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Ccdc22 (NM_138603) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209570
Ccdc22 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 22 (Ccdc22)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $585.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccdc22
Synonyms AI481216; DXImx40e; Ppp1r3f; Sfc22
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209570 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGCTGACCGAATCCTCATCCATTCCTTGCGCCAGGCGGGCACGGCAGTTCCTCCAGAAGTGC
AGACTCTGCGAGCTTTCACCACTGAGCTGGTCGTAGAGGCTGTGGTCCGATGCCTTCGAGTGATTAATCC
TGATGTGGGCTCTGGCCTCAGTCACTTGCTACCACCAGCCATGTCTGCCCGCTTCCGCCTGGCCATGAGT
CTGGCCCAGGCCTGCATGGACCTGGGCTATCCCTTGGAGCTTGGCTATCAGAACTTCCTCTATCCCAGTG
AGCCTGACCTTCGAGATCTGCTTCTTTTCTTGGCTGAGCGACTACCCAGCGATGCCTCTGAGGACGCAGA
CCAGCCTGCAGGTGACTCGGCTATTTTCCTTCGGGCCATTGGGAGCCAAATCCGGGACCAGCTGGCCCTG
CCCTGGGTCCCACCTCTCCTTCGAACTCCCAAGGTGCAGCGCCTCCAGGGCTCAGCCCTCCAGCAGCCGT
TCCACTCCAGCAGGTTGGTTCTACCTGAGTTGAATTCCAGCGGAGAGCTATGGGAGTTCCAGGCCAGTCC
CCTGCTGCTACCAGCCCCCACACAGGTGCCCCAGCTTCAGGGGAGGGCAGCCTCACTCCTTGAACACCAT
GCCAGCCAGCTCTGCCAGCATGTGAACAGGGATTGCCCAGGAGATGAAGACCGGGTGCGCTGGGCATCCC
GCGTCCCATCCCAGGAGGATTCCCGGGCTCCACAGCAGAGACTGCACAAGCAGCTGATTGAACATCTGCG
TCAAAGCTGGGGCCCACTTGGAGCCCCGACACAAGTCCGAGACCTAGGAGAGATGCTGCAGACCTGGGGT
GCTAGGGCTATGACTGGTGTGCCCAAAGGCTCCCGCTTCACCCATTCAGAGAAGTTTACCTTCCATCTGG
AGCCCCAGGTCCAGGCAGCTCAGGTGGCAGATGTACCAGCTACCTCTCAAAGGCTTGAACAGGACACACG
GGCAGCTCAAGAGCAGGAGTTAGAGTCCCTTCGTGAGCAGCTGGCGAGTGTGAACCACAACATTGAAGAG
GTTGAAGCTGACATGAAGACCCTGGGAATCAACCTTGTGCAGGTGGAAACCGAGTGCCGACAAAGTGAAC
TCAGTGTGGCCGAGCAGGAGCAGGCCCTGCGCCTGAAGAGCAGGACGGTGGAATTGCTGCCTGATGGGGC
TGCCAACCTCGCCAAACTGCAGCTTGTGGTGGAGAGCAGCGCTCAGAGGCTCATCCATCTAGCAAGCCAG
TGGGAGAAACACCGGGTCCCACTTCTTGCTGAGTACCGCCACCTCAGAAGACTCCAGGACTGTAGGGAGC
TGGAATCGTCTCGACGGCTGGCAGAAATCCAGGAGCTGCACCACAGTGTTCGAGCAGCTGCAGAGGAGGC
TCGCCGGAAGGAGGAGGTCTATAAGCAGCTGGTGTCAGAGCTGGAGACTCTGCCAAAAGATGTGTCCCGG
CTGGCCTATACTCAGCGCATCCTGGAGATTGTGGGCAACATCCGGAAGCAGAAGGAAGAGATCACTAAGA
TCTTGTCGGACACAAAGGAACTGCAGAAGGAAATCAACTCTCTCTCTGGGAAGCTCGACCGGACATTTGC
AGTGACTGATGAGCTTGTGTTCAAGGATGCTAAGAAGGATGATGCTGTTCGGAAAGCCTACAAGTACCTA
GCTGCCCTGCATGAGAATTGCAGTCAGCTCATCCAGACCATTGAGGACACAGGCACTATCATGAGGGAAG
TTCGAGATCTTGAGGAGCAGATTGAGACAGAGATGGGCAAGAAGACTTTGAGCAACCTGGAGAAGATCTG
TGAGGACTACCGAGCCCTTCGCCAGGAGAATGCTGGCCTCTTGGGCCGGGTCCGAGAGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209570 protein sequence
Red=Cloning site Green=Tags(s)

