCTCTGTGGGGGGAAGCAGGAAGCTGGGGACAGCC
CCCCTCCAGCTCCAGGGACTCCAAAGGCTAATGGCTCGCAGCCTACTGGACCTGGCTCTCCCCAGCCTGC
TCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209976 representing NM_009315
Red=Cloning site Green=Tags(s)

MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHMGKRQKLTTSD
IDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINTPLPRVPLDVCLKAHWLSIEG
CQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKGKGQGAAAADGKGKEKKAPPLLEGAPFRLKP
RSIHELSVEQQLYYKEITEACVGSCEAKRAEALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLI
YLMRMVKALMDNPTLYLEKYVHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNN
IQSRITKTFTKSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGA
DHVQSLLLKHCAPVLAKLRPPPDNQDAYRGEFGSLGPLLCSHVVKARAQAALQAQQVNRTTLTITQPRPT
LTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIVMSSSSSASSTQQVLSLSTSA
PGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPAGSGSVQKYIVVSLPPTGEGKGGPPSHPSPVPP
SSSSPSPLGGSTLCGGKQEAGDSPPPAPGTPKANGSQPTGPGSPQPAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009315
ORF Size 2034 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009315.4
RefSeq Size 2264 bp
RefSeq ORF 2037 bp
Locus ID 21343
UniProt ID Q62311
Cytogenetics 5 G2
MW 72.7 kDa
Summary TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009315" in other vectors (4)
SKU Description Size Price
MC220328 Taf6 (untagged) - Mouse TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf6), (10ug) 10 ug
$684.00
MG209976 Taf6 (tGFP-tagged) - Mouse TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf6) 10 ug
$821.00
MR209976L3 Lenti ORF clone of Taf6 (Myc-DDK-tagged) - Mouse TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf6) 10 ug
$921.00
MR209976L4 Lenti ORF clone of Taf6 (mGFP-tagged) - Mouse TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf6) 10 ug
$921.00

Plasmid Map for MR209976

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209976
Circular map for MR209976

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