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Hal (NM_010401) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209814
Hal (Myc-DDK-tagged) - Mouse histidine ammonia lyase (Hal)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $612.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "Hal or NM_010401"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hal
Synonyms his; hist; histidase; Hs; Hsd
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209814 representing NM_010401
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTAGGTACACAGTGCACGTGCGTGGGGAATGGCTGGCGGTGCCCTGTCAGGATGGGAAGCTCACTG
TTGGCTGGCTGGGCCGTGAGGCTGTGCGACGCTACATGAAGAACAAACCGGACAATGGTGGCTTCACTTC
GGTGGATGAAGTGCAATTCCTTGTGCATCGGTGCAAGGGCCTGGGCCTGCTGGACAATGAGGACGAGCTG
GAGGTGGCCTTAGAGGACAATGAGTTCGTAGAAGTGGTGATTGAAGGTGATGTGATGTCTCCTGATTTCA
TTCCGTCGCAGCCAGAAGGAGTTTTCCTATACAGCAAATACCGGGAGCCTGAAAAATACATTGCCCTAGA
TGGGGACAGTCTGAGCACAGAGGATCTGGTCAACCTGGGCAAGGGACGTTACAAGATAAAGCTCACTTCA
ATTGCTGAGAAGAAAGTGCAGCAGTCCCGGGAGGTCATCGACAGCATCATCAAAGAAAGAACAGTTGTTT
ATGGCATCACCACAGGTTTTGGAAAATTTGCTAGAACTGTAATCCCTGCCAATAAGCTACAGGAGCTTCA
AGTCAACTTAGTCCGTTCCCATTCTTCAGGTGTTGGGAAACCACTCAGTCCTGAGAGGTGTCGGATGCTC
TTGGCTTTGAGGATCAACGTCTTAGCCAAAGGTTACAGTGGGATTTCCTTGGAGACGCTGAAACAAGTTA
TTGAAGCATTTAATGCCTCCTGCCTGTCCTATGTTCCAGAGAAAGGAACTGTGGGTGCCAGCGGAGACCT
TGCCCCACTCTCTCATCTTGCTCTGGGGCTCATCGGAGAAGGAAAGATGTGGTCCCCGAAGAGTGGCTGG
GCAGATGCTAAATATGTTCTAGAAGCCCATGGACTGAAACCAATTGTCTTGAAACCAAAAGAAGGCCTGG
CACTCATCAATGGGACACAGATGATCACTTCCCTGGGCTGTGAAGCCCTGGAGCGAGCCAGCGCCATTGC
CCGGCAAGCTGATATTGTGGCTGCCTTGACCCTGGAGGTGCTGAAGGGCACCACCAAAGCCTTCGATACC
GACATCCATGCTGTCCGACCTCACCGTGGGCAAATTGAAGTTGCTTTCCGATTCCGGTCCCTCCTGGACT
CGGATCACCACCCATCAGAAATCGCAGAAAGCCACAGGTTCTGCGATCGTGTTCAGGACGCGTACACCTT
ACGCTGCTGTCCACAGGTCCACGGTGTGGTGAACGACACAATAGCCTTTGTGAAGGACATCATCACTACA
GAACTGAACAGCGCCACAGACAATCCTATGGTCTTTGCCAGTCGAGGGGAGACAATTTCAGGAGGAAACT
TCCATGGTGAATACCCAGCCAAAGCCCTGGACTATTTGGCCATTGGTGTCCATGAACTTGCGGCAATTAG
TGAAAGAAGAATCGAAAGGCTGTGTAATCCTTCCCTCAGCGAGCTGCCTGCCTTCCTGGTGGCTGAAGGA
GGTCTCAATTCTGGGTTCATGATAGCCCACTGCACCGCAGCAGCCCTGGTGTCTGAGAGCAAGGCTCTGT
GCCACCCTTCATCTGTGGACTCACTCTCGACCAGTGCTGCTACAGAAGACCACGTCTCCATGGGAGGATG
GGCAGCCAGGAAAGCCCTCAGGGTCGTCGAGCACGTGGAACAAGTGCTAGCCATTGAGCTTCTCGCGGCC
TGCCAGGGTATAGAGTTTCTGCGCCCCCTGAAAACAACCACTCCGCTGGAGAAGGTGTACGACCTGGTGC
GCTCCGTAGTAAGGCCCTGGATAAAAGATCGCTTCATGGCCCCGGACATTGAGGCAGCACACAGGTTGCT
TCTTGACCAAAAGGTTTGGGAGGTTGCCGCACCGTACATCGAGAAATACAGAATGGAGCACATTCCGGAA
TCCAGACCGCTTTCTCCAACAGCGTTTTCACTAGAATCTCTCCGAAAGAACTCAGCCACAATCCCAGAGT
CTGACGATCTT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209814 representing NM_010401
Red=Cloning site Green=Tags(s)

