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Mcmbp (NM_145955) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209710
Mcmbp (Myc-DDK-tagged) - Mouse MCM (minichromosome maintenance deficient) binding protein (Mcmbp)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $598.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mcmbp or NM_145955"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcmbp
Synonyms 1110007A13Rik; AU017382; BC025641; MCM-BP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209710 representing NM_145955
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGTGGAGAAGATTGGCTGAGCCACCCGCTCGGGATCGTGCAAGGCTTCTTCGCACAAAATGGAG
TTAATCCTGACTGGGAGAAGAAGGTTATTGAATACTTCAAGGAAAAGCTGAAGGAAAATAATGCTCCTAA
ATGGGTGCCATCGCTGAACGAAGTGCCCCTCCATTATCTGAAGCCCAACAGCTTTGTCAAGTTTCGTTGC
ATGATTCAGGATATGTTTGACCCTGAGTTTTACATGGGAATTTATGAGACAGTTAACCAGAACACAAAAG
CACGCGTTCTTCATTTTGGAAAATACAGAGATGTAGCAGAATGTGGGCCTCAACAAGAACTTGACTTGAG
CTCTCCAAGGTCTACCACTTCGGAAAGACAGACCTTCTACTGTGTCCCCGTGCCCGGGGAGTCTTCGTGG
GTCAAAGAGGCCTATGTCAATGCGAACCAAGCTCGAGTCAGTCCCTCGACCTCCTACACTCCCAGTAGGC
ACAAGAGGAGTTATGAAGATGACGAGGACATGGACCTACAGCCCAGTAAACAGAAGGAGCAGCATCCAGG
CTCCCGGCAAGCAGGAGGCCTCGGGGGCCTTCACTGGTGCGGAGAGCCAAAACGCTTAGAAACTGAAGCT
TCCTCTGGACAGCAGCTGAACACCTTGAACCTCTCTTCCCCGTTTGATCTGAACTTTCCTCTGCCAGGAG
AAAAGGGTCCTGCCTGCCTTGTTAAGGTCTACGAAGATTGGGATTGCTTCAAAGTCAATGATGTCCTTGA
GTTATACGGTGTGCTCTCTGTGGATCCTGTCCTGAGCGTACTGAATAGTGAGGAAAGGGATGCCTCTGCA
CTGCTGGACCCCATGGAGTGCACAGACATGGCTGAAGAGCAGAGAGTGCACAGCCCACCCGCTTCACTAG
TGCCAAGAATTCATGTGATTTTAGCTCAGAAGTTGCAGCACATCAATCCGTTGTTACCCACTTGCCTTAA
CAAGGAGGAGAGCAGAAGCTGTCAGTTTGTGTCCAATTTCATGTCTGAGTTGTCTCCAGTCAGAGCAGAG
CTGCTTGGATTCCTCACTCACGCACTCTTGGGAGATAGTTTGGCTGCTGAATATCTTATATTGCATCTCA
TCTCTACAGTGTATACAAGAAGAGATGTACTTCCTCTAGGAAAATTTACAGTTAACCTGAGTGGTTGCCC
GCAGAACAGTACCTTCACGGAACACCTCTACCGGATCATCCAACATCTTGTCCCAGCATCTTTTCGTCTA
CAGATGACAATAGAAAACATGAACCAGTTGAAGCTCATTCCCCACAAAGACTACACAGCCAACCGACTGG
TCAGTGGCCTCCTTCAGCTGCCCAACAATACTTCCCTGGTCATTGACGAGACTCTTCTGGAGCAAGGGCA
GTTGGACACGCCAGGTGTTCATAACGTAACTGCCCTGAGCAACCTGATAACCTGGCAGAAGGTGGATTAC
GACTTCAGCTATCACCAGATGGAGTTCCCATGCAATATCAATGTTCTCATCACATCTGAGGGAAGGTCAC
TACTTCCGGCAGACTGCCAGATTCACCTGCAGCCCCAGCTGATACCACCCAACATGGAGGAGTACATGAA
CGGCCTGCTCTCAGCTGTGCTGCCGTCCGTGCTCAACAAGTTCCGGATCTACCTGACCTTGTTGAGATTC
CTGGATTACAACCTATCAGACGACATAACCAAGGCGGTTGAAGATGATTTTGTGGAAATGCGTAAAGATG
ACCCTCAGAGCATCACGGCTGATGACCTCCACCAGCTGCTTGTGGTGGCTCGATTCCTGTCTCTGAGCGT
CGGTCAGACAACACTCTCCAGAGAGCGCTGGCTCAGAGCAAAGCAGCTGGAGCTCTCCAGGAAGGCCCGG
CTTCAGCAGCAGAAGAGTGTGAATGGGAACGAGCTT

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209710 representing NM_145955
Red=Cloning site Green=Tags(s)

MPCGEDWLSHPLGIVQGFFAQNGVNPDWEKKVIEYFKEKLKENNAPKWVPSLNEVPLHYLKPNSFVKFRC
MIQDMFDPEFYMGIYETVNQNTKARVLHFGKYRDVAECGPQQELDLSSPRSTTSERQTFYCVPVPGESSW
VKEAYVNANQARVSPSTSYTPSRHKRSYEDDEDMDLQPSKQKEQHPGSRQAGGLGGLHWCGEPKRLETEA
SSGQQLNTLNLSSPFDLNFPLPGEKGPACLVKVYEDWDCFKVNDVLELYGVLSVDPVLSVLNSEERDASA
LLDPMECTDMAEEQRVHSPPASLVPRIHVILAQKLQHINPLLPTCLNKEESRSCQFVSNFMSELSPVRAE
LLGFLTHALLGDSLAAEYLILHLISTVYTRRDVLPLGKFTVNLSGCPQNSTFTEHLYRIIQHLVPASFRL
QMTIENMNQLKLIPHKDYTANRLVSGLLQLPNNTSLVIDETLLEQGQLDTPGVHNVTALSNLITWQKVDY
DFSYHQMEFPCNINVLITSEGRSLLPADCQIHLQPQLIPPNMEEYMNGLLSAVLPSVLNKFRIYLTLLRF
LDYNLSDDITKAVEDDFVEMRKDDPQSITADDLHQLLVVARFLSLSVGQTTLSRERWLRAKQLELSRKAR
LQQQKSVNGNEL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_145955
ORF Size 1926 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145955.3, NP_666067.1
RefSeq Size 4171 bp
RefSeq ORF 1929 bp
Locus ID 210711
UniProt ID Q8R3C0
Cytogenetics 7 F3
MW 72.9 kDa
Summary Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion (By similarity).UniProtKB/Swiss-Prot Function
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"NM_145955" in other vectors (4)
SKU Description Size Price
MC201757 Mcmbp (untagged) - Mouse MCM (minichromosome maintenance deficient) binding protein (Mcmbp), (10ug) 10 ug
$598.00
MG209710 Mcmbp (tGFP-tagged) - Mouse RIKEN cDNA 1110007A13 gene (1110007A13Rik) 10 ug
$798.00
MR209710L3 Lenti ORF clone of Mcmbp (Myc-DDK-tagged) - Mouse MCM (minichromosome maintenance deficient) binding protein (Mcmbp) 10 ug
$898.00
MR209710L4 Lenti ORF clone of Mcmbp (mGFP-tagged) - Mouse MCM (minichromosome maintenance deficient) binding protein (Mcmbp) 10 ug
$898.00

Plasmid Map for MR209710

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209710
Circular map for MR209710

file-alt Citations

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