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Rbm14 (BC010294) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209471
Rbm14 (Myc-DDK-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $575.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rbm14
Synonyms p16K, Sytip, p16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATTTTTGTGGGCAATGTCGATGGGGCGGATACGACACCGGAGGAGTTGGCAGCTCTCTTCGCGC
CCTATGGCACGGTCATGAGCTGCGCCGTCATGAAACAGTTTGCCTTCGTGCACATGCGCGAGAACGCTGG
CGCGGTGCGCGCCATCGAGGCCCTGCATGGCCACGAGCTGCGTCCAGGTCGCGCGCTCGTGGTGGAGATG
TCGCGCCCGAGGCCCCTGAACACTTGGAAGATTTTCGTGGGCAATGTATCGGCTGCATGTACAAGTCAGG
AATTGCGCAGCCTCTTCGAGCGCCGTGGACGCGTCATCGAGTGTGACGTGGTAAAAGACTACGCGTTTGT
TCACATGGAGAAGGAAGCAGATGCCAAAGCCGCCATCGCGCAGCTCAACGGCAAAGAAGTGAAGGGCAAG
CGCATCAACGTGGAACTCTCAACCAAGGGGCAGAAGAAGGGGCCTGCCCTGGCTATCCAGTCTGGGGACA
AGACCAAGAAACCAGGGGCTGGGGACACAGCATTCCCTGGAACTGGTGGCTTCTCTGCCACCTTCGACTA
CCAGCAGGCTTTTGGCAACAGCACTGGTGGCTTTGATGGGCAAGCCCGTCAGCCCACACCACCATTCTTT
GGTCGCGACCGCAGCCCCCTGCGCCGTTCACCTCCTCGAGCCTCTTATGTGGCGCCTCTGACGGCCCAGC
CAGCCACCTACCGGGCCCAACCCTCAGTGTCTCTGGGAGCTGCATATAGGGCCCAGCCTTCCGCCTCTTT
GGGTGTCGGCTATCGGACTCAGCCCATGGCAGCTCAGGCAGCTTCTTACCGTGCTCAGCCCTCTGTCTCC
CTTGGGGCCCCATACAGGGGTCAGCTGGCTAGCCCTAGTTCCCAGTCTGCTGCAGCTTCCTCGCTTGGCC
CATATGGTGGAGTCCAGCCCTCAGCCTCAGCCCTCTCCACTTATGGGGGTCAGGCAGCTGCAGCTTCTTC
GCTTAATTCCTATGGGGCTCAGGGCTCCTCCCTTGCTTCCTATGGTAACCAGCCATCCTCTTATGGCGCC
CAGGCTGCCTCTTCGTATGGGGTTCGTGCGGCTGCTTCTTCCTATAACACACAAGGAGCAGCTTCTTCCC
TAGGTTCCTATGGGGCTCAAGCAGCCTCCTATGGTGCCCAGTCTGCAGCCTCCTCACTAGCTTATGGAGC
CCAGGCAGCGTCTTACAGTGCCCAGCCCTCAGCCTCTTACAGTGCCCAGTCTGCCCCATATGCTGCACAA
CAGGCTGCTTCTTATTCTTCCCAGCCTGCTGCTTATGTGGCACAACCAGCCACAGCTGCTGCCTATGCTA
GCCAGCCAGCTGCCTATGCTGCACAAGCCACTACCCCAATGGCTGGTTCCTATGGGGCTCAACCAGTTGT
TCAGACCCAGCTGAATAGCTATGGGGCTCAAGCATCTATTGGCCTGTCAGGCTCATATGGAGCTCAGTCT
GCTGCTGCGGCCACTGGCTCCTATGGGGCGGCAGCTGCTTACGGGGCTCAGCCTTCTGCCACCCTGGCAG
CTCCTTACCGCACTCAGTCATCAGCCTCATTGGCTGCTTCCTATGCTGCCCAGCAGCATCCCCAGGCTGC
TGCCTCCTACCGTGGCCAGCCGGGCAGTGCCTACGATGGGACAGGCCAGCCATCAGCAGCCTACCTGTCT
ATGTCCCAGGGGGCCGTTGCCAACGCCAACAGCACCCCGCCGCCCTATGAGCGTACCCGCCTCTCCCCAC
CCCGGGCCAGCTACGACGATCCCTACAAAAAGGCTGTCGCCATGTCAAAAAGGTACTGTATGCCCCCCCG
CCTCAGCCCCCAGCTGGGGCTTAGGGCAAGGGGC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209471 protein sequence
Red=Cloning site Green=Tags(s)

MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGAVRAIEALHGHELRPGRALVVEM
SRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGK
RINVELSTKGQKKGPALAIQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFF
GRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMAAQAASYRAQPSVS
LGAPYRGQLASPSSQSAAASSLGPYGGVQPSASALSTYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGA
QAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYSAQPSASYSAQSAPYAAQ
QAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASIGLSGSYGAQS
AAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGSAYDGTGQPSAAYLS
MSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYCMPPRLSPQLGLRARG

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN BC010294
ORF Size 1854 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC010294, AAH10294
RefSeq Size 3545 bp
RefSeq ORF 1856 bp
Locus ID 56275
Cytogenetics 19 A
MW 63.2 kDa
Summary May function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1 (By similarity). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity (PubMed:25385835). Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.UniProtKB/Swiss-Prot Function
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"BC010294" in other vectors (4)
SKU Description Size Price
MC200669 Rbm14 (untagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900), (10ug) 10 ug
$576.00
MG209471 Rbm14 (tGFP-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900) 10 ug
$775.00
MR209471L3 Lenti ORF clone of Rbm14 (Myc-DDK-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900) 10 ug
$875.00
MR209471L4 Lenti ORF clone of Rbm14 (mGFP-tagged) - Mouse RNA binding motif protein 14 (cDNA clone MGC:11556 IMAGE:3153900) 10 ug
$875.00

Plasmid Map for MR209471

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209471
Circular map for MR209471

file-alt Citations

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