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Mx2 (NM_013606) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209802
Mx2 (Myc-DDK-tagged) - Mouse myxovirus (influenza virus) resistance 2 (Mx2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $611.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mx2
Synonyms AI528743; Mx-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209802 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCTTAGCACAGAAGAAAACACAGGCGTTGATTCAGTCAACTTACCCAGTGGAGAGACGGGCCTAG
GAGAGAAAGACCAGGAGTCTGTGAACAATCTCTGCAGTCAGTATGAGGAGAAGGTGCGGCCCTGCATTGA
CCTCATCGACTCCCTGCGGGCTCTGGGTGTGGAGCAGGACCTGGCCCTGCCTGCCATCGCTGTCATTGGG
GACCAGAGTTCAGGGAAGAGCTCTGTGCTGGAAGCACTGTCTGGAGTGGCCCTCCCCAGAGGCAGTGGTA
TTGTCACCAGATGCCCTCTGGTGCTGAAATTGAGGAAGCTGAATGAGGGAGAGGAGTGGAGAGGCAAAGT
CTCCTATGATGACATCGAAGTGGAGCTCTCTGATCCTTCAGAGGTGGAAGAGGCCATCAACAAGGGTCAG
AACTTCATTGCTGGGGTAGGTCTGGGGATCAGTGATAAGCTCATTAGCCTGGATGTCAGCTCCCCAAATG
TCCCAGACCTGACTCTCATTGACCTGCCTGGAATTACCAGGGTGGCTGTAGGCAACCAGCCTGCAGACAT
AGGACGCCAGATCAAGAGACTCATCAAGACATACATCCAAAAACAGGAGACCATCAACCTGGTGGTGGTC
CCCAGCAATGTGGACATTGCCACCACAGAGGCTCTGAGCATGGCTCAGGAGGTGGACCCTGAAGGGGATA
GGACCATAGGGATCTTGACCAAGCCCGATCTGGTGGACAGAGGTACTGAAGACAAGGTCGTGGATGTGGT
GCGGAACCTGGTGTACCATCTGAAGAAGGGCTACATGATAGTCAAGTGCAGAGGCCAGCAGGACATCCAA
GAGCAGCTGAGCCTGACTGAGGCTCTTCAGAATGAGCAAATCTTCTTCAAGGAACACCCTCATTTCAGAG
TTCTTCTGGAGGATGGGAAGGCCACAGTGCCCTGCCTGGCAGAGAGACTGACCGCAGAGCTCATCTCACA
CATCTGTAAATCTCTCCCTCTGCTAGAAAATCAAATAAAGGAAAGTCACCAGAGTGCAAGTGAGGAGCTG
CAGAAGTACGGTATGGACATACCAGAAGATGACAGTGAAAAAACTTTCTTCCTGATCGAAAAAATCAATG
CTTTTAACCAGGACATCACTGCCCTAGTACAAGGGGAAGAGAATGTGGCAGAGGGAGAATGTCGCCTATT
CACCAGGCTCCGAAAAGAGTTCTTGTCCTGGAGTAAGGAGATCGAAAAGAATTTCGCAAAAGGTTATGCT
GTTTTATATAATGAAGTCTGGGCATTTGAAAAGCAGTATCGTGGCCGAGAATTGCCAGGGTTTGTGAATT
ACAAAACATTTGAGAACATTATAAGAAGACAAATCAAAACCCTGGAAGAACCAGCTATAGAAATGCTGCA
CACGGTCACTGAAATTGTACGAGCTGCCTTTACAAGTGTTTCTGAGAAAAATTTTTCTGAGTTTTATAAC
CTCCACAGAACTACCAAGTCCAAACTTGAAGACATCAGATTAGAACAAGAAAAGGAAGCAGAGATGTCGA
TCCGACTCCACTTCAAGATGGAACAAATTATCTACTGCCAGGACCAGATTTACAGAGGAGCCTTGCAGAA
GGTCAGAGAGGAGGAAGCTGAGGAGGAGAAGAAGACAAAGCATGGCACTTCCAGTTCCTCTCAGTCCCAA
GATTTACAGACCTCATCCATGGCTGAGATCTTCCAGCATCTGAATGCCTACCGCCAGGAGGCTCACAACC
GCATCTCCAGCCACGTTCCCTTGATCATCCAGTATTTCATCCTGAAAATGTTTGCTGAGCGGCTGCAGAA
GGGCATGCTCCAGCTCCTGCAGGACAAGGATTCCTGCAGCTGGCTCCTGAAGGAGCAGAGTGACACAAGC
GAGAAGAGGAAGTTCCTGAAGGAGAGGCTGGCAAGGCTGGCCCAGGCTCGGCGCCGGCTAGCCAAATTCC
CTGGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209802 protein sequence
Red=Cloning site Green=Tags(s)

