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Ecd (NM_027475) Mouse Untagged Clone

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SKU
MC201188
Ecd (untagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $598.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ecd
Synonyms 5730461K03Rik; Hsgt1
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC013470 sequence for NM_027475
GAGCCTCACGACTTGCAGGCGTTCAGGCGTGGATCAGGGCCCAAGCGAACGCGAGGACCCAGCATGGGCC GGGATTGGGCGGTTGGTGGTCGCCTCTGACCAAGTGTCCTGCTGGAAGAGGCAGCCAAGGAAAGGTGCCA GAGCAGAGGCGCACGAGAGAGCTATTGAAAGGGATCGCTTTACTTTTGTCAGGTGGAAGAAGAGCGTCTA GTTTGGTGAAGGAGCACTATACCGCGCCAGGACAGTGCTCCTGGTGGCTCCTCTTCTGTACTCTCGGAGA AGAATGGAGGGCAGTGGAAAGCTCGCAATGGTAGAGGATGCTGTGGAGTACCATCTCTTCCTGATACCAG ATAAAGCCAGAGGCACAGAAGAGCACAGAGAGATTCTTCAGAAGTACATTGAACGGATAATGACCCAGTT CGCACCCATACTGGTCCCTTATATCTGGCAGAATCAGCCTTTCAATCTCAAATACAAGCCTGCAAAAGGA GGTGTTCCTGCTCATATGTACGGCATGACAAAGTTTGGAGATAACATTGAGGATGAATGGTTTATTGTTT ATGTAATAAAGCAGATTACAAAGGAATTTCCAGAGTTAGTGGCCAGGGTTGAAGACAATGATGGTGAGTT CCTGTTAATAGAAGCGGCCGACTTTCTCCCTAAGTGGTTGGACCCTGATAACAGTGCCAACAGGGTGTTT TTTCATCATGGGGAGCTGTGCATTATTCCTGTGCCGAGGAAATCTGAAAGGATACCATGGTTACCCATGA CACCACCAACAATCCAGCAAGCTTTGAGTATAATCTCGGCACACCCAGAAGCTGTTTTGGCTTCAGAATC TATACAAGCTGCTGTGGACAGGCGCGTCAGTGGGTACCCAGAAAGGGTCGAAGCATCCCTCCATCGAGCA CACTGCTTCCTTCCAGCCGGCATTGTGGCGGTGCTCAAGCAACAGCCCAGGCTGCTGTCGGCAGCCGTCC AGGCCTTCTACCTACGGGACCCCATTGACCTAAGAGCCTGTCGTGTCTTCAAGACATTTTTGCCTGAAAC ACGAATAATGGCCTCGGTCACTTTCACGAAATGTCTGTACGCACAGCTGGTACAGCAGAAGTTTGTGCCA GACCGGCGGAGTGGATACGGGCTCCCACCTCCATCACATCCCCAGTACCGAGCTTATGAACTGGGCATGA AGTTGGCTCATGGATTTGAGATTTTGTGCTCCAAATGTAGCCCACATTTTTCTGACTCCAGGAAGTCTCT TGTGACTGCCTCACCGCTCTGGGCCAGCTTCCTTGAAAGTCTGAAAAGGAATGACTACTTTAAGGGACTG ATGGACGGCTCTGCGCAGTACCAGGAGAGACTAGAAATGGCAAAGAACTATTTCCAGCTCTCTATACACC GGCCAGAAAGTTCACTTGCTATGAGCCCAGGCGAAGAGATCCTAACCGTGTTACAGACACAGCCATTTGA CGTCGCCGAGCTCAAGACAGAAGAAGCTGACCTTCCTCCGGAGGACGATGACCAGTGGTTAGACCTCTCA CCAGACCAGTTAGACCAGCTACTGCAAGATGCTGCGGGCAGAAAAGAATCCCAGCCCGGTCCCCAGAAGG AGGAGCTGCAGAACTATGATGTAGCGCAGGTCTCAGACAGCATGAAGGCCTTCATCTCCAAAGTGTCTAG CCACAAGGGGGCAGAGCTGCCCAGAGACCCTTCGGAAGCTCCAATCACTTTTGATGCAGATTCTTTTCTG AATTATTTTGATAAGATTTTAGGAGCAAAGCCTCAGGAGTCAGACTCTGAGGATGACCCGGGTGAGGAGG ACGTGGAAGGTGTAGACAGTGATGATGACGTGGGCTTTGAGGCTCAGGAGTCTGAGTCTCTGAAAGGAGC CCTTGGCAGCCTCAAGTCCTACATGGCCCGGATGGACCAGGAACTGGCACACACCAGCATGGGCAGAAGC TTCACCACGCGGGAGCGATTGAATAAGGACCCTCCATCCCACACTGCAAATGACAATTCAGATGAAGAAG ATTCTGGTGCAGGAGATTGCGCTGTTGAAGCAGTGGATGTAGACCTGAACCTGATTTCAAATATACTGGA ATCCTACAGCTCCCAAGCGGGCCTGGCAGGACCTGCTTCCAATCTGTTACACAGCATGGGTGTGCGACTG CCCGACAACGCTGATCACAACCCCCAAGTCAGCCAGTGAAGGACTAGCCTGCACATCAGCCACCCTTCTC AGGAATAAACGACTCTTCATAAGCACGCCTTTAATCCCAGCACTCGGGAGACAGAGGCAGGCGGATTTCT GAGTTCGAGGCCAGCCTGGTCTAGAAAATGAGTTCCAGGAGAGCCAGGGCTACACAGAGAAACCCTGTCT CAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI |
ACCN NM_027475
Insert Size 1926 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC013470, AAH13470
RefSeq Size 2407 bp
RefSeq ORF 1926 bp
Locus ID 70601
UniProt ID Q9CS74
Cytogenetics 14 A3
Summary Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (By similarity). Involved in regulation of cell cycle progression (PubMed:26711270). Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027475" in other vectors (4)
SKU Description Size Price
MG209694 Ecd (tGFP-tagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd) 10 ug
$798.00
MR209694 Ecd (Myc-DDK-tagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd) 10 ug
$289.00 $598.00
MR209694L3 Lenti ORF clone of Ecd (Myc-DDK-tagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd) 10 ug
$898.00
MR209694L4 Lenti ORF clone of Ecd (mGFP-tagged) - Mouse ecdysoneless homolog (Drosophila) (Ecd) 10 ug
$898.00

file-alt Citations

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