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Rhot1 (NM_021536) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209606
Rhot1 (Myc-DDK-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $588.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rhot1
Synonyms 2210403N23Rik; AA415293; AF244542; AI834919; Arht1; C430039G08Rik; Miro1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209606 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTGCGGGCCGAGTGCGGCCCCTGCGAGCCTCCGACATGAAGAAAGACGTGAGGATCCTGCTGGTGG
GAGAACCTCGAGTTGGGAAGACATCACTGATTATGTCGCTGGTCAGTGAAGAATTTCCGGAAGAGGTTCC
TCCCCGAGCAGAAGAAATCACCATTCCAGCTGACGTCACCCCTGAAAGAGTTCCAACACACATTGTCGAT
TATTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCACAGGCTAATGTTATCTGCATAG
TTTATGCTGTTAACAACAAACATTCTATTGATAAGGTAACAAGCCGATGGATTCCTCTCATAAATGAAAG
AACAGACAAAGACAGCAGGCTGCCTTTGATATTGGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGT
ATGGAGACCATCCTTCCAATTATGAACCAGTATACAGAGATAGAGACCTGTGTGGAGTGTTCAGCAAAAA
ACCTAAAGAATATCTCAGAGCTGTTTTATTATGCACAGAAAGCTGTTCTTCATCCTACAGGGCCTCTATA
CTGCCCAGAGGAGAAAGAGATGAAACCGGCTTGTATAAAGGCCCTCACTCGTATATTTAAAATATCTGAT
CAGGATAACGACGGCACTCTGAATGATGCTGAGCTCAACTTCTTCCAGAGAATTTGTTTCAACACTCCTT
TAGCTCCGCAAGCGCTGGAAGATGTCAAGAATGTGGTTAGAAAGCACTTAAGTGACGGTGTGGCTGACAG
TGGGCTGACGCTCAGAGGTTTTCTTTTTTTACATACACTTTTTATCCAGAGGGGGAGGCATGAGACTACT
TGGACTGTGCTTCGACGATTTGGTTATGACGATGACCTGGACCTGACGCCTGAGTATTTATTCCCCCTGC
TAAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTGTTTCTCCAGAGCACCTTTGA
CAAACATGATTTGGACAGAGACTGTGCTTTGTCGCCTGATGAACTTAAAGATTTATTTCAAGTTTTTCCT
TACATACCTTGGGGACCAGATGTAAATAACACAGTTTGCACAAATGAAAGAGGCTGGATAACCTACCAGG
GGTTCCTCTCCCAGTGGACGCTCACCACCTACTTAGATGTGCAGCGTTGCCTGGAGTATTTGGGATATCT
AGGCTATTCCATACTGACGGAACAAGAGTCTCAAGCTTCAGCCATTACAGTAACAAGGGATAAAAAGATT
GACCTGCAGAAAAAACAGACTCAGAGAAATGTGTTCAGGTGTAATGTAATTGGAGTGAAAGGCTGTGGGA
AAACTGGAGTTCTTCAGTCTCTCCTTGGAAGAAACCTAATGAGGCAGAAGAAAATTCGTGATGATCATAA
GTCCTATTATGCAATTAACACTGTCTATGTATATGGACAAGAGAAATACTTGTTGCTGCACGATATCTCA
GAGTCGGAATTTCTAACTGAGGCTGAGACCATTTGTGATGTTGTATGCCTGGTATATGATGTCACCAATC
CCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACATTTTATGGACAGCAGAATTCCCTGCTTGAT
TGTAGCTGCAAAGTCAGACCTGCATGAAGTCAAACAGGAACATAGCATCTCACCTACTGATTTCTGCAGG
AAACACAAAATGCCTCCACCTCAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTG
TTAAGTTGACAACAATGGCCATGTACCCGCACGTGACACAAGCTGACCTCAAGAGCTCAACGTTCTGGCT
TCGCGCAAGCTTTGGTGCTACGGTCTTTGCAGTCGTGGGCTTTGCTATGTACAGAGCACTACTGAAACAG
CGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209606 protein sequence
Red=Cloning site Green=Tags(s)

MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVD
YSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSS
METILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISD
QDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETT
WTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFP
YIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKI
DLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDIS
ESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCR
KHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQ
R

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021536
ORF Size 1893 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021536.3, NM_021536.4, NM_021536.5, NM_021536.6, NM_021536.7, NP_067511.4
RefSeq Size 4137 bp
RefSeq ORF 1896 bp
Locus ID 59040
UniProt ID Q8BG51
Cytogenetics 11 47.62 cM
MW 72.2 kDa
Summary Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_021536" in other vectors (4)
SKU Description Size Price
MC202696 Rhot1 (untagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1, (10ug) 10 ug
$588.00
MG209606 Rhot1 (tGFP-tagged) - Mouse ras homolog gene family, member T1 (Rhot1) 10 ug
$788.00
MR209606L3 Lenti ORF clone of Rhot1 (Myc-DDK-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1 10 ug
$888.00
MR209606L4 Lenti ORF clone of Rhot1 (mGFP-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1 10 ug
$888.00

Plasmid Map for MR209606

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209606
Circular map for MR209606

file-alt Citations

PRDX5 Regulates Mitochondrial Function and Nuclear Spreading in Myogenesis and Acts With PRDX3 to Delay Muscle Aging
Suh, Eom, Baik et al
J Cachexia Sarcopenia Muscle (2025) 16 (6), e70098 DOI: 10.1002/jcsm.70098
Cited by 3
AI Summary: OriGene’s pCMV-Rhot1 (MR209606) and pCMV-Trak1 (MR209421) plasmids were used in this study to investigate the roles of Rhot1 and Trak1 in mitochondrial transport and nuclear positioning during myogenesis, helping to demonstrate how PRDX5 regulates these processes in muscle cells.
Snippet: nk, Medical Genomics Research center, KRIBB, Korea. The pCMV‐mCherry‐Lifeact plasmid was kindly provided by Jin Man Kim (Seoul National University, Korea). pCMV‐Rhot1 and pCMV‐Trak1 plasmids (Origene;MR209606 and MR209421, respectively) were transfected using Lipofectamine 3000 (Invitrogen) reagent. For siRNA transfection, Lipofectamine RNAiMAX (Invitrogen) transfection reagent was used
FigureFigureFigure
Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2
Covill-Cooke, Toncheva, Drew et al
EMBO Rep. (2020) 21 (2), e49865 DOI: 10.15252/embr.201949865
Cited by 52
AI Summary: OriGene products MR209606, MR224107, and MR224933 provided the mouse Miro1 splice variant cDNA clones used to generate GFP-tagged constructs, enabling the study of Miro1’s role in peroxisomal fission in this paper.
Snippet: and mycPex19 mouse Pex19 (NM_023041) cloned into pRK5‐myc vector. GFP‐tagged mouse Miro1 splice variants (GFPv1, GFPv2, GFPv3 and GFPv4) were cloned from NM_021536 (v1), NM_001163354 (v2) and NM_001163354 (v3) (OriGene: MR209606, MR224107 and MR224933, respectively) into pEGFP‐N1. …
Showing 1-2 of 2 papers.
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