OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Rhot1 (NM_021536) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG209606
Rhot1 (tGFP-tagged) - Mouse ras homolog gene family, member T1 (Rhot1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $788.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Rhot1 or NM_021536"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rhot1
Synonyms 2210403N23Rik; AA415293; AF244542; AI834919; Arht1; C430039G08Rik; Miro1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209606 representing NM_021536
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTGCGGGCCGAGTGCGGCCCCTGCGAGCCTCCGACATGAAGAAAGACGTGAGGATCCTGCTGGTGG
GAGAACCTCGAGTTGGGAAGACATCACTGATTATGTCGCTGGTCAGTGAAGAATTTCCGGAAGAGGTTCC
TCCCCGAGCAGAAGAAATCACCATTCCAGCTGACGTCACCCCTGAAAGAGTTCCAACACACATTGTCGAT
TATTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCACAGGCTAATGTTATCTGCATAG
TTTATGCTGTTAACAACAAACATTCTATTGATAAGGTAACAAGCCGATGGATTCCTCTCATAAATGAAAG
AACAGACAAAGACAGCAGGCTGCCTTTGATATTGGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGT
ATGGAGACCATCCTTCCAATTATGAACCAGTATACAGAGATAGAGACCTGTGTGGAGTGTTCAGCAAAAA
ACCTAAAGAATATCTCAGAGCTGTTTTATTATGCACAGAAAGCTGTTCTTCATCCTACAGGGCCTCTATA
CTGCCCAGAGGAGAAAGAGATGAAACCGGCTTGTATAAAGGCCCTCACTCGTATATTTAAAATATCTGAT
CAGGATAACGACGGCACTCTGAATGATGCTGAGCTCAACTTCTTCCAGAGAATTTGTTTCAACACTCCTT
TAGCTCCGCAAGCGCTGGAAGATGTCAAGAATGTGGTTAGAAAGCACTTAAGTGACGGTGTGGCTGACAG
TGGGCTGACGCTCAGAGGTTTTCTTTTTTTACATACACTTTTTATCCAGAGGGGGAGGCATGAGACTACT
TGGACTGTGCTTCGACGATTTGGTTATGACGATGACCTGGACCTGACGCCTGAGTATTTATTCCCCCTGC
TAAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTGTTTCTCCAGAGCACCTTTGA
CAAACATGATTTGGACAGAGACTGTGCTTTGTCGCCTGATGAACTTAAAGATTTATTTCAAGTTTTTCCT
TACATACCTTGGGGACCAGATGTAAATAACACAGTTTGCACAAATGAAAGAGGCTGGATAACCTACCAGG
GGTTCCTCTCCCAGTGGACGCTCACCACCTACTTAGATGTGCAGCGTTGCCTGGAGTATTTGGGATATCT
AGGCTATTCCATACTGACGGAACAAGAGTCTCAAGCTTCAGCCATTACAGTAACAAGGGATAAAAAGATT
GACCTGCAGAAAAAACAGACTCAGAGAAATGTGTTCAGGTGTAATGTAATTGGAGTGAAAGGCTGTGGGA
AAACTGGAGTTCTTCAGTCTCTCCTTGGAAGAAACCTAATGAGGCAGAAGAAAATTCGTGATGATCATAA
GTCCTATTATGCAATTAACACTGTCTATGTATATGGACAAGAGAAATACTTGTTGCTGCACGATATCTCA
GAGTCGGAATTTCTAACTGAGGCTGAGACCATTTGTGATGTTGTATGCCTGGTATATGATGTCACCAATC
CCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACATTTTATGGACAGCAGAATTCCCTGCTTGAT
TGTAGCTGCAAAGTCAGACCTGCATGAAGTCAAACAGGAACATAGCATCTCACCTACTGATTTCTGCAGG
AAACACAAAATGCCTCCACCTCAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTG
TTAAGTTGACAACAATGGCCATGTACCCGCACGTGACACAAGCTGACCTCAAGAGCTCAACGTTCTGGCT
TCGCGCAAGCTTTGGTGCTACGGTCTTTGCAGTCGTGGGCTTTGCTATGTACAGAGCACTACTGAAACAG
CGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209606 representing NM_021536
Red=Cloning site Green=Tags(s)

MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVD
YSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSS
METILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISD
QDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETT
WTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFP
YIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKI
DLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDIS
ESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCR
KHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGATVFAVVGFAMYRALLKQ
R

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021536
ORF Size 1893 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021536.6, NP_067511.4
RefSeq Size 4077 bp
RefSeq ORF 1896 bp
Locus ID 59040
UniProt ID Q8BG51
Cytogenetics 11 47.62 cM
Summary Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_021536" in other vectors (4)
SKU Description Size Price
MC202696 Rhot1 (untagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1, (10ug) 10 ug
$588.00
MR209606 Rhot1 (Myc-DDK-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1 10 ug
$289.00 $588.00
MR209606L3 Lenti ORF clone of Rhot1 (Myc-DDK-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1 10 ug
$888.00
MR209606L4 Lenti ORF clone of Rhot1 (mGFP-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 1 10 ug
$888.00

Plasmid Map for MG209606

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209606
Circular map for MG209606

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products