MVLSTEENTGVDSVNLPSGETGLGEKDQESVNNLCSQYEEKVRPCIDLIDSLRALGVEQDLALPAIAVIG
DQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLRKLNEGEEWRGKVSYDDIEVELSDPSEVEEAINKGQ
NFIAGVGLGISDKLISLDVSSPNVPDLTLIDLPGITRVAVGNQPADIGRQIKRLIKTYIQKQETINLVVV
PSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDRGTEDKVVDVVRNLVYHLKKGYMIVKCRGQQDIQ
EQLSLTEALQNEQIFFKEHPHFRVLLEDGKATVPCLAERLTAELISHICKSLPLLENQIKESHQSASEEL
QKYGMDIPEDDSEKTFFLIEKINAFNQDITALVQGEENVAEGECRLFTRLRKEFLSWSKEIEKNFAKGYA
VLYNEVWAFEKQYRGRELPGFVNYKTFENIIRRQIKTLEEPAIEMLHTVTEIVRAAFTSVSEKNFSEFYN
LHRTTKSKLEDIRLEQEKEAEMSIRLHFKMEQIIYCQDQIYRGALQKVREEEAEEEKKTKHGTSSSSQSQ
DLQTSSMAEIFQHLNAYRQEAHNRISSHVPLIIQYFILKMFAERLQKGMLQLLQDKDSCSWLLKEQSDTS
EKRKFLKERLARLAQARRRLAKFPG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013606
ORF Size 1965 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013606.1, NP_038634.1
RefSeq Size 2424 bp
RefSeq ORF 1968 bp
Locus ID 17858
UniProt ID Q9WVP9
Cytogenetics 16 57.51 cM
MW 74.3 kDa
Summary This gene encodes a member of the Mx protein family of large GTPases, and functions in the innate immunity system. Interferon alpha/beta treatment or viral infection induces expression of this protein, which subsequently accumulates in the cytoplasm and inhibits viral replication. It has been shown to confer resistance to vesicular stomatitis virus, a member of the rhabdovirus family, but not to influenza virus. This gene produces a functional protein in some feral mouse strains, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a frameshift that inactivates this gene product. provided by RefSeq, Jul 2008
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"NM_013606" in other vectors (6)
SKU Description Size Price
MC205635 Mx2 (untagged) - Mouse myxovirus (influenza virus) resistance 2 (Mx2), (10ug) 10 ug
$611.00
MG209802 Mx2 (tGFP-tagged) - Mouse myxovirus (influenza virus) resistance 2 (Mx2) 10 ug
$811.00
MR209802L1 Lenti ORF clone of Mx2 (Myc-DDK-tagged) - Mouse myxovirus (influenza virus) resistance 2 (Mx2) 10 ug
$911.00
MR209802L2 Lenti ORF clone of Mx2 (mGFP-tagged) - Mouse myxovirus (influenza virus) resistance 2 (Mx2) 10 ug
$911.00
MR209802L3 Lenti ORF clone of Mx2 (Myc-DDK-tagged) - Mouse myxovirus (influenza virus) resistance 2 (Mx2) 10 ug
$911.00
MR209802L4 Lenti ORF clone of Mx2 (mGFP-tagged) - Mouse myxovirus (influenza virus) resistance 2 (Mx2) 10 ug
$911.00

Plasmid Map for MR209802

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209802
Circular map for MR209802

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