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Nfe2l3 (NM_010903) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209844
Nfe2l3 (Myc-DDK-tagged) - Mouse nuclear factor, erythroid derived 2, like 3 (Nfe2l3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $615.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nfe2l3
Synonyms Nrf3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209844 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCTCCCGAAGCCTTGGTGGGCGGGCGGCGGCCTCCTGCAACTCACGATTCTGTTGAGCTTGGTAG
GGCTCCGTGTGGACCTGGACCTCTTCCTGCCGCCGCCGGCCGCCGCACTTTGGGAAGAGCTGCTCCCACT
CTGCCCCACCCGCCCCGCCTCGGCTTCTAACCCCTTCTCGGCTTCGGAAGGCTGGGAACGCACCCCCCTG
CTACCTGCCAAAGGCCGGCTGCTGCACGAAGTGCGCGCGCTCGGGGTCCCCTTCATTCCCCGCACCCGCG
TGGACGCGTGGCTAGTGCACAGCGTGGCGACTGGGAACGCAGACGGGGCCCACGGGCTGCTCGGCACCGC
CGCCTCGTCGGCCGTGGGAGACGGCGGCCAGTCAGCATCCGCGGGCGGCGGGGACCCCCGAGCGGCTCAT
AGCAGCCCTCTGGCCGCTGAGGAAGAGGAGGAGAAGGCGGCCGAACCGACGGCGCAGGTGCCAGATGCAG
GCGGATGCGGGAGCCAGGAGAACGGGATGCTAAGAGAAAAGAGCGAAGCTGTGGATCACAGTTCCCAGCA
GGAGGAAAACGAGGAAGGGGTGTCAGCCCAGGCAAAATCGCGACAGCAGAGTAAGACGGAGGAACACAAA
ATGGCGTGTGCCTCTGCCAGGGAAGCCGAGAAGATCACCGAGGCTAGGAACGAGAGCCATCTACATTGGT
CAGATACATCTTTCTCTTTGGAAGATTTATTCCAACTACTTTCATCACAGCCTGAACATTCTCTGGAGGG
CATCTCAGTGGAAGACATTCCACCTTTTCTGAGCAGTGTCTGTGAAAGTGTTAATTCCTCAGCACAGAAC
ATAAATTTGAGCCAAGCTATAAGCCATGATGTCAATCTTCATGAAGCCATGCTGTTGTACCCCAACAACA
CATTTAGAAGAGATCCATCAGCAAGGTCTTCACAGGCACAAGAACCATTTCTGCAGTTAAATCCTCATAC
AAATCCTGAGCAAGCCACTCCTGCAATGTCTCTTCCACCGTTTGACAATCAAATGAGGAACCTAACAAGC
CAAGATCTCCTGTATGACCTGGATTCAAATATATTTGATGGAATAAACCTAATGTCATTGGCCACAGGTT
TCAGTCCATTGGAAGTTTCTCAACTTTTTGAAGAACCAGATTCCGGGCTCTCATTAAATTCAAGTTACAA
CAGCACCTCTCTCACAAATTCTTACCGTATTTATGATGGGACTGTAGGTTACAATAGCGACCTACAGTCT
CTTTGTCATGATTTAGGTGCTGTAGGAGGCTGCTACCCAGAACCCCATAAACATTGTCATATGGACCATA
GGACTATAGCTGGCTTTCATGTGTCCCTTGAATGTCAGCAAGTATTTCATGACCACACTTACCATTTACA
GTCAGGTGCATCAGAGCCCACATCTGAATCTTTTGCATGGTCTGAGAAGTCGCAGAAAACCAGTGGATGC
CTTGATAACCCAGATAGAAACTTGAGCCGTGATGAACAGCGTGCTAAAGCTCTGCACATTCCCTTTTCTG
TAGATGAAATTGTCCGTATGCCTGTTGACTCTTTCAACAGCATGTTAAGCAGATACTACCTGACAGACTT
ACAAGTGTCTCTCATCCGTGATATCAGGCGAAGAGGAAAAAATAAAGTTGCTGCTCAGAACTGCCGTAAA
CGTAAACTAGACATCATTTTGAATCTAGAGGATGATATATGTAACTTGCAAGCAAAGAAAGAAGCCCTTA
AAAATGAGCAAACTCAATGTAGCAAAGCTATTGACATAATGAGGCAGAAGCTACATGGCCTTCACCAGGA
TGTTTTTAATAGATTAAGAGATGACCAAGGTAGGCCAGTCAATCCAAGCCAATATGCACTTCAGTACAGC
CATGATGGAACTGTTTTGATTGTACCCAAAGAACTCATCTCATCAGGCCACAAAAAGGAAGCCCCAAAGG
GAAAGAGAGAGAGAAGAAAC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209844 protein sequence
Red=Cloning site Green=Tags(s)

MKLPKPWWAGGGLLQLTILLSLVGLRVDLDLFLPPPAAALWEELLPLCPTRPASASNPFSASEGWERTPL
LPAKGRLLHEVRALGVPFIPRTRVDAWLVHSVATGNADGAHGLLGTAASSAVGDGGQSASAGGGDPRAAH
SSPLAAEEEEEKAAEPTAQVPDAGGCGSQENGMLREKSEAVDHSSQQEENEEGVSAQAKSRQQSKTEEHK
MACASAREAEKITEARNESHLHWSDTSFSLEDLFQLLSSQPEHSLEGISVEDIPPFLSSVCESVNSSAQN
INLSQAISHDVNLHEAMLLYPNNTFRRDPSARSSQAQEPFLQLNPHTNPEQATPAMSLPPFDNQMRNLTS
QDLLYDLDSNIFDGINLMSLATGFSPLEVSQLFEEPDSGLSLNSSYNSTSLTNSYRIYDGTVGYNSDLQS
LCHDLGAVGGCYPEPHKHCHMDHRTIAGFHVSLECQQVFHDHTYHLQSGASEPTSESFAWSEKSQKTSGC
LDNPDRNLSRDEQRAKALHIPFSVDEIVRMPVDSFNSMLSRYYLTDLQVSLIRDIRRRGKNKVAAQNCRK
RKLDIILNLEDDICNLQAKKEALKNEQTQCSKAIDIMRQKLHGLHQDVFNRLRDDQGRPVNPSQYALQYS
HDGTVLIVPKELISSGHKKEAPKGKRERRN

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_010903
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010903.2
RefSeq Size 2544 bp
RefSeq ORF 1983 bp
Locus ID 18025
UniProt ID Q9WTM4
Cytogenetics 6 24.84 cM
MW 72.7 kDa
Summary Activates erythroid-specific, globin gene expression.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010903" in other vectors (4)
SKU Description Size Price
MC200880 Nfe2l3 (untagged) - Mouse nuclear factor, erythroid derived 2, like 3 (Nfe2l3), (10ug) 10 ug
$615.00
MG209844 Nfe2l3 (tGFP-tagged) - Mouse nuclear factor, erythroid derived 2, like 3 (Nfe2l3) 10 ug
$815.00
MR209844L3 Lenti ORF clone of Nfe2l3 (Myc-DDK-tagged) - Mouse nuclear factor, erythroid derived 2, like 3 (Nfe2l3) 10 ug
$915.00
MR209844L4 Lenti ORF clone of Nfe2l3 (mGFP-tagged) - Mouse nuclear factor, erythroid derived 2, like 3 (Nfe2l3) 10 ug
$915.00

Plasmid Map for MR209844

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209844
Circular map for MR209844

file-alt Citations

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