Prickle3 (NM_175097) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR209523
Prickle3 (Myc-DDK-tagged) - Mouse prickle homolog 3
$289.00 $582.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prickle3
Synonyms Lmo6; Sfc16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209523 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCGCGCGTGGGTCCCGGAGGCGCCGCTCTGGGCGTGCGCCTCCAGAGGCAGAGGACCCTGCCCGCG
GCCAGCCTTGCAATTCCTGCAGGGAACAGTGCCCAGGCTTCCTGCTGCATGGCTGGAGAAAGATCTGCCA
GCACTGCAAATGCCCGCGGGAGGAGCATGCTGTACGCACTGTACCTGTGGACCTAGAACGCATCATGTGC
CGGCTCATCTCAGACTTCCAGCGCCACTCCATTTCCGATGACGACTCAGGCTGTGCCTCAGAGGAGTATG
CCTGGGTACCCCCTGGCCTTAAGCCGGAGCAGGTTTACCAGTTTTTCAGTTGCCTTCCAGAGGACAAGGT
CCCCTATGTCAACAGTCCTGGGGAGAAATACAGAATAAAGCAGCTACTGCACCAGCTACCCCCACACGAC
AGTGAGGCACAGTACTGCACGGCCCTGGAAGAGGAGGAGAAGAAAGAGTTGCGAGCCTTCAGCCAGCAGA
GGAAGCGGGAGAATCTGGGACGAGCCACCGTTCGAATCTTCCCAGTGACCATTACTGGGGCCATCTGCGA
GGAGTGTGGCAAGCAGATCGGCGGTGGGGACATTGCAGTCTTTGCCAGCCGCGCAGGCCTTGGTGCCTGT
TGGCACCCTCAGTGCTTTGTGTGTACCACGTGCCAGGAGCTGCTGGTTGACCTCATCTACTTCTATCATG
CTGGCAAGGTCTACTGTGGGCGCCACCATGCTGAGTGTCTGCGTCCTCGCTGCCAAGCGTGTGATGAGAT
CATCTTCTCCCCTGAATGTACGGAGGCGGAGGGGCGACACTGGCACATGGGTCACTTCTGCTGTTTCGAG
TGTGAAGCTTCGTTAGGAGGGCAGCGCTATGTCATGCGTCAGAGCCGCCCCCACTGCTGTGCCTGCTATG
AGGCCCGCCATGCGGAATACTGTGATGGCTGTGGGGAACACATCGGCCTGGACCAGGGCCAGATGGCTTA
TGAGGGCCAACACTGGCACGCTTCAGATCGCTGCTTCTGCTGTAGCCGTTGCAGTCGACCTCTTCTGGGC
CGCCCTTTCCTGCCACGCCGAGGCCTGATCTTCTGCTCACGAGCCTGCAGCCTTGGGTCTGAGACCACTG
CTCCAGGGCCCGGCCGACGCAGCTGGAGCGCCGGCACCGTCACCACACCGCTCACAACTTCTACAGCTTC
TTTCTCAGCTACGGAGGGGACATCAGAGACAGCCAGCAAAGGCACCTGTACCAAGGCAGAGCCTGCTGCA
GGCCCTGAAGAGCCCTCCCACTTTCTGAGAGGGGCCCCCCACCGCCATTCTATGCCTGAACTGGGACTCC
GCAGTGCTCCTGAGCCACCCACAGAATCCCCTGGCCATCCTGCCCCGCATCCAGATGATAATGCCTTTGG
TCGCCAAAGTACACCTCGTGTCAGCTTCCGAGACCCTCTGGTGTCTGAAGGAGGTCCACGAAGGACCCTT
AGTGCACCTCCAGCCCAGCGCCGTAGACCACGAAGTCCCCCACCCAGGACCCCCAGCTGCCACCACCACC
ACCATCATCGCCGCCGGCGCCAGCGGCACCGCCGCCGAGGGAGCCACCATCACCACCACCATCCTGGCAG
ACATGGCCACCATCGCTGTGACTTGGGATCAGGTTCAGATTCAGGATCTTGCTCCAGCTCACCCTCCAGC
CCCAGTTCAGAGTCTTCTGAGGATGATGGCTTCTTCCTGGGGGAACGTATCCCACTGCCCCCTCACCTGT
GCAGGCCCAGGACCACTCAGGACACCTCAACCGAAACCTTTAACTCCCCAGCCCAGCCACTTGTCCAGGA
GTCTCACCCCGTGATGCCTCGCCAGACCCGGGACAAGAACTGCATCGTGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR209523 protein sequence
Red=Cloning site Green=Tags(s)

MFARGSRRRRSGRAPPEAEDPARGQPCNSCREQCPGFLLHGWRKICQHCKCPREEHAVRTVPVDLERIMC
RLISDFQRHSISDDDSGCASEEYAWVPPGLKPEQVYQFFSCLPEDKVPYVNSPGEKYRIKQLLHQLPPHD
SEAQYCTALEEEEKKELRAFSQQRKRENLGRATVRIFPVTITGAICEECGKQIGGGDIAVFASRAGLGAC
WHPQCFVCTTCQELLVDLIYFYHAGKVYCGRHHAECLRPRCQACDEIIFSPECTEAEGRHWHMGHFCCFE
CEASLGGQRYVMRQSRPHCCACYEARHAEYCDGCGEHIGLDQGQMAYEGQHWHASDRCFCCSRCSRPLLG
RPFLPRRGLIFCSRACSLGSETTAPGPGRRSWSAGTVTTPLTTSTASFSATEGTSETASKGTCTKAEPAA
GPEEPSHFLRGAPHRHSMPELGLRSAPEPPTESPGHPAPHPDDNAFGRQSTPRVSFRDPLVSEGGPRRTL
SAPPAQRRRPRSPPPRTPSCHHHHHHRRRRQRHRRRGSHHHHHHPGRHGHHRCDLGSGSDSGSCSSSPSS
PSSESSEDDGFFLGERIPLPPHLCRPRTTQDTSTETFNSPAQPLVQESHPVMPRQTRDKNCIVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175097
ORF Size 1872 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 2376 bp
RefSeq ORF 1671 bp
Locus ID 54630
Cytogenetics X 3.45 cM
MW 69.7 kDa
Summary Involved in the planar cell polarity (PCP) pathway that is essential for the polarization of epithelial cells during morphogenetic processes, including gastrulation and neurulation (By similarity). PCP is maintained by two molecular modules, the global and the core modules, PRICKLE3 being part of the core module (By similarity). Distinct complexes of the core module segregate to opposite sides of the cell, where they interact with the opposite complex in the neighboring cell at or near the adherents junctions (By similarity). Involved in the organization of the basal body (By similarity). Involved in cilia growth and positioning (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175097" in other vectors (3)
SKU Description Size Price
MC218922 Prickle3 (untagged) - Mouse prickle homolog 3, (10ug) 10 ug
$570.00
MR209523L3 Lenti ORF clone of Prickle3 (Myc-DDK-tagged) - Mouse prickle homolog 3 10 ug
$882.00
MR209523L4 Lenti ORF clone of Prickle3 (mGFP-tagged) - Mouse prickle homolog 3 10 ug
$882.00

Plasmid Map for MR209523

Download sequence for

Cloning scheme for MR209523

Circular map for MR209523

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