MPRYTVHVRGEWLAVPCQDGKLTVGWLGREAVRRYMKNKPDNGGFTSVDEVQFLVHRCKGLGLLDNEDEL
EVALEDNEFVEVVIEGDVMSPDFIPSQPEGVFLYSKYREPEKYIALDGDSLSTEDLVNLGKGRYKIKLTS
IAEKKVQQSREVIDSIIKERTVVYGITTGFGKFARTVIPANKLQELQVNLVRSHSSGVGKPLSPERCRML
LALRINVLAKGYSGISLETLKQVIEAFNASCLSYVPEKGTVGASGDLAPLSHLALGLIGEGKMWSPKSGW
ADAKYVLEAHGLKPIVLKPKEGLALINGTQMITSLGCEALERASAIARQADIVAALTLEVLKGTTKAFDT
DIHAVRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTLRCCPQVHGVVNDTIAFVKDIITT
ELNSATDNPMVFASRGETISGGNFHGEYPAKALDYLAIGVHELAAISERRIERLCNPSLSELPAFLVAEG
GLNSGFMIAHCTAAALVSESKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVVEHVEQVLAIELLAA
CQGIEFLRPLKTTTPLEKVYDLVRSVVRPWIKDRFMAPDIEAAHRLLLDQKVWEVAAPYIEKYRMEHIPE
SRPLSPTAFSLESLRKNSATIPESDDL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_010401
ORF Size 1971 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010401.4
RefSeq Size 2758 bp
RefSeq ORF 1974 bp
Locus ID 15109
UniProt ID P35492
Cytogenetics 10 48.49 cM
MW 72.3 kDa
Summary This gene encodes a member of the histidase protein family. The encoded protein is a cytosolic enzyme which catalyzes the first reaction in histidine catabolism. Defects in this protein cause histidinemia, which is characterized by increased histidine in blood, urine, and cerebrospinal fluid. provided by RefSeq, Dec 2015
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"NM_010401" in other vectors (4)
SKU Description Size Price
MC204658 Hal (untagged) - Mouse histidine ammonia lyase (Hal), (10ug) 10 ug
$612.00
MG209814 Hal (tGFP-tagged) - Mouse histidine ammonia lyase (Hal) 10 ug
$812.00
MR209814L3 Lenti ORF clone of Hal (Myc-DDK-tagged) - Mouse histidine ammonia lyase (Hal) 10 ug
$912.00
MR209814L4 Lenti ORF clone of Hal (mGFP-tagged) - Mouse histidine ammonia lyase (Hal) 10 ug
$912.00

Plasmid Map for MR209814

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209814
Circular map for MR209814

file-alt Citations

Moderate UV Exposure Enhances Learning and Memory by Promoting a Novel Glutamate Biosynthetic Pathway in the Brain
Zhu, Wang, Yao et al
Cell (2018)
AI Summary: OriGene products, specifically urocanase, IPPAnase, and FTCD plasmids, were used in this study to manipulate and investigate the enzymes involved in the novel glutamate biosynthetic pathway activated by UV exposure in the brain.
Snippet: rocanase plasmids OriGene N/A IPPAnase plasmids OriGene N/A FTCD plasmids OriGene N/A pRSV-Rev Dull et al., 1998 Addgene Cat# 12253 pMDLg/pRRE Dull et al., 1998 Addgene Cat# 12251 pMD2.G Gift from Didier Trono (unpublished data) Addgene Cat# 12259
Showing 1-1 of 1 papers.
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