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Flrt2 (NM_201518) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209836
Flrt2 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 2 (Flrt2)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $614.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Flrt2
Synonyms KIAA0405
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209836 representing NM_201518
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCTACAGACTACAAAGTGGCCCGGCCGTGGGGCTTTCATCCTCAAATTTTGGCTCATCATTTCCC
TGGGACTCTACTTACAAGTGTCAAAACTCCTGGCCTGCCCTAGTGTATGCCGCTGTGACAGGAACTTTGT
CTACTGTAACGAGAGAAGCTTGACCTCAGTGCCTCTTGGGATCCCGGAGGGCGTAACCGTACTCTACCTC
CACAACAACCAAATTAATAATGCTGGCTTTCCTGCAGAGCTGCACAATGTCCAGTCAGTGCACACTGTCT
ACCTTTATGGCAACCAGTTGGATGAGTTCCCCATGAACCTTCCCAAGAATGTCAGAGTTCTCCATCTGCA
GGAAAACAACATTCAGACCATCTCGCGGGCTGCTCTCGCTCAGCTCCTCAAGCTGGAAGAACTCCACCTG
GACGACAACTCCATATCTACAGTGGGAGTAGAAGACGGAGCGTTCCGGGAGGCGATTAGCCTCAAACTGT
TGTTTTTATCGAAGAATCACCTGAGTAGCGTGCCTGTTGGGCTTCCTGTAGACTTGCAAGAGCTGAGAGT
GGATGAAAACCGAATTGCCGTCATATCAGACATGGCCTTTCAGAACCTCACAAGCTTGGAGCGCCTGATC
GTGGATGGGAATCTTCTGACCAACAAGGGCATTGCTGAGGGTACCTTCAGTCATCTCACCAAGCTCAAGG
AATTTTCTATAGTCCGGAACTCGCTCTCCCACCCACCTCCTGATCTCCCAGGTACGCATCTGATCAGGCT
CTACTTGCAGGATAACCAGATAAACCATATCCCATTGACAGCCTTCGCAAACCTCCGTAAGCTGGAAAGG
CTAGATATATCCAACAACCAGCTACGAATGTTAACTCAAGGAGTCTTCGATCATCTCTCCAACCTGAAAC
AACTCACTGCGCGGAATAACCCTTGGTTTTGTGACTGCAGCATTAAATGGGTCACAGAATGGCTCAAGTA
TATCCCTTCTTCTCTCAACGTGCGTGGTTTCATGTGCCAAGGTCCTGAGCAAGTCCGCGGGATGGCTGTC
AGGGAGTTGAATATGAATCTTTTGTCTTGTCCCACCACGACTCCTGGCCTACCTGTCTTTACCCCAGCTC
CAAGTACCGTTTCTCCAACAACTCAGTCTCCTACTCTCTCTGTTCCAAGCCCCAGCAGAGGCTCTGTGCC
TCCAGCTCCTACCCCATCGAAACTTCCCACCATCCCTGATTGGGATGGCAGAGAAAGAGTGACCCCACCT
ATTTCTGAAAGGATCCAACTTTCCATCCACTTTGTTAATGATACTTCGATTCAAGTCAGCTGGCTGTCTC
TCTTTACTGTGATGGCCTACAAACTGACATGGGTGAAAATGGGCCACAGTCTCGTAGGGGGCATCGTTCA
GGAACGAATTGTCAGTGGTGAGAAGCAACACCTGAGCTTGGTTAATTTAGAGCCCAGATCCACGTATAGG
ATTTGTTTAGTGCCGCTGGATGCGTTCAACTACCGCACTGTGGAAGATACCATCTGTTCGGAGGCTACCA
CCCATGCCTCTTATTTGAACAACGGCAGCAACACTGCTTCTAGCCATGAGCAGACGACTTCCCACAGTAT
GGGCTCCCCTTTTCTGCTCGCAGGCTTGATTGGGGGCGCAGTGATTTTTGTGCTCGTTGTCTTGCTCAGC
GTCTTTTGCTGGCACATGCACAAAAAGGGACGCTACACCTCCCAGAAGTGGAAATACAACCGGGGCCGAC
GGAAAGACGACTATTGTGAAGCGGGTACCAAAAAAGACAACTCCATCTTGGAGATGACAGAAACAAGTTT
TCAGATTGTCTCCTTAAATAACGATCAGCTCCTTAAAGGAGATTTCAGACTGCAGCCCATTTATACCCCA
AATGGGGGCATTAATTACACAGACTGCCACATCCCCAACAACATGAGATACTGCAACAGCAGTGTGCCAG
ATCTAGAGCATTGCCATACG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209836 representing NM_201518
Red=Cloning site Green=Tags(s)

MGLQTTKWPGRGAFILKFWLIISLGLYLQVSKLLACPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYL
HNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHL
DDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLI
VDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFANLRKLER
LDISNNQLRMLTQGVFDHLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAV
RELNMNLLSCPTTTPGLPVFTPAPSTVSPTTQSPTLSVPSPSRGSVPPAPTPSKLPTIPDWDGRERVTPP
ISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYR
ICLVPLDAFNYRTVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGSPFLLAGLIGGAVIFVLVVLLS
VFCWHMHKKGRYTSQKWKYNRGRRKDDYCEAGTKKDNSILEMTETSFQIVSLNNDQLLKGDFRLQPIYTP
NGGINYTDCHIPNNMRYCNSSVPDLEHCHT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201518
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201518.4, NP_958926.1
RefSeq Size 7099 bp
RefSeq ORF 1983 bp
Locus ID 399558
UniProt ID Q8BLU0
Cytogenetics 12 E
MW 74.4 kDa
Summary Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:21350012, PubMed:25728924 PubMed:25374360). May play a role in the migration of cortical neurons during brain development via its interaction with UNC5D (PubMed:21673655). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5D, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25728924). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal organization of the cardiac basement membrane during embryogenesis, and for normal embryonic epicardium and heart morphogenesis (PubMed:21350012).UniProtKB/Swiss-Prot Function
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"NM_201518" in other vectors (3)
SKU Description Size Price
MC220214 Flrt2 (untagged) - Mouse fibronectin leucine rich transmembrane protein 2 (Flrt2), (10ug) 10 ug
$677.00
MR209836L3 Lenti ORF clone of Flrt2 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 2 (Flrt2) 10 ug
$914.00
MR209836L4 Lenti ORF clone of Flrt2 (mGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 2 (Flrt2) 10 ug
$914.00

Plasmid Map for MR209836

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209836
Circular map for MR209836

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