OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Parn (NM_028761) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR209527
Parn (Myc-DDK-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $582.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA329951 Rabbit Polyclonal Anti-PARN Antibody
100 ul
$525.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Parn or NM_028761"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Parn
Synonyms 1200003I18Rik; DAN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATAATCCGGAGCAATTTTAAGATTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCTGACT
TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGCGATGGACCCTCAGTAACAGCATTAACAAGTGGTTT
TGACACCCCAGAAGAGAGATATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTGTTTCAGTTTGGC
CTTTGTGCTTTTAAGTATGACCACACAGATTCCAAGCATGTAACGAAGTCATTTAACTTCTATGTTTTCC
CCAAGCCTTTCAGTAGGTCCTCACCAGATGTCAAGTTTGTTTGCCAGAGCTCCAGCATTGACTTCCTGGC
GAGCCAAGGATTTGACTTTAATAAAGTGTTTTGCAGTGGGATTCCATATCTGAATCAGGAAGAAGAAAGG
CAGCTGAGAGAGCAATTCGATGAAAAACGGTCTCAAGCCAATGGGGCAGGAGCTCTGGCAAAATGTCCTG
TGACCATCCCTGAGGATCAGAAGAAGTTTATTGACCAAGTAATAGAGAAGATAGAGGATTTCCTACAAAG
TGAAGAGAAGAGGAGCCTGGAACTTGACCCATGCACTGGGTTCCAAAGAAAACTTATTTATCAGACTTTG
AGTTGGAAGTATCCTAAAGGCATTCATGTTGAGACATTAGAGACTGACAAGAAGGAAAGACATATAGTTA
TCAGCAAGGTGGATGAAGAAGAACGCAAAAGGAGAGAGCAGGAGAAGTATACAAAGGAACAGGAGGAGCT
GAATGATGCTGTGGGGTTTTCAAGAGTCATCCATGCCATTGCTAATTCGGGGAAGCTGGTTGTGGGACAC
AACATGCTCTTGGATGTCATGCATACGATTCATCAGTTCTACTGCCCCCTGCCTGCGGACCTGAATGAGT
TTAAGGAGATGGCAATATGTGTCTTCCCCAGACTCTTGGATACTAAGTTGATGGCCAGCACACAGCCTTT
TAAGGATATCATTAACAACACATCCCTTGCAGAGTTGGAAAAGCGGTTGAAAGAGACACCTTTTGACCCT
CCCAAAGTTGAAAGTGCAGAAGGCTTTCCAAGCTACGACACAGCTTCTGAGCAGCTTCATGAGGCGGGGT
ACGATGCCTACATCACAGGGCTCTGCTTCATCTCCATGGCAAATTACTTAGGTTCTTTACTCAGTCCTCC
AAAAATGTGTGTGTCTGCCAGATCAAAGCTCATTGAACCCTTTTTTAACAAGTTATTTCTTATGAGGGTC
ATGGATATTCCCTATTTAAACTTGGAAGGGCCAGACTTGCAGCCTAAGCGGGACCATGTTCTCCACGTGA
CCTTCCCCAAAGAGTGGAAAACCAGCGACCTGTACCAGCTCTTCAGCGCCTTCGGTAACATTCAGATATC
CTGGATTGATGATACATCAGCCTTCGTTTCTCTCAGCCAGCCAGAACAAGTACAAATTGCCGTTAATACC
AGCAAGTACGCTGAAAGTTATCGGATCCAGACCTATGCTGAGTATGTGGGAAAGAAGCAGGAAGGCAAGC
AGGTCAAGAGGAAGTGGACAGAAGACAGTTGGAAGGAGGTGGACAGAAAGCGGCCCCACATGCAGGGCCC
CTGTTACCACAGCAACAGCTTCACAGCAGCTGGCGTGCTTGGAAAGAGAACGCTGAGTCCTGACCCAAGG
GAAGCTGCCTTGGAGGACAGAGAATCAGAGGAGGTATCTGACTCGGAGCTTGAACAGACAGATTCCTGTA
CAGACCCCCTCCCAGAGGGAAGGAAAAAGTCCAAGAAGTTAAAACGAATGAAGAAGGAGCTTTCCTTGGC
AGGAAGTGTCTCGGATAGCCCTGCCGTGCTCTTTGAAGTCCCTGACACATGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209527 protein sequence
Red=Cloning site Green=Tags(s)

MEIIRSNFKINLHKVYQAIEEADFFAIDGEFSGISDGPSVTALTSGFDTPEERYQKLKKHSMDFLLFQFG
LCAFKYDHTDSKHVTKSFNFYVFPKPFSRSSPDVKFVCQSSSIDFLASQGFDFNKVFCSGIPYLNQEEER
QLREQFDEKRSQANGAGALAKCPVTIPEDQKKFIDQVIEKIEDFLQSEEKRSLELDPCTGFQRKLIYQTL
SWKYPKGIHVETLETDKKERHIVISKVDEEERKRREQEKYTKEQEELNDAVGFSRVIHAIANSGKLVVGH
NMLLDVMHTIHQFYCPLPADLNEFKEMAICVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFDP
PKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSLLSPPKMCVSARSKLIEPFFNKLFLMRV
MDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQVQIAVNT
SKYAESYRIQTYAEYVGKKQEGKQVKRKWTEDSWKEVDRKRPHMQGPCYHSNSFTAAGVLGKRTLSPDPR
EAALEDRESEEVSDSELEQTDSCTDPLPEGRKKSKKLKRMKKELSLAGSVSDSPAVLFEVPDTW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028761
ORF Size 1872 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028761.1, NM_028761.2, NM_028761.3, NP_083037.1
RefSeq Size 2902 bp
RefSeq ORF 1875 bp
Locus ID 74108
UniProt ID Q8VDG3
Cytogenetics 16 A1
MW 71.6 kDa
Summary 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3' UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization (By similarity). Also able to recognize poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to leading to microRNAs degradation or snoRNA increased stability (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_028761" in other vectors (4)
SKU Description Size Price
MC203133 Parn (untagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn), (10ug) 10 ug
$582.00
MG209527 Parn (tGFP-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn) 10 ug
$782.00
MR209527L3 Lenti ORF clone of Parn (Myc-DDK-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn) 10 ug
$882.00
MR209527L4 Lenti ORF clone of Parn (mGFP-tagged) - Mouse poly(A)-specific ribonuclease (deadenylation nuclease) (Parn) 10 ug
$882.00

Plasmid Map for MR209527

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209527
Circular map for MR209527

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products