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Pomt2 (BC052045) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209338
Pomt2 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $561.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pomt2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209338 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCGGCCATAGGCGGTGGCCTAGCGGGCTCCGAGCTGCGTCCCCGTAGGGGCCGCTGTGTGCCGC
AGGCTGCCAGAGCCGTGAGCCGAGACGTGGTCCCCCAGGCTGCAGCACGGAAACTTAAACGGCCTGCTTG
GAGCTCGCGGCGCTTCCAGGCGGCAGGGTGGTGGGCCACGTTGGCTGTGGTGACGCTGCTGTCCTTTGCC
ACCCGCTTTCACCGGCTGGACCAGCCTGCTCACATCTGTTGGGATGAGACTCACTTTGGAAAAATGGGAA
GTTACTATATTAACCGCACCTTTTTCTTTGATGTGCATCCACCACTGGGAAAGATGCTGATCGGTCTTGC
TGGCTACCTGAGCGGGTATGATGGTACCTTTCTGTTCCAGAAGCCTGGGGACAGATACGAGCACCACAGC
TACATGGGAATGAGAGGATTCTGTGCTTTCCTGGGTTCCTGGCTGATCCCCTTTGCCTACCTCACCGTAC
TGGATCTGTCCAAGTCCTTCCCAGCAGCACTGCTCACCGCTGCCCTTCTCACCTTTGACACAGGATGCCT
CACTCTATCCCAGTACATCCTCCTTGACCCCATCCTGATGTTCTTCATCATGGCGGCCATGCTGAGCATG
GTCAAGTACAACTCCTGTGCTAACAGGCCTTTCTCTGCCCCCTGGTGGTTCTGGCTCAGCCTGACTGGCA
TTAGTCTCGCTGGTGCTTTAGGGGTCAAATTTGTTGGCCTCTTTATCATCGTGCAAGTGGGGTTGAACAC
TATTTCAGACCTCTGGCATCTGTTTGGAGACCTCAGCCTTTCACTGGTGACTGTGGGGAAGCACCTAACT
GCTCGCATCCTGTGCCTCATAGTGCTGCCTTTGGTGCTCTATGTGACCATTTTTGCTGTTCATGTCATGG
TGCTGAATAAAAGTGGTCCTGGTGATGGCTTCTTCAGTTCTGCCTTTCAAGCCCGGCTTTCAGGGAACAG
CCTTCACAATGCTTCTATCCCTGAACACCTGGCGTATGGCTCTGTAATCACTGTGAAGAATCTCCGGATG
GCCATTGGCTACCTGCACTCTCATAGGCACCTCTACCCTGAGGGCATTGGTGCTCGCCAGCAGCAGGTTA
CCACCTATTTGCATAAGGACTACAACAACTTGTGGATTATCAAGAAGTATAATGCCAACACAGATCCCCT
AGACCCTTCATTCCCAGTGGAGTTTGTCAGACATGGAGACATCATACGACTAGAACACAAAGAAACTACT
CGGAACCTGCACAGTCACTATCATGAGGCTCCCCTGACCCGGAAGCACTATCAGGTCACTGGCTATGGCA
TAAATGGGACAGGGGACTCAAATGACTTCTGGCGGATTGAAGTTGTAAATAGAAAATTTGGGAACCGGAT
CAAGGTACTGAGAAGTCGAATTCGCCTCATCCATTTGGTCACAGGTTGTGTCCTGGGATCGTCAGGAAAG
ATTCTGCCTAAGTGGGGCTGGGAGCAGTTGGAAGTTACCTGTACTCCGTACCTAAAAGAAACCACTAACT
CCATTTGGAACATAGAAGAGCATATCAATCCCAAATTGCCAAACATCAGCCTGGATGTGCTGCAGCCCAG
TTTTCCTGAGATCTTGCTGGAGTCTCACATGGTCATGATCCGGGGAAATAATGGCCTCAAACCCAAGGAC
AATGAGTTCACATCCAAGCCCTGGCACTGGCCTATCAATTATCAGGGCCTGCGCTTCTCAGGGGCCAATG
ACACGGACTTCCGAGTCTATCTGCTCGGCAACCCTGTGAGTATCCCAGCTCTGAGTGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209338 protein sequence
Red=Cloning site Green=Tags(s)

MPPAIGGGLAGSELRPRRGRCVPQAARAVSRDVVPQAAARKLKRPAWSSRRFQAAGWWATLAVVTLLSFA
TRFHRLDQPAHICWDETHFGKMGSYYINRTFFFDVHPPLGKMLIGLAGYLSGYDGTFLFQKPGDRYEHHS
YMGMRGFCAFLGSWLIPFAYLTVLDLSKSFPAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSM
VKYNSCANRPFSAPWWFWLSLTGISLAGALGVKFVGLFIIVQVGLNTISDLWHLFGDLSLSLVTVGKHLT
ARILCLIVLPLVLYVTIFAVHVMVLNKSGPGDGFFSSAFQARLSGNSLHNASIPEHLAYGSVITVKNLRM
AIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKYNANTDPLDPSFPVEFVRHGDIIRLEHKETT
RNLHSHYHEAPLTRKHYQVTGYGINGTGDSNDFWRIEVVNRKFGNRIKVLRSRIRLIHLVTGCVLGSSGK
ILPKWGWEQLEVTCTPYLKETTNSIWNIEEHINPKLPNISLDVLQPSFPEILLESHMVMIRGNNGLKPKD
NEFTSKPWHWPINYQGLRFSGANDTDFRVYLLGNPVSIPALSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC052045
ORF Size 1809 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052045, AAH52045
RefSeq Size 2800 bp
RefSeq ORF 1811 bp
Locus ID 217734
Cytogenetics 12 D2
MW 67.9 kDa
Summary This gene encodes an integral membrane protein that belongs to the dolichyl-phosphate-mannose-protein mannosyltransferase family. The encoded enzyme is found in the membrane of the endoplasmic reticulum. This protein is a component of the protein O-mannosyltransferase enzyme complex which is involved in modification of the protein alpha-dystroglycan. Mutations in the human gene are a cause of different forms of muscular dystrophy-dystroglycanopathy (MDDG), type A2 (also known as Walker-Warburg syndrome), type B2 and type C2 (also known as limb-girdle muscular dystrophy). provided by RefSeq, Sep 2015
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"BC052045" in other vectors (4)
SKU Description Size Price
MC217986 Pomt2 (untagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328), (10ug) 10 ug
$618.00
MG209338 Pomt2 (tGFP-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328) 10 ug
$761.00
MR209338L3 Lenti ORF clone of Pomt2 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328) 10 ug
$861.00
MR209338L4 Lenti ORF clone of Pomt2 (mGFP-tagged) - Mouse protein-O-mannosyltransferase 2 (cDNA clone MGC:62419 IMAGE:6402328) 10 ug
$861.00

Plasmid Map for MR209338

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209338
Circular map for MR209338

file-alt Citations

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