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Zfp143 (BC037658) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR209289
Zfp143 (Myc-DDK-tagged) - Mouse zinc finger protein 143 (cDNA clone MGC:46861 IMAGE:5356405)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $557.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp143
Synonyms AA959806; AU015869; D7Ertd805e; KRAB14; pHZ-1; SBF; Staf; Zfp79; Zfp80-rs1; Znf143
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR209289 representing BC037658
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGAATTTCCTGGAGGAGGAATGGAGGCTCAACATGTTACCCTGTGCCTGACGGAGGCAGTAACCG
TGGCAGATGGTGACAATTTAGAAAATATGGAAGGTGTGAGTTTACAAGCTGTGACACTCGCAGACGGCTC
TACTGCTTACATACAGCATAATTCTAAAGACGGGAGACTCATTGATGGCCAGGTCATTCAGCTGGAAGAT
GGTTCTGCTGCCTATGTTCAGCATGTCCCCATACCTAAAAGTAACAGTTATGACCAGAGCTCACTTCAGG
CCGTTCAGTTAGAAGATGGGACAACAGCTTATATCCACCATGCAGTGCAAGTCCCACAGTCTGATACCAT
CTTGGCAATTCAGGCTGATGGGACAGTGGCAGGGCTGCACACTGGGGATGCCACAATTGACCCAGATACC
ATTAGTGCTTTGGAGCAGTACGCAGCAAAGGTATCCATTGATGGCAGTGACGGTGTAACAAGTACAGGAA
TGATTGGAGAGAATGAGCAAGAGAAAAAAATGCAGATTGTCTTACAAGGCCATGCTACTCGGGTAACTCC
TAAATCTCAGCAGAGTGGAGAAAAGGCATTTCGGTGCAAATACGACGGCTGTGGGAAACTCTATACAACA
GCTCATCACCTTAAGGTCCATGAGCGCTCACACACAGGAGACCGGCCTTACCAGTGTGAGCATTCAGGCT
GTGGGAAAGCATTTGCAACAGGTTATGGATTAAAAAGTCATTTCAGAACTCATACTGGAGAAAAGCCATA
TCGGTGTTCAGAAGATAATTGTACAAAGTCTTTCAAAACTTCAGGAGATCTACAGAAGCACATCAGAACC
CATACAGGAGAAAGGCCCTTTAAGTGTCCTATTGAAGGCTGTGGTCGGTCCTTTACCACATCAAATATCA
GAAAAGTGCACATTAGGACACACACAGGAGAAAGACCTTATTACTGCACAGAACCTGGATGTGGGAGGGC
ATTTGCCAGCGCAACTAATTATAAAAACCATGTGAGGATACACACAGGAGAAAAGCCATATGTCTGTACA
GTTCCTGGGTGTGACAAACGGTTTACAGAATATTCCAGTTTGTATAAACACCATGTCGTTCACACTCACT
CTAAACCATACAACTGTAACCACTGTGGGAAGACGTACAAACAGATTTCCACACTGGCCATGCACAAACG
GACAGCCCACAACGACACAGAGCCCATTGAGGAGGAGCAGGAAGCCTTCTTCGAGCCTCCCCCAGGTCAA
GGTGATGATGTTCTTAAAGGGTCCCAGATCACATATGTTACAGGTGTAGATGGAGAGGACATTGTATCTA
CACAAGTAGCCACAGTAACCCAGTCTGGGCTGAGTCAACAAGTCACACTCATATCACAGGATGGGACTCA
ACATGTCAACATATCTCAAGCTGACATGCAGGCCATTGGCAACACCATCACCATGGTAACACAGGATGGC
ACACCCATCACGGTCCCCACTCACGATGCAGTCATCTCCTCAGCAGGAACACATTCTGTTGCTATGGTTA
CCGCGGAGGGCACAGAAGGACAGCAGGTCGCAATTGTAGCTCAAGATCTGGCAGCGTTCCATACAGCATC
TTCAGAGATGGGACACCAACAACATAGCCATCACCTAGTAACTACAGAAACCAGACCTCTGACCTTAGTG
GCAACATCCAACGGCACCCAGATCGCAGTTCAGCTTGGAGAGCAGCCGTCTCTGGAAGAAGCTATCAGAA
TAGCATCGAGGATCCAACAAGGGGAGACGCCAGGGTTGGATGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR209289 representing BC037658
Red=Cloning site Green=Tags(s)

MTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGSTAYIQHNSKDGRLIDGQVIQLED
GSAAYVQHVPIPKSNSYDQSSLQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDT
ISALEQYAAKVSIDGSDGVTSTGMIGENEQEKKMQIVLQGHATRVTPKSQQSGEKAFRCKYDGCGKLYTT
AHHLKVHERSHTGDRPYQCEHSGCGKAFATGYGLKSHFRTHTGEKPYRCSEDNCTKSFKTSGDLQKHIRT
HTGERPFKCPIEGCGRSFTTSNIRKVHIRTHTGERPYYCTEPGCGRAFASATNYKNHVRIHTGEKPYVCT
VPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLAMHKRTAHNDTEPIEEEQEAFFEPPPGQ
GDDVLKGSQITYVTGVDGEDIVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDG
TPITVPTHDAVISSAGTHSVAMVTAEGTEGQQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLV
ATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC037658
ORF Size 1794 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC037658.1
RefSeq Size 2980 bp
RefSeq ORF 1796 bp
Locus ID 20841
Cytogenetics 7 57.7 cM
MW 109.2 kDa
Summary Transcriptional activator. Activates the gene for selenocysteine tRNA (tRNAsec). Binds to the SPH motif of small nuclear RNA (snRNA) gene promoters. Participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8 (By similarity).UniProtKB/Swiss-Prot Function
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"BC037658" in other vectors (4)
SKU Description Size Price
MC218247 Zfp143 (untagged) - Mouse zinc finger protein 143 (cDNA clone MGC:46861 IMAGE:5356405), (10ug) 10 ug
$613.00
MG209289 Zfp143 (tGFP-tagged) - Mouse zinc finger protein 143 (cDNA clone MGC:46861 IMAGE:5356405) 10 ug
$757.00
MR209289L3 Lenti ORF clone of Zfp143 (Myc-DDK-tagged) - Mouse zinc finger protein 143 (cDNA clone MGC:46861 IMAGE:5356405) 10 ug
$857.00
MR209289L4 Lenti ORF clone of Zfp143 (mGFP-tagged) - Mouse zinc finger protein 143 (cDNA clone MGC:46861 IMAGE:5356405) 10 ug
$857.00

Plasmid Map for MR209289

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR209289
Circular map for MR209289

file-alt Citations

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