Ecd (NM_027475) Mouse Tagged ORF Clone
$289.00
$598.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ecd |
| Synonyms | 5730461K03Rik; Hsgt1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_027475 |
| ORF Size | 1923 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_027475.3 |
| RefSeq Size | 3125 bp |
| RefSeq ORF | 1926 bp |
| Locus ID | 70601 |
| UniProt ID | Q9CS74 |
| Cytogenetics | 14 A3 |
| MW | 71.7 kDa |
| Summary | Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (By similarity). Involved in regulation of cell cycle progression (PubMed:26711270). Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_027475" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC201188 | Ecd (untagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd), (10ug) | 10 ug |
$598.00
|
|
| MG209694 | Ecd (tGFP-tagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd) | 10 ug |
$798.00
|
|
| MR209694L3 | Lenti ORF clone of Ecd (Myc-DDK-tagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd) | 10 ug |
$898.00
|
|
| MR209694L4 | Lenti ORF clone of Ecd (mGFP-tagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd) | 10 ug |
$898.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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