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Gramd1a (BC018554) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209732
Gramd1a (Myc-DDK-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $602.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gramd1a
Synonyms 1300003M23Rik; AI415002; AW547240; D7Bwg0611e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209732 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGCTGGTACAGTATGCTGTGTCCCACATACAAACAACGCAACGAGGACTTCCGGAAACTGTTCA
GCAAACTTCCTGAAGCAGAGCGCCTCATCGTGGATTACTCCTGCGCCCTGCAGAGGGAGATCCTCCTCCA
GGGCCGCCTCTACCTCTCTGAGAACTGGATCTGTTTCTACAGCAACATCTTCCGCTGGGAGACCACAATT
TCCATCCAGTTGAAGGAAGTGACCTGTCTGAAGAAGGAGAAGACGGCTAAGCTCATCCCCAATGCCATTC
AGATCTGTACTGAGAGTGAGAAGCATTTCTTCACTTCCTTCGGGGCCCGAGACCGCTGCTTCCTCCTCAT
CTTCCGTCTCTGGCAGAATGCGCTGCTTGAAAAGACACTGAGTCCTCGAGAGCTTTGGCACCTGGTCCAT
CAGTGCTATGGCTCGGAGCTGGGCCTCACCAGTGAAGACGAAGACTATGTCTGCCCCTTGCAACTGAATG
GTCTTGGGAGTCCCAAAGAGGTGGGAGACATGATCGCACTCAGCGACATCAGTCCCTCGGGGGCGGCTGA
CCACAGCCAGGAGCCAAGCCCTGTGGGTTCAAGGCGTGGCCGGGTCACCCCAAACCTCTCTCGAGCCAGC
AGTGATGCAGACCATGGGGCCGAGGAGGACAAGGAAGAACAGACGGACAGTCAGCTGGATGCCTCCTCCA
GCCAGACAGTGACCCCAGTAGCAGAACCGCTGAGTTCTGAGCCGACACCACCAGATGGGCCCACTTCCTC
CTTGGGTCCCTTGGATCTGCTGTCTAGAGAGGAACTATTGACAGACACGAGTAACTCGTCCTCATCCACT
GGGGAGGAAGGTGACCTGGCTGCACTGCTGCCTGACCTCTCCGGCCGGCTCCTCATCAACTCCGTCTTCC
ACATGGGTGCTGAGCGTCTGCAGCAGATGCTCTTCTCGGACTCTCCGTTCCTGCAAGGCTTCCTGCAGCA
GCGCAAGTTCACAGATGTGACCTTGAGCCCTTGGAGCAGTGACAGCAAGTGCCACCAGCGCAGAGTCCTG
ACCTACACCATCCCCATTAGCAACCAGCTAGGCCCCAAGAGCGCCTCTGTGGTGGAGACACAGACGCTGT
TCCGGCGCGGCCCACAGGCGGGCGGGTGCGTGGTGGACTCTGAGGTGCTCACCCAGGGCATCCCGTACCA
GGACTACTTCTACACTGCCCACCGATACTGTATCCTGGGTCTGGCCCGGAACAAGGCCCGGCTTCGTGTA
TCCTCAGAGATCCGCTACCGAAAGCAACCGTGGAGCCTCGTAAAGTCGCTCATTGAGAAGAACTCGTGGA
GCGGCATCGAAGATTATTTCCACCACCTGGATCGAGAACTCGCCAAGGCAGAAAAGCTGTCCCTGGAGGA
AGGAGGGAAAGATACACGTGGACTACTGTCAGGCCTGCGGAGGAGGAAGCGGCCCCTGAGCTGGAGGGGG
CACAGAGATGGGCCTCAGCACCCAGACCCAGACCCCTGCACCCAGACCAGCATGCACACCTCAGGTTCCC
TCAGCTCTCGCTTCTCGGAACCATCTGTGGATCAGGGCCCTGGGGCAGGCATCCCCAGCGCCCTGGTTCT
CATCAGCATTGTCCTCATCGTTCTCATCGCCCTCAATGCCCTCCTCTTCTACCGTCTCTGGTCTCTGGAG
AGAACAGCCCATACCTTTGAGTCCTGGCACAGCCTGGCACTGGCCAAGGGCAAGTTCCCCCAGACAGCCA
CGGAGTGGGCGGAGATCTTGGCTCTGCAGAAGCACTTCCACAGCGTTGAGGTGCACAAGTGGAGGCAGAT
CCTTCGAGCATCCGTGGAGCTCCTGGATGAGATGAAGTTCTCCTTGGAGAAGCTACATCAAGGAATTACT
GTCCCGGATCCTCCCCTGGACACTCAGCCACAGCCTGATGACAGTTTTCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209732 protein sequence
Red=Cloning site Green=Tags(s)

MQSWYSMLCPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTI
SIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVH
QCYGSELGLTSEDEDYVCPLQLNGLGSPKEVGDMIALSDISPSGAADHSQEPSPVGSRRGRVTPNLSRAS
SDADHGAEEDKEEQTDSQLDASSSQTVTPVAEPLSSEPTPPDGPTSSLGPLDLLSREELLTDTSNSSSST
GEEGDLAALLPDLSGRLLINSVFHMGAERLQQMLFSDSPFLQGFLQQRKFTDVTLSPWSSDSKCHQRRVL
TYTIPISNQLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRV
SSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLDRELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRG
HRDGPQHPDPDPCTQTSMHTSGSLSSRFSEPSVDQGPGAGIPSALVLISIVLIVLIALNALLFYRLWSLE
RTAHTFESWHSLALAKGKFPQTATEWAEILALQKHFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGIT
VPDPPLDTQPQPDDSFP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC018554
ORF Size 1941 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC018554, AAH18554
RefSeq Size 2513 bp
RefSeq ORF 1943 bp
Locus ID 52857
Cytogenetics 7 19.3 cM
MW 72.8 kDa
Summary Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461). May play a role in tumor progression (PubMed:27585821).UniProtKB/Swiss-Prot Function
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"BC018554" in other vectors (4)
SKU Description Size Price
MC200835 Gramd1a (untagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082), (10ug) 10 ug
$603.00
MG209732 Gramd1a (tGFP-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082) 10 ug
$802.00
MR209732L3 Lenti ORF clone of Gramd1a (Myc-DDK-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082) 10 ug
$902.00
MR209732L4 Lenti ORF clone of Gramd1a (mGFP-tagged) - Mouse GRAM domain containing 1A (cDNA clone MGC:19375 IMAGE:2646082) 10 ug
$902.00

Plasmid Map for MR209732

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209732
Circular map for MR209732

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