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Mtmr6 (NM_144843) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209453
Mtmr6 (Myc-DDK-tagged) - Mouse myotubularin related protein 6 (Mtmr6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $575.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mtmr6
Synonyms 4022440C11Rik; AI428804; AU041072
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209453 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCACATCCGGACAACCAAGGTTGAACAAGTAAAGTTACTTGACCGGTTTAGTACCAACAACAAGT
CACTGACAGGAACACTGTATCTTACGGCCACACACCTATTATTTATTGACGCTCAGCAAAAAGAAACCTG
GATACTACACCACCATATTGCCTCTGTGGAGAAACTTGCTCTGACCACTTCTGGATGCCCACTTGTGATT
CAGTGCAAGAACTTCAGGATTGTGCATTTCATTGTTCCCAGGGAAAGAGATTGCCACGATATTTATAACT
CTCTCCTGCAGCTATCAAAACAAGCAAAATATGAAGATCTCTATGCCTTCTCTTATAATCCCAAGCAAAA
TGATACTGAACGACGGAATGGTTGGCAGCTTATTGACCTTGCTGCGGAGTATGAGAGGATGGGAGTGCCC
AATGCAAACTGGCAACTGTCGGATGCCAACCGAGAGTACAAGGTCTGTGAGACTTATCCCAGAGAGCTGT
ATGTTCCTCGGACAGCTAGCCGGCCGGTGATCGTGGGCAGTTCCAACTTCCGGAGCAAGGGCAGACTTCC
GGTCCTATCCTACTGCCGGCAGGGCACTGAGGCTGCCATTTGTCGATGTAGTCAGCCATTGTCAGGCTTC
AGTGCCAGGTGCCTAGAGGATGAGCATTTGCTTCAAGCCATTAGTAAAGCCAACCCAGGAAACCGCTACA
TGTATGTTGTGGATACCAGGCCCAAGCTCAATGCAATAGCTAACAGAGCAGCTGGAAAAGGTTATGAAAA
TGAAGACAACTATTCTAATATTAGATTTCAGTTCGTTGGAATAGAAAATATTCATGTCATGAGATCCAGC
CTTCAGAAATTACTGGAAGTGAATGGTAGCAAGGGGCTTTCTGTCAATGATTTCTACTCTGGTTTGGAAA
GTTCAGGATGGCTTCGCCATATCAAGGCTGTTTTGGATGCTGCAATCTTCTTAGCCAAAGCAATAGTGGT
TGAAAATGCAAGTGTGTTGGTCCACTGTTCCGATGGGTGGGACAGGACCTCCCAAGTGTGCTCCCTCGGC
TCACTGTTGTTGGATTCCTACTACAGGACAATGAAAGGATTCATGGTTTTAATAGAAAAAGATTGGATAT
CATTTGGGCATAAATTTTCAGAGAGGTGTGGCCATTTGGATGGAGACCCGAGGGAGGTCTCACCAGTGTT
CACTCAGTTCTTGGAGTGTGTCTGGCACCTGACTCAGCAGTTCCCACAAGCCTTCGAGTTCAACGAAGCG
TTTCTTCTTCAGATCCATGAGCACATTCATTCATGCCAATTTGGGAACTTCCTTGGCAACTGTCAGAAGG
AGAGAGAGGAACTCAGGTTAAAGGAGAAAACTTACTCCCTGTGGCCTTTTCTTTTGGATGACAAAAAGAA
GTACTTAAATCCTCTCTACAGTTCCAAATCTCAGAGACTGACAGTCTTGGAGCCAAATACAGCCTCTTTC
AATTTTAAGTTTTGGAGAAACATGTACCACCAGTTTGATCGGACGCTCCATCCTCGGCAGTCTGTGCTGA
GCATAATTATGAATATGAATGAGCAAAGCAAGCAGTTAGAGGAAGACATTAAAGACCTCGAGGCTAAAAT
TAAGCAATGCAAAAATGGCATCCTAACGAAGGAGTTGCTGCATGCTGTCCACCCTGAATCACCTGCCCTG
AAAACCTCGCTGTGTCTCAAAGAGCCGAGTCTGCTTCCCGTGAAGGACACACTCCGAGCTATAGAGGGCA
GCAGCCCAGCAGATAATCGCTACTGTGACTATGCCGAGGAATTTTCTAAGTCGGAGCCTACCGTGGTCAG
CTTGGAGTACGGGGTGGCCAGAATGACTTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209453 protein sequence
Red=Cloning site Green=Tags(s)

