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Eef1d (NM_029663) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209833
Eef1d (Myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $615.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eef1d
Synonyms EF-1-delta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209833 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGTGGGAAGGCCTCTTGTGCACTGGAGACCGTCTGGGAGGACAGGCACAAGTATGAGGGAGCCG
AGCGGCGCTTCCACGAGCAGGAGGCCACACAAGTCGTCGCCGCCGCCGCCACTGCCGCCGCCACTGCCTC
CGCCTCTGTCCAGCAGCTCCTGGACGAAGTGCCAGCTGTGAACGGGCCCAGCCAGGAGGACACAGAGGAC
ACTGAGGAGGCAGAGGCCCCAAACACCAGCAGCAGGAGTGATCCCGGGAAGAGCCACGAGTGCAAGAAGC
CGATACAGAAAAAGAGGAAGCGCTCCCCGAAAAGTTGGCTGGGCCAGGCAGACCTGGCCCTTGTGGGCCT
CTCGGCTGACCACGTATGGTTAGACAAGCCACTCTTCGACCAGGCAGAAAGCTCCTACCGTCAGAGGCTG
GCAGATGTAGCTGCCCAGGCAGCCCAGCCGCCTGCTCTGGCCCCTCGGGGGCCCTGTACACATGGAAGCC
ATGTGGCATGCCACCATGTAACCTGGGGGATCTGGGTCAACAAGTCTTGCTTTGATCAAGCCGAGCGGGC
TTTTGTGGAGTGGTCTCAGTCCTTGCTGCTGGCTGCAGAGGGGAGCCATAGGCAGGGGACTCCTGACACA
GGCCAGCAGGCTGTCACTCCTGACCTGGCCTTGGCCTGCCAGCCTTGCCCACCAGCCAATGGCCAGCCTC
CTCTGGGCAGCCTGCAGGCACTGGTGCGGGAGGTATGGCTGGAGAAGCCCCGGTACGATGCCGCTGAGAG
GGGCTTCTATGAGGCCCTGTTTGATGGTCATCCCCCAGGGAAAGTGCGCCTGCAGGAGCGAGCCAGTCAG
GCAGAGGGTACCCGGAGGGGCCGCAGAGACCATCGGAGCTGCAATAACGTAGGAAACAAGAGAGCTGGGT
CAAAACGGGCCAACGGGGAGGCTCCTCCTGCTTTTCCCTACTGGTACTTCCTACACAAGGACGCAGAGGC
CCCCTGGCTCAGCAAGCCTACCTACGACAGCGCTGAGTGCCGCCACCATGCCGTTGAGGCCCTGCGCATA
GCCTGGCGCCTAGAAGCTGCCTCCCTGGCTCACCGACCCACACCCCGTTCTGGCCCATCCATGTCCAGCC
TGAGACCCAACAGAAAAATGGCTACAAACTTTCTAGCGCATGAGAAGATCTGGTTTGACAAGTTTAAATA
TGATGATGCAGAAAGGAGATTCTATGAGCAGATGAACGGGCCTGTGACCTCCGGCTCCCGCCAGGAGAAT
GGTGCCAGCGTGATCCTCCGAGACATTGCAAGAGCCAGAGAGAACATCCAGAAATCCTTGGCTGGAAGCT
CAGGCCCTGGAGCCTCCAGTGGACCTGGTGGAGACCACAGTGAGCTCATTGTGAGGATTACCAGTCTGGA
AGTGGAGAACCAGAACCTTCGAGGCGTGGTGCAAGATTTGCAGCAGGCCATTTCCAAGTTGGAGGCCCGG
CTGAGCTCTCTAGAGAAGAGTTCACCTACTCCCCGAGCCACGGCCCCACAGACCCAACATGTCTCTCCTA
TGCGTCAAGTGGAGCCCCCAACCAAGAAAGGAGCCACACCAGCAGAGGACGATGAGGACAAGGACATTGA
CCTGTTCGGCAGTGACGAGGAGGAAGAAGATAAGGAGGCTGCCCGACTACGGGAGGAGAGGCTACGCCAG
TACGCAGAGAAGAAGGCCAAGAAGCCCACACTGGTGGCCAAATCCTCCATCCTTTTGGATGTTAAACCTT
GGGATGATGAGACTGACATGGCCCAGCTAGAGACTTGTGTGCGTTCCATCCAATTGGACGGGCTGGTTTG
GGGGGCCTCCAAGCTTGTGCCTGTTGGCTATGGCATCCGGAAGCTGCAGATCCAGTGTGTGGTGGAGGAT
GACAAAGTGGGCACCGACTTGCTCGAGGAGGAGATCACCAAATTTGAGGAGCATGTGCAGAGTGTCGACA
TCGCAGCTTTCAACAAGATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209833 protein sequence
Red=Cloning site Green=Tags(s)

MRSGKASCALETVWEDRHKYEGAERRFHEQEATQVVAAAATAAATASASVQQLLDEVPAVNGPSQEDTED
TEEAEAPNTSSRSDPGKSHECKKPIQKKRKRSPKSWLGQADLALVGLSADHVWLDKPLFDQAESSYRQRL
ADVAAQAAQPPALAPRGPCTHGSHVACHHVTWGIWVNKSCFDQAERAFVEWSQSLLLAAEGSHRQGTPDT
GQQAVTPDLALACQPCPPANGQPPLGSLQALVREVWLEKPRYDAAERGFYEALFDGHPPGKVRLQERASQ
AEGTRRGRRDHRSCNNVGNKRAGSKRANGEAPPAFPYWYFLHKDAEAPWLSKPTYDSAECRHHAVEALRI
AWRLEAASLAHRPTPRSGPSMSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVTSGSRQEN
GASVILRDIARARENIQKSLAGSSGPGASSGPGGDHSELIVRITSLEVENQNLRGVVQDLQQAISKLEAR
LSSLEKSSPTPRATAPQTQHVSPMRQVEPPTKKGATPAEDDEDKDIDLFGSDEEEEDKEAARLREERLRQ
YAEKKAKKPTLVAKSSILLDVKPWDDETDMAQLETCVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVED
DKVGTDLLEEEITKFEEHVQSVDIAAFNKI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029663
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029663.2
RefSeq Size 2855 bp
RefSeq ORF 1983 bp
Locus ID 66656
UniProt ID P57776
Cytogenetics 15 D3
MW 72.9 kDa
Summary Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.UniProtKB/Swiss-Prot Function
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Documents
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"NM_029663" in other vectors (3)
SKU Description Size Price
MG209833 Eef1d (tGFP-tagged) - Mouse eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), transcript variant 10 ug
$815.00
MR209833L3 Lenti ORF clone of Eef1d (Myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), transcript variant 1 10 ug
$915.00
MR209833L4 Lenti ORF clone of Eef1d (mGFP-tagged) - Mouse eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), transcript variant 1 10 ug
$915.00

Plasmid Map for MR209833

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209833
Circular map for MR209833

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