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Efcab7 (NM_145549) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209579
Efcab7 (Myc-DDK-tagged) - Mouse EF-hand calcium binding domain 7 (Efcab7)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $585.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Efcab7
Synonyms mKIAA1799
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209579 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCAACCCTGGAAGTGATGCAGCTTTGGGTACTCAGAACCCACTGCTTTCTGGGAGTCCTCGAA
CAAAGAAATTCCCACTAACTGAACAGGAGGTGTTTTATATGAACTGTAGAGCCGCCTACTTAACTATCTT
CAAAAGCAGCTTGGAAAACATTATTTCTAAAGATCAACTTTACCTAGCTCTTCAGCATGCAGGAAGAAAT
CCATCCCAAAAGACCATTAATAAGTATTGGACTCCTCAAACTGCCAAACTAAATTTTGATGATTTTTGTA
TAATTTTAAGCAAGGAAAAGCCTACTTCAAAAGCAGAATTGCTAAAATCATTTAAAAAATTAGATGTAAA
TGATGATGGTGCTATTCTACACTCTGACCTTCAGAAATATCTAACAAAGAGAGGTGAGAAGATGACTCAG
GAAGAAGTGAATGCTGTCATAAATTTGGCTGATATAAATGCTAATGGAAAGTTTGACTACGTTAAGTTTT
GTAAATTATACATGACAACCAGTGAGCAGTGTCTCAAGACTACACTAGAAAGACTGGAGGCCGACAGTAA
ACTGAGGCGTCAACAGTTTGGAAGCCACATGGAAGGGTCCCCTGAAAGGGGTCCGTCACCAGCACCAAAA
CCGTCACCTAGGGTCATAAGAAAAAATGACCAAGAAACATTCTCAAGCAAAGGTGACACAAGCCATGCCT
TACTGTCAACAACTAGAAAGTTCAAAACATCTGTCTCCTTCACAATGACCATGAGCGCTAACAGTAACCA
AGACTCAACATTAACTGAGCCAAACTTAAAGGATTGGCAGTGTGCACAGTCAAAAGGCTGCTTCTTTTTA
GAAGAAGACGGTGAAGTTGTCAGTCATCAGTACAAAATGCACATAGCTCAGAGATCTGTGCTTTACCTAA
CAATTAAGCCGTTATATCTGAGTCAAGTGGAAGGAAAACGTTGCCCCTGGTTATCGGTTGACACAGCCTT
ATATATTCTTAAGAAAAATGAGAATCCAGCAGAGCCACAGCTCATGTGCTTTACTGAACTACGAAACAGA
GAAGTGTTTGGATGGACTGGTGAACTAGAAGCAGGAATTTATTGGTTAATTCCTTCCACAACTGGCTGTA
GGCTAAAAAAAGAAACAAAACCAGTAACAGAAGAAGCCCAGCTTGTACATAGAGATGAAACAGGGGAATT
ATCTCTTACAAGTGAGTTTAGGTCAACTTTATCAGAGATATTTGAAGTAATTGATTTAGATGGCAATGGT
CTGATTAGCCTTGAAGAGTACAATTTTTTTGAATTGAGAACAAGTGGTGAGAAGTGTGATGAGGATGCCT
GGGCTGTCTGCAGAGAAAACTTTGACACAAAGAAGAATGAATTAACAAGACAGGGCTTTATGGACTTGCA
TCTGATGGAAGCAAATGACCGAGAAGGAGACCCTCTTGACCTGTGGGTGACTCTGCATTCCATGGGCTAT
AATAAGGCTCTGGAGCTGACAGAGGCATGCCCATTTGTCATCAACATCTACGCTGAAAGATGCAAGCCAA
GAATCAAAGTTGTCCATATGGAGGCATGCAGTGGGCAACTTGAAAAGGCCATTTGCAAATCTGTCCTTGA
TAGAAGCGATGCTAAAGTCATGGATGGCTATGAAAATATAATCGTACATACTTGCAATTATGACACATGG
ATAACATCAATTATTGAAAATAAGTCAGATAATAAAGTGATTATTCACATCAATAACGAACTGAGTAAAA
ACTGTGTCAACAACAGAGGACTCAACATCTTTGCAGTAGAAGTGGCACCAAGATCTACAATGGTGTGTCA
ACATGTAATGCCCCTGAATGAACAAGAAGAATGGATATATTGTTGTGTGTATTCTCTTGTAGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209579 protein sequence
Red=Cloning site Green=Tags(s)

MASNPGSDAALGTQNPLLSGSPRTKKFPLTEQEVFYMNCRAAYLTIFKSSLENIISKDQLYLALQHAGRN
PSQKTINKYWTPQTAKLNFDDFCIILSKEKPTSKAELLKSFKKLDVNDDGAILHSDLQKYLTKRGEKMTQ
EEVNAVINLADINANGKFDYVKFCKLYMTTSEQCLKTTLERLEADSKLRRQQFGSHMEGSPERGPSPAPK
PSPRVIRKNDQETFSSKGDTSHALLSTTRKFKTSVSFTMTMSANSNQDSTLTEPNLKDWQCAQSKGCFFL
EEDGEVVSHQYKMHIAQRSVLYLTIKPLYLSQVEGKRCPWLSVDTALYILKKNENPAEPQLMCFTELRNR
EVFGWTGELEAGIYWLIPSTTGCRLKKETKPVTEEAQLVHRDETGELSLTSEFRSTLSEIFEVIDLDGNG
LISLEEYNFFELRTSGEKCDEDAWAVCRENFDTKKNELTRQGFMDLHLMEANDREGDPLDLWVTLHSMGY
NKALELTEACPFVINIYAERCKPRIKVVHMEACSGQLEKAICKSVLDRSDAKVMDGYENIIVHTCNYDTW
ITSIIENKSDNKVIIHINNELSKNCVNNRGLNIFAVEVAPRSTMVCQHVMPLNEQEEWIYCCVYSLVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145549
ORF Size 1884 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145549.1, NP_663524.1
RefSeq Size 2132 bp
RefSeq ORF 1887 bp
Locus ID 230500
UniProt ID Q8VDY4
Cytogenetics 4 C6
MW 71.4 kDa
Summary Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (PubMed:24582806). Required for the localization of the EVC2:EVC subcomplex at the base of primary cilia (PubMed:24582806).UniProtKB/Swiss-Prot Function
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"NM_145549" in other vectors (4)
SKU Description Size Price
MC203112 Efcab7 (untagged) - Mouse EF-hand calcium binding domain 7 (Efcab7), (10ug) 10 ug
$585.00
MG209579 Efcab7 (tGFP-tagged) - Mouse cDNA sequence BC020077 (BC020077) 10 ug
$785.00
MR209579L3 Lenti ORF clone of Efcab7 (Myc-DDK-tagged) - Mouse EF-hand calcium binding domain 7 (Efcab7) 10 ug
$885.00
MR209579L4 Lenti ORF clone of Efcab7 (mGFP-tagged) - Mouse EF-hand calcium binding domain 7 (Efcab7) 10 ug
$885.00

Plasmid Map for MR209579

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209579
Circular map for MR209579

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