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Ski (BC068305) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209957
Ski (Myc-DDK-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $618.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ski
Synonyms MGC8300
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209957 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGCTGCAGCGGCCGGCCGGGGCGGCTTCCAGCCGCACCCGGGGCTGCAGAAGACGCTGGAGCAGT
TCCACCTGAGCTCCATGAGCTCGCTGGGCGGCCCGGCCGCTTTCTCGGCGCGCTGGGCGCAGGAGGCCTA
CAAGAAGGAGACCGTGCTGGAAGGGGAGACCATCTCTTGTTTCGTGGTGGGAGGTGAGAAGCGTCTGTGC
CTGCCGCAGATCCTCAACTCGGTGCTGCGCGACTTCTCACTGCAGCAGATCAACTCCGTGTGCGATGAGC
TGCACATCTACTGCTCGCGCTGCACCGCCGACCAGCTGGAGATCCTCAAAGTCATGGGCATCCTGCCCTT
CTCCGCGCCCTCCTGCGGGCTCATCACCAAGACGGACGCCGAGCGCCTGTGCAACGCGCTGCTCTACGGC
GGCGCCTACCCGCCGCCTTGCAAGAAGAAGTTGGCGGCCAGCCTGGCGCTGGGCCTGGAGCTCAGCGAAC
GCAGCGTCCGCGTGTACCACGAGTGCTTCGGCAAGTGCAAAGGGCTGCTGGTGCCCGAGCTGTACAGCAG
TCCCAGCGCTGCCTGCATCCAGTGCCTCGACTGCCGCCTCATGTACCCGCCGCACAAGTTCGTTGTACAC
TCACACAAGGCCCTGGAGAACCGGACCTGCCACTGGGGCTTCGACTCAGCCAACTGGCGGGCCTACATCC
TGCTGAGCCAGGACTACACGGGCAAGGAGGAGCAGGCCCGCCTTGGCCGCTGCCTGGACGACGTGAAGGA
GAAGTTCGACTATGCCAACAAGTACAAGCGGAGGGTGCCCCGGGTCTCAGAGCCCCCAGCTTCCATAAGA
CCCAAAACAGACGACACTTCCTCACAGTCCCCTGCGTCTTCTGAGAAGGACAAGCAGTCCACTTGGCTGC
GGACCTTGGCTGGTTCCTCCAATAAGAGCCTCGGCTGCACTCACCCTCGCCAGCGCCTTTCTGCTTTCCG
GCCCTGGTCCCCTGCAGTGTCTGCGAGTGAGAAAGAGACGTCCCCACACCTCCCAGCTTTGATTCGAGAC
AGCTTCTACTCCTACAAGAGCTTTGAGACAGCCGTGGCCCCCAATGTGGCCCTAGCACCACCAACCCAGC
AAAAGGTTGTGAACAGTCCACCCTGCACCACCGTGGTCTCCCGGGCACCAGAGCCTCTTACTACTTGTAT
CCAGCCACGAAAACGGAAGCTGACTCTGGACACAGCAGGAGCCCCAGACATGCTGACACCTGTGGCTGCT
GCAGAGGAAGACAAGGATTCTGAGGCCGAGGTTGAAGTTGAAAGCAGAGAGGAGTTCACCTCCTCCTTGT
CCTCGCTGTCCTCTCCGTCCTTTACCTCGTCCAGCTCTGCCAAGGATCTAAGCTCCCCAGGCATGCATGC
TCCGCCCGTGGTTGCCCCCGATGCTGCAGCCCATGTTGATGCCCCAAGTGGGCTGGAGGCAGAGTTGGAG
CACCTGCGGCAAGCCCTGGAGGGTGGCCTGGATACTAAGGAAGCCAAAGAAAAGTTCCTGCATGAGGTGG
TCAAAATGCGAGTTAAGCAAGAGGAAAAGCTGACAGCAGCCCTGCAGGCTAAGCGCAGCCTGCACCAGGA
GCTAGAATTCTTGCGTGTGGCCAAGAAGGAGAAACTGCGGGAAGCCACAGAGGCCAAGCGCAGTTTGAGG
AAAGAGATTGAGCGGCTCCGAGCTGAAAACGAGAAGAAGATGAAAGAGGCCAACGAGTCCCGGGTGCGCC
TGAAGCGAGAGCTGGAGCAGGCGCGGCAGGTCCGGGTGTGTGACAAGGGCTGCGAGGCCGGGCGCCTGCG
TGCCAAGTACTCAGCCCAGATTGAGGACCTGCAAGCGAAGCTGCAGCATGCGGAGGCCGACCGCGAGCAG
CTTCGAGCTGACCTGCTGCGGGAACGTGAGGCCCGCGAGCATCTGGAGAAGGTGGTGAGGGAGCTTCAGG
AGCAGCTGCGGCCTCGGCCACGGCCTGAGCATCCTGGAGGTGAGAGCAACGCTGAGCTGGGTCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209957 protein sequence
Red=Cloning site Green=Tags(s)

MEAAAAGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKETVLEGETISCFVVGGEKRLC
LPQILNSVLRDFSLQQINSVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYG
GAYPPPCKKKLAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVH
SHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYANKYKRRVPRVSEPPASIR
PKTDDTSSQSPASSEKDKQSTWLRTLAGSSNKSLGCTHPRQRLSAFRPWSPAVSASEKETSPHLPALIRD
SFYSYKSFETAVAPNVALAPPTQQKVVNSPPCTTVVSRAPEPLTTCIQPRKRKLTLDTAGAPDMLTPVAA
AEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLSSPGMHAPPVVAPDAAAHVDAPSGLEAELE
HLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLTAALQAKRSLHQELEFLRVAKKEKLREATEAKRSLR
KEIERLRAENEKKMKEANESRVRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQAKLQHAEADREQ
LRADLLREREAREHLEKVVRELQEQLRPRPRPEHPGGESNAELGP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC068305
ORF Size 2025 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC068305, AAH68305
RefSeq Size 3912 bp
RefSeq ORF 2027 bp
Locus ID 20481
Cytogenetics 4 86.17 cM
MW 75 kDa
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"BC068305" in other vectors (4)
SKU Description Size Price
MC220756 Ski (untagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626), (10ug) 10 ug
$681.00
MG209957 Ski (tGFP-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626) 10 ug
$818.00
MR209957L3 Lenti ORF clone of Ski (Myc-DDK-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626) 10 ug
$918.00
MR209957L4 Lenti ORF clone of Ski (mGFP-tagged) - Mouse Sloan-Kettering viral oncogene homolog (cDNA clone MGC:76530 IMAGE:30093626) 10 ug
$918.00

Plasmid Map for MR209957

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209957
Circular map for MR209957

file-alt Citations

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