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Dclk3 (BC056929) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209474
Dclk3 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $576.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dclk3
Synonyms BC056929; C730036H08; Dcamkl3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209474 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAAAGAGCCGCTGACATTGAAGAGTATCCAGTTGGCCATGGAGGAGCTGTATCCTAAGAACCGGG
CTCTTGCCCTGGCCCCTCACAGTAGAGTCCCCTCCCCAAGGCTGAGAAGCAGACTTCCCAGCAAGCTTCT
GAAAGGAAGTCACCGCTGTGGGGAGGCAGGAAGCTATAGCGCGGAAATGGAGAGTAAGGCAGTCTCTAGG
CATCAGGGCAAGACTTCCACAGTGCTGGCCCCAGAAGACAAGGCGAGGGCCCAGAAGTGGGTAAGAGGGA
AACAGGAGTCAGAACCTGGTGGCCCGCCTTCACCCGGGGCAGCCACTCAGGAGGAGACTCATGCAAGTGG
AGAGAAACATCTGGGGGTGGAGATCGAAAAGACCTCCGGGGAGATTGTCAGATGTGAGAAGTGTAAGAGA
GAAAGAGAGCTGCAGTTGGGCCTGCAGAGGGAGCCGTGCCCGCTGGGAACCAGTGAGCTGGACCTGGGGA
GAGCTCAGAAGAGGGATTCCGAGAAGTTGGTGAGGACCAAGAGCTGCAGGAGGCCTTCTGAGGCAAAATC
TACAGATGGAGAGGAAGGGTGGAAGGGTGACAGCCATCGGGGCAGTCCCAGGGACCCCCCTCAGGAACTG
AGGAGGCCCAACAGCAACTCAGACAAGAAAGAGATCAGAGGCTCAGAAAGTCAGGACAGTCATCCTCAGG
GGGCACCCAAGGCCCAGAAGGACCTCGTGGAAGGGCCACCAGCTGTAGAGGAGGGGCCGATAGACATGAG
GAGAGAGGACCGGCACACATGCAGGAGCAAGCATGCCGCCTGGCTCCGGAGAGAGCAGCAGGCCGAACCC
CCACAGCTCCCCAGAACCCGAGGGGAGGAGAAGCAAGCAGAGCACGAGAAGAAGCCAGGCGGCTTAGGAG
AGAGGAGGGCGCCAGAGAAGGAGTCTAAGAGGAAGCTAGAAGAGAAGAGGCCAGAACGACCCAGTGGCCG
GAAGCCGAGGCCCAAGGGCATCATCTCAGCGGATGTGGAGAAGCACTATGACATAGGTGGGGTCATTGGG
GATGGCAACTTTGCCACCGTGAAGGAATGCAGGCACCGAGAGACCAAGCAGGCTTACGCCATGAAGATGA
TTGACAAGTCCCAGCTGAAGGGTAAGGAGGACATTGTCGACAGTGAGATTTTAATCATCCAGAGTCTCTC
TCATCCCAACATTGTGAAACTGCACGAGGTCTACGAGACGGAGGCGGAGATCTACCTGATCATGGAGTAT
GTGCAGGGAGGGGACCTTTTTGATGCCATCGTTGAAAATGTGAAGTTTCCAGAGCCCGAGGCTGCAGTTA
TGATCACAGACTTGTGTAAGGCCCTCGTCCACATGCACGACAAGAATATCGTCCACCGGGACGTGAAACC
AGAAAACCTCCTGGTTCAGCGAAATGAAGACAAGTCTATCACCTTGAAGCTGGCTGATTTTGGCTTGGCC
AAATATGTGGTGAGGCCTATATTTACTGTGTGTGGGACGCCAACATATGTAGCTCCTGAAATTCTTTCTG
AGAAAGGTTACGGCCTGGAGGTGGACATGTGGGCGGCAGGTGTGATCCTATACATCCTCTTGTGTGGCTT
CCCCCCTTTCCGAAGTCCTGAGAGGGACCAAGACGAGCTCTTCAACATCATCCAAGTGGGCCAGTTTGAG
TTCCTCTCTCCTTACTGGGACAACATTTCTGATGCTGCCAAAGATCTGGTGAGAAATTTGCTGGAGGTGG
ACCCTAAGAAGCGGTACACGGCCGAACAGGTCCTACAGCATCCCTGGATTGAGATGGTTGGGCATACCAA
CACAGGGAACTCACAGAAGGAGGAGTCCCCCAACAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209474 protein sequence
Red=Cloning site Green=Tags(s)

MGKEPLTLKSIQLAMEELYPKNRALALAPHSRVPSPRLRSRLPSKLLKGSHRCGEAGSYSAEMESKAVSR
HQGKTSTVLAPEDKARAQKWVRGKQESEPGGPPSPGAATQEETHASGEKHLGVEIEKTSGEIVRCEKCKR
ERELQLGLQREPCPLGTSELDLGRAQKRDSEKLVRTKSCRRPSEAKSTDGEEGWKGDSHRGSPRDPPQEL
RRPNSNSDKKEIRGSESQDSHPQGAPKAQKDLVEGPPAVEEGPIDMRREDRHTCRSKHAAWLRREQQAEP
PQLPRTRGEEKQAEHEKKPGGLGERRAPEKESKRKLEEKRPERPSGRKPRPKGIISADVEKHYDIGGVIG
DGNFATVKECRHRETKQAYAMKMIDKSQLKGKEDIVDSEILIIQSLSHPNIVKLHEVYETEAEIYLIMEY
VQGGDLFDAIVENVKFPEPEAAVMITDLCKALVHMHDKNIVHRDVKPENLLVQRNEDKSITLKLADFGLA
KYVVRPIFTVCGTPTYVAPEILSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQVGQFE
FLSPYWDNISDAAKDLVRNLLEVDPKKRYTAEQVLQHPWIEMVGHTNTGNSQKEESPNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC056929
ORF Size 1857 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC056929, AAH56929
RefSeq Size 2692 bp
RefSeq ORF 1859 bp
Locus ID 245038
Cytogenetics 9 F3
MW 69.6 kDa
Summary This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. provided by RefSeq, Sep 2010
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"BC056929" in other vectors (4)
SKU Description Size Price
MC218040 Dclk3 (untagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292), (10ug) 10 ug
$635.00
MG209474 Dclk3 (tGFP-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292) 10 ug
$776.00
MR209474L3 Lenti ORF clone of Dclk3 (Myc-DDK-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292) 10 ug
$876.00
MR209474L4 Lenti ORF clone of Dclk3 (mGFP-tagged) - Mouse doublecortin-like kinase 3 (cDNA clone MGC:66532 IMAGE:6816292) 10 ug
$876.00

Plasmid Map for MR209474

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209474
Circular map for MR209474

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