Nr4a1 (NM_010444) Mouse Tagged ORF Clone

SKU
MR209316
Nr4a1 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$289.00 MSRP $560.00 MSRP $560.00
In Stock*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nr4a1
Synonyms Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; nur77; TIS1; TR3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_010444
ORF Size 1806 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010444.2
RefSeq Size 2534 bp
RefSeq ORF 1806 bp
Locus ID 15370
UniProt ID P12813
Cytogenetics 15 F1
MW 64.7 kDa
Summary Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity). Plays a role in the vascular response to injury (PubMed:22427340).[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
MC200008 Nr4a1 (untagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1), (10ug) 10 ug
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MG209316 Nr4a1 (tGFP-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1) 10 ug
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MR209316L1 Lenti ORF clone of Nr4a1 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1) 10 ug
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MR209316L2 Lenti ORF clone of Nr4a1 (mGFP-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1) 10 ug
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MR209316L3 Lenti ORF clone of Nr4a1 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1) 10 ug
$860.00
MR209316L4 Lenti ORF clone of Nr4a1 (mGFP-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1) 10 ug
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Citations

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