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Aplp1 (NM_007467) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209792
Aplp1 (Myc-DDK-tagged) - Mouse amyloid beta (A4) precursor-like protein 1 (Aplp1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $610.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aplp1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209792 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCCACCAGCCCCGCCGCTCGCGGTCAGGGTCGCCGCTGGCGACCGCCGCCGCTGCCGCTGTTGC
TGCCACTGTCATTGCTGCTTCTGCGCGCGCAGCTCGCCGTCGGGAACCTGGCTGTTGGGAGCCCCAGCGC
GGCCGAGGCTCCGGGGTCGGCTCAAGTGGCTGGACTATGTGGGCGTCTAACCCTTCACCGGGACCTGCGC
ACCGGCCGCTGGGAACCAGACCCACAGCGATCACGACGCTGTCTTCTGGACCCGCAGCGCGTGCTGGAGT
ACTGCAGACAGATGTACCCCGAGCTGCACATAGCACGCGTGGAGCAGGCTGCACAGGCCATCCCGATGGA
GCGCTGGTGTGGGGGTACCCGGAGTGGCAGATGCGCCCACCCCCACCATGAGGTTGTGCCCTTCCATTGC
CTGCCTGGCGAATTCGTGAGTGAAGCCCTGCTAGTGCCCGAAGGCTGTCGGTTCTTGCACCAGGAGCGTA
TGGACCAGTGTGAGAGTTCAACCAGGAGGCATCAGGAGGCTCAGGAGGCCTGCAGCTCTCAGGGCCTCAT
CCTGCATGGCTCTGGCATGCTTTTGCCCTGTGGCTCTGATCGGTTCCGAGGTGTGGAGTATGTATGCTGT
CCACCTCCTGCAACTCCCAACCCATCTGGGATGGCAGCTGGTGACCCCTCTACCCGGTCCTGGCCCCTGG
GGGGCAGAGCAGAGGGAGGTGAGGATGAAGAGGAGGTGGAATCTTTCCCTCAGCCAGTAGACGATTACTT
CGTAGAGCCCCCTCAGGCTGAAGAAGAAGAGGAAGAGGAGGAAGAAAGGGCCCCACCTCCCAGCTCCCAC
ACCCCTGTCATGGTTAGCAGAGTCACTCCCACCCCAAGGCCTACTGATGGTGTGGATGTTTACTTTGGCA
TGCCTGGGGAAATCGGCGAGCATGAGGGTTTCCTGAGGGCCAAGATGGACCTGGAGGAGCGTAGGATGCG
CCAGATTAATGAGGTGATGCGTGAATGGGCCATGGCTGACAGCCAATCTAAGAACCTGCCAAAGGCGGAC
AGGCAGGCCCTGAATGAGCACTTCCAGTCCATTCTGCAGACCCTGGAAGAACAAGTGTCTGGTGAACGGC
AACGCCTGGTGGAGACCCACGCCACCAGAGTCATCGCTCTGATCAACGACCAGCGCCGAGCAGCCCTGGA
AGGTTTCCTGGCAGCCTTACAGGGCGATCCGCCTCAGGCTGAGCGAGTTCTGATGGCCCTGAGGCGCTAC
CTGCGCGCCGAGCAGAAAGAGCAGAGGCACACTCTGAGGCACTACCAGCACGTGGCCGCAGTGGATCCTG
AGAAGGCCCAGCAGATGCGCTTTCAGGTCCAGACCCACCTTCAGGTGATCGAAGAGCGAATGAATCAGAG
CCTGGGGCTGCTCGACCAGAACCCTCACCTGGCTCAGGAGCTGCGGCCACAGATCCAGGAGCTTCTCCTT
GCTGAACACTTGGGTCCCAGTGAACTGGACGCCTCTGTGCCCGGGAGCAGCAGTGAGGACAAAGGTAGCC
TCCAGCCTCCCGAATCCAAGGACGATCCCCCAGTGACCCTTCCAAAAGGGTCCACAGATCAAGAGTCATC
CTCCTCTGGGAGAGAGAAGCTAACTCCACTGGAGCAGTATGAGCAAAAGGTGAATGCATCCGCCCCGAGG
GGGTTTCCCTTCCACTCGTCAGATATCCAGCGGGATGAACTGGCTCCTTCCGGGACTGGAGTGTCCCGAG
AGGCCTTGTCAGGTCTGCTGATCATGGGAGCTGGAGGAGGCTCCCTCATTGTCCTATCCTTGCTGCTTCT
GCGCAAGAAGAAACCCTATGGGACTATCAGCCATGGAGTGGTGGAGGTGGACCCCATGCTGACCCTGGAG
GAGCAGCAGCTCCGGGAACTTCAGAGGCATGGCTATGAGAACCCCACCTACCGCTTCCTGGAAGAACGAC
CT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209792 protein sequence
Red=Cloning site Green=Tags(s)

MGPTSPAARGQGRRWRPPPLPLLLPLSLLLLRAQLAVGNLAVGSPSAAEAPGSAQVAGLCGRLTLHRDLR
TGRWEPDPQRSRRCLLDPQRVLEYCRQMYPELHIARVEQAAQAIPMERWCGGTRSGRCAHPHHEVVPFHC
LPGEFVSEALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCC
PPPATPNPSGMAAGDPSTRSWPLGGRAEGGEDEEEVESFPQPVDDYFVEPPQAEEEEEEEEERAPPPSSH
TPVMVSRVTPTPRPTDGVDVYFGMPGEIGEHEGFLRAKMDLEERRMRQINEVMREWAMADSQSKNLPKAD
RQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQGDPPQAERVLMALRRY
LRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVQTHLQVIEERMNQSLGLLDQNPHLAQELRPQIQELLL
AEHLGPSELDASVPGSSSEDKGSLQPPESKDDPPVTLPKGSTDQESSSSGREKLTPLEQYEQKVNASAPR
GFPFHSSDIQRDELAPSGTGVSREALSGLLIMGAGGGSLIVLSLLLLRKKKPYGTISHGVVEVDPMLTLE
EQQLRELQRHGYENPTYRFLEERP

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_007467
ORF Size 1962 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007467.3
RefSeq Size 2408 bp
RefSeq ORF 1965 bp
Locus ID 11803
UniProt ID Q03157
Cytogenetics 7 18.32 cM
MW 72.8 kDa
Summary May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding. Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.UniProtKB/Swiss-Prot Function
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"NM_007467" in other vectors (4)
SKU Description Size Price
MC204541 Aplp1 (untagged) - Mouse amyloid beta (A4) precursor-like protein 1 (Aplp1), (10ug) 10 ug
$610.00
MG209792 Aplp1 (tGFP-tagged) - Mouse amyloid beta (A4) precursor-like protein 1 (Aplp1) 10 ug
$810.00
MR209792L3 Lenti ORF clone of Aplp1 (Myc-DDK-tagged) - Mouse amyloid beta (A4) precursor-like protein 1 (Aplp1) 10 ug
$910.00
MR209792L4 Lenti ORF clone of Aplp1 (mGFP-tagged) - Mouse amyloid beta (A4) precursor-like protein 1 (Aplp1) 10 ug
$910.00

Plasmid Map for MR209792

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209792
Circular map for MR209792

file-alt Citations

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