Vps33b (NM_178070) Mouse Tagged ORF Clone
$289.00
$575.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Vps33b |
| Synonyms | MGC36556 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_178070 |
| ORF Size | 1851 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_178070.1, NM_178070.2, NM_178070.3, NM_178070.4, NP_835171.1, NP_835171.2 |
| RefSeq Size | 2595 bp |
| RefSeq ORF | 1854 bp |
| Locus ID | 233405 |
| UniProt ID | P59016 |
| Cytogenetics | 7 D2 |
| MW | 70.5 kDa |
| Summary | May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating in part VIPAS39 (By similarity). In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependentrecycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (PubMed:25947942).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_178070" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC219766 | Vps33b (untagged) - Mouse vacuolar protein sorting 33B (yeast) (Vps33b), (10ug) | 10 ug |
$633.00
|
|
| MG209450 | Vps33b (tGFP-tagged) - Mouse vacuolar protein sorting 33B (yeast) (Vps33b) | 10 ug |
$775.00
|
|
| MR209450L3 | Lenti ORF clone of Vps33b (Myc-DDK-tagged) - Mouse vacuolar protein sorting 33B (yeast) (Vps33b) | 10 ug |
$875.00
|
|
| MR209450L4 | Lenti ORF clone of Vps33b (mGFP-tagged) - Mouse vacuolar protein sorting 33B (yeast) (Vps33b) | 10 ug |
$875.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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