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Vps53 (BC034371) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209806
Vps53 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $610.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vps53
Synonyms RP23-147P4.2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209806 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAGGAGGAGGAACTGGAGTTTGTGGAGGAGCTGGAGGCTGTGCTGCAGCTCACGCCAGAGGTGC
AACTCGCCATCGAGCAGGTATTCCCAAGCCAGGATCCTCTTGATCAAGCTGACTTCAATGCCGTTGAGTA
TATCAATACTCTGTTCCCAACAGAGCAATCTCTGGCAAACATTGACGATGTTGTGAACAAAATTAGACTG
AAAATAAGGAGACTGGATGACAATATCCGGACTGTCGTAAGAGGTCAGACAAATGTGGGGCAGGATGGAA
GGCAAGCACTTGAAGAGGCCCAGAAAGCCATTCAGCAACTCTTTGGCAAAATCAAGGATATCAAAGACAA
AGCAGAAAAGTCAGAACAAATGGTGAAAGAGATCACCCGAGATATTAAGCAGTTAGATCACGCCAAGCGC
CACCTGACCACCTCAATCACGACCCTGAACCACCTGCACATGCTGGCAGGCGGGGTGGACTCCCTGGAAG
CCATGACCAGGCGCAGACAGTATGGAGAAGTTGCTAATCTCCTTCAGGGTGTAATGAATGTTCTGGAGCA
CTTTCACAAATACATGGGAATCCCACAGATCCGACAGCTTTCTGAAAGGGTGAAGGCTGCACAGACAGAG
TTAGGACAGCAAATCCTGGCTGACTTTGAAGAAGCGTTTCCTTCGCAGGGTACCAAGCCGAAGAAGCCCA
AGGCCCCGGACAACCCGTTCCACGGCATCGTTTCCAAGTGTTTTGAGCCTCATCTCTACGTGTACATTGA
GTCCCAGGACAAAAACCTCAGCGAGCTGATAGACCGGTTTGTGGCAGATTTCAAAGCCCAGGGCCCACCC
AAGCCCAACACTGACGAAGGGGGTGCTGTGCTCCCCAGCTGCGCTGACCTCTTTGTCTACTACAAGAAGT
GCATGGTGCAGTGCTCACAGCTCAGCACAGGCGAGCCCATGATCGCCCTGACTACCATCTTCCAGAAGTA
CCTCCGAGAATACGCCTGGAAAATCCTCTCTGGCAACCTGCCTAAAACCAGCAGCAGCAGTGGGGGGCTG
ACCATCAGCAGCCTCCTCAAAGAGAAGGAGGGCTCAGAAGTGGCCAGGTTCACTCTGGAGGAGCTGTGTC
TCATCTGCAGCATCCTGAGCACAGCCGAGTATTGTCTGGCTACCACCCAGCAGCTAGAAGAAAAACTCAA
AGAAAAAGTTGATGTGAGCCTGACTGAACGAATTAATCTCACAGGAGAAATGGACACATTCAGCACTGTC
ATCTCCAGCAGCATCCAGCTGTTGGTTCAAGATCTGGATGCAGCCTGTGACCCTGCCCTGATTGCCATGA
GCAAGATGCCTTGGCAGAACGTGGAGCATGTTGGTGACCAGAGCCCTTATGTCACTTCTGTTATTCTTCA
CATTAAGCAAAACGTCCCCATCATCCGTGACAACCTGGCTTCCACACGGAAATACTTCACACAGTTCTGC
ATTAAATTTGCAAATTCGTTCATTCCCAAATTCATCACCCACCTCTTCAAGTGTAAGCCGATCAGCATGG
TGGGAGCAGAGCAGCTTCTGCTGGACACCCACTCCCTGAAGATGGTCCTGCTCGACCTTCCGTCCATTGG
CTCCCAGGTGGTGAGGAAGGCCCCTGCCAGTTACACTAAGATTGTCGTGAAAGGCATGACCCGGGCTGAA
ATGATCCTTAAGGTGGTGATGGCCCCTCATGAACCGTTGGTGGTATTTGTTGACAACTACATCAAACTCC
TTACGGACTGCAACTCAGAAACCTTCCAGAAAATACTGGATATGAAGGGGCTGAAGAGGAGTGAGCAGAG
CAGCATGCTGGAACTTTTGCGGCAGAGGCTCCCTGCCCCGCCCTCGGGGACAGAAGGTTCCAGCACCCTG
TCGCTGATTGCCCCAACACCGGAGCAGGAGTCATCCCGAATCCGCAAACTGGAGAAACTGATTAAAAAGA
GACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209806 protein sequence
Red=Cloning site Green=Tags(s)

MMEEEELEFVEELEAVLQLTPEVQLAIEQVFPSQDPLDQADFNAVEYINTLFPTEQSLANIDDVVNKIRL
KIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKR
HLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVMNVLEHFHKYMGIPQIRQLSERVKAAQTE
LGQQILADFEEAFPSQGTKPKKPKAPDNPFHGIVSKCFEPHLYVYIESQDKNLSELIDRFVADFKAQGPP
KPNTDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTSSSSGGL
TISSLLKEKEGSEVARFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDVSLTERINLTGEMDTFSTV
ISSSIQLLVQDLDAACDPALIAMSKMPWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFC
IKFANSFIPKFITHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAE
MILKVVMAPHEPLVVFVDNYIKLLTDCNSETFQKILDMKGLKRSEQSSMLELLRQRLPAPPSGTEGSSTL
SLIAPTPEQESSRIRKLEKLIKKRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC034371
ORF Size 1965 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC034371, AAH34371
RefSeq Size 2225 bp
RefSeq ORF 1967 bp
Locus ID 68299
Cytogenetics 11 B5
MW 73.7 kDa
Summary Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.UniProtKB/Swiss-Prot Function
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"BC034371" in other vectors (4)
SKU Description Size Price
MC218399 Vps53 (untagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008), (10ug) 10 ug
$672.00
MG209806 Vps53 (tGFP-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008) 10 ug
$810.00
MR209806L3 Lenti ORF clone of Vps53 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008) 10 ug
$910.00
MR209806L4 Lenti ORF clone of Vps53 (mGFP-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008) 10 ug
$910.00

Plasmid Map for MR209806

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209806
Circular map for MR209806

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