MEHIRTTKVEQVKLLDRFSTNNKSLTGTLYLTATHLLFIDAQQKETWILHHHIASVEKLALTTSGCPLVI
QCKNFRIVHFIVPRERDCHDIYNSLLQLSKQAKYEDLYAFSYNPKQNDTERRNGWQLIDLAAEYERMGVP
NANWQLSDANREYKVCETYPRELYVPRTASRPVIVGSSNFRSKGRLPVLSYCRQGTEAAICRCSQPLSGF
SARCLEDEHLLQAISKANPGNRYMYVVDTRPKLNAIANRAAGKGYENEDNYSNIRFQFVGIENIHVMRSS
LQKLLEVNGSKGLSVNDFYSGLESSGWLRHIKAVLDAAIFLAKAIVVENASVLVHCSDGWDRTSQVCSLG
SLLLDSYYRTMKGFMVLIEKDWISFGHKFSERCGHLDGDPREVSPVFTQFLECVWHLTQQFPQAFEFNEA
FLLQIHEHIHSCQFGNFLGNCQKEREELRLKEKTYSLWPFLLDDKKKYLNPLYSSKSQRLTVLEPNTASF
NFKFWRNMYHQFDRTLHPRQSVLSIIMNMNEQSKQLEEDIKDLEAKIKQCKNGILTKELLHAVHPESPAL
KTSLCLKEPSLLPVKDTLRAIEGSSPADNRYCDYAEEFSKSEPTVVSLEYGVARMTC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144843
ORF Size 1851 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144843.4
RefSeq Size 3845 bp
RefSeq ORF 1854 bp
Locus ID 219135
UniProt ID Q8VE11
Cytogenetics 14 D1
MW 70.9 kDa
Summary Phosphatase that acts on lipids with a phosphoinositol headgroup. Dephosphorylates phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,5-bisphosphate. Binds with high affinity to phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) but also to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), and phosphatidylinositol 5-phosphate (PtdIns(5)P), phosphatidic acid and phosphatidylserine (By similarity). Negatively regulates ER-Golgi protein transport (By similarity). Probably in association with MTMR9, plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3-phosphate in membrane ruffles (PubMed:24591580). Acts as a negative regulator of KCNN4/KCa3.1 channel activity in CD4(+) T-cells possibly by decreasing intracellular levels of phosphatidylinositol 3-phosphate. Negatively regulates proliferation of reactivated CD4(+) T-cells. In complex with MTMR9, negatively regulates DNA damage-induced apoptosis. The formation of the MTMR6-MTMR9 complex stabilizes both MTMR6 and MTMR9 protein levels (By similarity).UniProtKB/Swiss-Prot Function
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"NM_144843" in other vectors (4)
SKU Description Size Price
MC201222 Mtmr6 (untagged) - Mouse myotubularin related protein 6 (Mtmr6), (10ug) 10 ug
$575.00
MG209453 Mtmr6 (tGFP-tagged) - Mouse myotubularin related protein 6 (Mtmr6) 10 ug
$775.00
MR209453L3 Lenti ORF clone of Mtmr6 (Myc-DDK-tagged) - Mouse myotubularin related protein 6 (Mtmr6) 10 ug
$875.00
MR209453L4 Lenti ORF clone of Mtmr6 (mGFP-tagged) - Mouse myotubularin related protein 6 (Mtmr6) 10 ug
$875.00

Plasmid Map for MR209453

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR209453
Circular map for MR209453

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