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Ssh3 (NM_198113) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209758
Ssh3 (Myc-DDK-tagged) - Mouse slingshot homolog 3 (Drosophila) (Ssh3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $605.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ssh3
Synonyms BC028922; mSSH-3L; SSH-3; SSH-3L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209758 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGGTCACAGTGAGTCGCTCGCCGCCGGCAAGTGGCCACTCCACGCCTGTGGGACCCACGCAGG
ACCGAGTGGTAAGACGCAGAGGCCGGCTCCAGCGCAGGCAGAGTTTTGCGGTGCTCCGAGGGGCTGTCCT
GGGACTTCAGGATGGAGGGGACAGTAATGTTGCATCTGAGGCTGACTCTGAGCCAATGGAGGAACCCTCA
GGTGAGGAACAGCCCACCGAGGACCAGACTGACAAAGGGCAAGGACTCCAGAGTCCCTGGAAACAAGTGC
AAAAGCGGCACCTGCACCTCATGGTGGAGCTACTGAGGCCGCAGGATGATATCCGCCTGGCAGCCCAGCT
GGAGGCAGCCCGACCCCCCAGACTTCGCTACCTGCTGGTAGTATCCACGGGAGAAGAACTGAGTGAGGAG
GCCATCCTGCTTGGGGTGGACTTCCCTGACAGCAGTTCCCACAGCTGCACCCTGGGCCTGGTCTTGCCCC
TCTGGAGTGATACCCAGGTGTACCTGGACGGCGATGGGGGCTTCAGTGTGACATCTGGTGGTCAAAGCCG
AATCTTCAAGCCTGTCTCTATCCAGACCATGTGGGCCACACTGCAGGTATTGCACCAGGCATGTGAGGTA
GCTCTAGGCAGTGGCCTTGTGCCTGGTGGCAGTGCCCTTGCCTGGGCTACTTACTACCAGGAGAAACTCA
ACTCTGACCAGGGTTGCCTCAATGAGTGGATGGCCATGTCTGACCTGGAGTCCTTTCGACCACCTAATGC
TGAGCCTGGACAGGCTTCAGAGCAGGAAAAGATGGAGCAGGCGATCCTGGCTGAATTGTGGCAGGTGTTG
GACACCAGTGACCTGGACAGCGTCACTTCTAAAGAGATCCGTCAGGCCCTGGAGCTGCGTCTGGGATGTC
CTCTCCAGCAGTACCGTGACTTTATTGACAACCAGATGTTGCTGCTCATGGCCCAGCAAGACCGGGCCTC
TCGCATCTTCCCCCACCTCTACTTGGGCTCTGAGTGGAATGCTGCCAACCTGGAGGAACTTCAGAAAAAC
AGAGTAAGTCACATCCTGAACATGGCCCGGGAGATTGACAACTTCTTCCCTGAGCGCTTCACCTATTACA
ATGTACGTGTCTGGGATGAGGAATCGGCACAGCTTCTGCCCCACTGGAAGGAGACACATCGATTCATTGA
GGATGCCAGAGCACAGGGCACTCGGGTGCTAGTCCACTGTAAGATGGGTGTCAGCCGTTCTGCCGCCACG
GTACTAGCCTATGCCATGAAACAGTATGGCTGGGACCTGGAGCAAGCCCTGATCCACGTGCAGGAGCTCC
GGCCCATTGTACGCCCCAACCACGGCTTCCTGCGCCAGCTACGGACCTACCAGGGCATTCTGACTGCCAG
CCGGCAGAGCCACGTCTGGGAGCAGAAAGTGGGTGTGGTCTCCCCAGAGGAGCCCCTGGCGCCTGAAGTT
TCTACACCATTGCCACCTCTTCCACCAGAACCAGGGGGCAGTGGGGAGGTGATGGTAATGGGCCTGGAAG
GAAGCCAGGAAACCCCAAAAGAAGAACTTGGACTGCGGCCCCGCATCAACCTTCGAGGGGTCATGCGCTC
CATCAGTCTCCTGGAGCCCTCAGAGTCAGAGAGTACCCCAGAGGCGGGAGGCCTGCCAGAAGTTTTCTCT
TCAGATGAAGAGCCTCTTCATCCATTCTCTCAGCTTTCAAGAGCCAAAGGTGGCCAGCGGGTCCGCAAAG
GGCCTTGGCCTGCCCTGAAGTCTCGACAGTCTGTGGTTGCCCTTCATAGTGCTGCCCTGGTGGCCAGCAG
GACACGGGCCTTCCAAGAACAGGGGCAAGGGCAAGAGCAGAGTGAGCCTGGTATGTCTTCTACCCCTAGG
CTCCGGAAGGTGATGAGGCAGGCCAGTGTAGATGACAGCAGGGAGGAGGACAAGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209758 protein sequence
Red=Cloning site Green=Tags(s)

MALVTVSRSPPASGHSTPVGPTQDRVVRRRGRLQRRQSFAVLRGAVLGLQDGGDSNVASEADSEPMEEPS
GEEQPTEDQTDKGQGLQSPWKQVQKRHLHLMVELLRPQDDIRLAAQLEAARPPRLRYLLVVSTGEELSEE
AILLGVDFPDSSSHSCTLGLVLPLWSDTQVYLDGDGGFSVTSGGQSRIFKPVSIQTMWATLQVLHQACEV
ALGSGLVPGGSALAWATYYQEKLNSDQGCLNEWMAMSDLESFRPPNAEPGQASEQEKMEQAILAELWQVL
DTSDLDSVTSKEIRQALELRLGCPLQQYRDFIDNQMLLLMAQQDRASRIFPHLYLGSEWNAANLEELQKN
RVSHILNMAREIDNFFPERFTYYNVRVWDEESAQLLPHWKETHRFIEDARAQGTRVLVHCKMGVSRSAAT
VLAYAMKQYGWDLEQALIHVQELRPIVRPNHGFLRQLRTYQGILTASRQSHVWEQKVGVVSPEEPLAPEV
STPLPPLPPEPGGSGEVMVMGLEGSQETPKEELGLRPRINLRGVMRSISLLEPSESESTPEAGGLPEVFS
SDEEPLHPFSQLSRAKGGQRVRKGPWPALKSRQSVVALHSAALVASRTRAFQEQGQGQEQSEPGMSSTPR
LRKVMRQASVDDSREEDKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198113
ORF Size 1947 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198113.1
RefSeq Size 2728 bp
RefSeq ORF 1950 bp
Locus ID 245857
UniProt ID Q8K330
Cytogenetics 19 A
MW 72.2 kDa
Summary Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly.UniProtKB/Swiss-Prot Function
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"NM_198113" in other vectors (4)
SKU Description Size Price
MC220132 Ssh3 (untagged) - Mouse slingshot homolog 3 (Drosophila) (Ssh3), (10ug) 10 ug
$665.00
MG209758 Ssh3 (tGFP-tagged) - Mouse slingshot homolog 3 (Drosophila) (Ssh3) 10 ug
$805.00
MR209758L3 Lenti ORF clone of Ssh3 (Myc-DDK-tagged) - Mouse slingshot homolog 3 (Drosophila) (Ssh3) 10 ug
$905.00
MR209758L4 Lenti ORF clone of Ssh3 (mGFP-tagged) - Mouse slingshot homolog 3 (Drosophila) (Ssh3) 10 ug
$905.00

Plasmid Map for MR209758

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209758
Circular map for MR209758

file-alt Citations

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