MEEADRILIHSLRQAGTAVPPEVQTLRAFTTELVVEAVVRCLRVINPDVGSGLSHLLPPAMSARFRLAMS
LAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPSDASEDADQPAGDSAIFLRAIGSQIRDQLAL
PWVPPLLRTPKVQRLQGSALQQPFHSSRLVLPELNSSGELWEFQASPLLLPAPTQVPQLQGRAASLLEHH
ASQLCQHVNRDCPGDEDRVRWASRVPSQEDSRAPQQRLHKQLIEHLRQSWGPLGAPTQVRDLGEMLQTWG
ARAMTGVPKGSRFTHSEKFTFHLEPQVQAAQVADVPATSQRLEQDTRAAQEQELESLREQLASVNHNIEE
VEADMKTLGINLVQVETECRQSELSVAEQEQALRLKSRTVELLPDGAANLAKLQLVVESSAQRLIHLASQ
WEKHRVPLLAEYRHLRRLQDCRELESSRRLAEIQELHHSVRAAAEEARRKEEVYKQLVSELETLPKDVSR
LAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTDELVFKDAKKDDAVRKAYKYL
AALHENCSQLIQTIEDTGTIMREVRDLEEQIETEMGKKTLSNLEKICEDYRALRQENAGLLGRVREA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138603
ORF Size 1881 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138603.3, NP_613069.3
RefSeq Size 2274 bp
RefSeq ORF 1884 bp
Locus ID 54638
UniProt ID Q9JIG7
Cytogenetics X 3.42 cM
MW 70.8 kDa
Summary Involved in regulation of NF-kappa-B signaling. Promotes ubiquitination of I-kappa-B-kinase subunit IKBKB and its subsequent proteasomal degradation leading to NF-kappa-B activation; the function may involve association with COMMD8 and a CUL1-dependent E3 ubiquitin ligase complex. May down-regulate NF-kappa-B activity via association with COMMD1 and involving a CUL2-dependent E3 ubiquitin ligase complex. Regulates the cellular localization of COMM domain-containing proteins, such as COMMD1 and COMMD10. Component of the CCC complex, which is involved in the regulation of endosomal recycling of surface proteins, including integrins, signaling receptor and channels. The CCC complex associates with SNX17, retriever and WASH complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGA5:ITGB1. Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans-Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes.UniProtKB/Swiss-Prot Function
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"NM_138603" in other vectors (4)
SKU Description Size Price
MC205725 Ccdc22 (untagged) - Mouse coiled-coil domain containing 22 (Ccdc22), (10ug) 10 ug
$585.00
MG209570 Ccdc22 (tGFP-tagged) - Mouse coiled-coil domain containing 22 (Ccdc22) 10 ug
$785.00
MR209570L3 Lenti ORF clone of Ccdc22 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 22 (Ccdc22) 10 ug
$885.00
MR209570L4 Lenti ORF clone of Ccdc22 (mGFP-tagged) - Mouse coiled-coil domain containing 22 (Ccdc22) 10 ug
$885.00

Plasmid Map for MR209570

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file-download TXT (FASTA format)

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Cloning scheme for MR209570
Circular map for MR209570

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